A5075576544- Muscle metabolism and nutrition
- Sports Performance and Training
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Sports injuries and prevention
- Cardiovascular and exercise physiology
- Adipose Tissue and Metabolism
- Genetics and Physical Performance
- Nutrition and Health in Aging
- Chromosomal and Genetic Variations
- Diet and metabolism studies
- Body Composition Measurement Techniques
- CRISPR and Genetic Engineering
- Sirtuins and Resveratrol in Medicine
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Vitamin D Research Studies
- Vitamin C and Antioxidants Research
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Inflammasome and immune disorders
- Tryptophan and brain disorders
- Thermoregulation and physiological responses
- Muscle activation and electromyography studies
Colorado State University
2023-2025
Auburn University
2016-2024
Applied Sciences Laboratory (United States)
2018-2020
We sought to determine the effects of L-leucine (LEU) or different protein supplements standardized LEU (~3.0 g/serving) on changes in body composition, strength, and histological attributes skeletal muscle adipose tissue. Seventy-five untrained, college-aged males (mean ± standard error mean (SE); age = 21 1 years, mass 79.2 0.3 kg) were randomly assigned an isocaloric, lipid-, organoleptically-matched maltodextrin placebo (PLA, n 15), (n 14), whey concentrate (WPC, 17), hydrolysate (WPH,...
We sought to identify biomarkers which delineated individual hypertrophic responses resistance training. Untrained, college-aged males engaged in full-body training (3 d/wk) for 12 weeks. Body composition via dual x-ray absorptiometry (DXA), vastus lateralis (VL) thickness ultrasound, blood, VL muscle biopsies, and three-repetition maximum (3-RM) squat strength were obtained prior (PRE) following (POST) weeks of K-means cluster analysis based on changes identified LOW [n = 17; change...
Cellular adaptations that occur during skeletal muscle hypertrophy in response to high-volume resistance training are not well-characterized. Therefore, we sought explore how actin, myosin, sarcoplasmic protein, mitochondrial, and glycogen concentrations were altered individuals exhibited mean fiber cross-sectional area (fCSA) following 6 weeks of training. Thirty previously resistance-trained, college-aged males (mean ± standard deviation: 21±2 years, 5±3 years) had vastus lateralis (VL)...
We sought to determine the skeletal muscle genome-wide DNA methylation and mRNA responses one bout of lower load (LL) versus higher (HL) resistance exercise. Trained college-aged males (
Abstract Aging is the primary risk factor for most neurodegenerative diseases, including Alzheimer's disease. Major hallmarks of brain aging include neuroinflammation/immune activation and reduced neuronal health/function. These processes contribute to cognitive dysfunction (a key disease), but their upstream causes are incompletely understood. Age‐related increases in transposable element (TE) transcripts might function with aging, as reverse transcriptase inhibitor 3TC reduces inflammation...
Abstract Neuroinflammation contributes to impaired cognitive function in brain aging and neurodegenerative disorders like Alzheimer’s disease, which is characterized by the aggregation of pathological tau. One major driver both age- tau-associated neuroinflammation NF-κB NLRP3 signaling axis. However, current treatments targeting or may have adverse/systemic effects, most not been clinically translatable. In this study, we tested efficacy a novel, nucleic acid therapeutic (Nanoligomer)...
We examined if resistance training affected muscle NAD+ and NADH concentrations as well nicotinamide phosphoribosyltransferase (NAMPT) protein levels sirtuin (SIRT) activity markers in middle-aged, untrained (MA) individuals. MA participants (59±4 years old; n=16) completed 10 weeks of full-body (2 d/wk). Body composition, knee extensor strength, vastus lateralis biopsies were obtained prior to (Pre) 72 hours following the last bout (Post). Data from trained college-aged men (22±3 old, age:...
Limited evidence exists regarding differentially expressed biomarkers between previously-trained low versus high hypertrophic responders in response to resistance training. Herein, 30 college-aged males (training age 5 ± 3 years; mean SD) partook 6 weeks of high-volume Body composition, right leg vastus lateralis (VL) biopsies, and blood were obtained prior training (PRE) at the 3-week (W3) 6-week time points (W6). The 10 lowest (LOW) highest (HIGH) clustered based upon a composite...
We investigated the acute and chronic effects of resistance training (RT) on skeletal muscle markers mitochondrial content remodeling in older, untrained adults. Sixteen participants (n = 6 males, n 10 females; age 59 ± 4 years) completed weeks full-body RT (2 day/week). Muscle biopsies from vastus lateralis were obtained prior to (Pre), 24 hr following first session (Acute), 72 last (Chronic). Protein levels electron transport chain complexes I–V (+39 +180%, p ≤ .020) fusion Mfn1 (+90%,...
Resistance training (RT) dynamically alters the skeletal muscle nuclear DNA methylome. However, no study has examined if RT affects mitochondrial (mtDNA) Herein, ten older, Caucasian untrained males (65 ± 7 y.o.) performed six weeks of full-body (twice weekly). Body composition and knee extensor torque were assessed prior to 72 h following last session. Vastus lateralis (VL) biopsies also obtained. VL was subjected reduced representation bisulfite sequencing providing excellent coverage...
What is the central question of this study? Do changes in myofibre cross-sectional area, pennation angle and fascicle length predict vastus lateralis whole-muscle area following resistance training? main finding its importance? Changes mean a period training did not collectively area. Despite limited sample size study, these data reiterate that it remains difficult to generalize morphological adaptations predominantly drive tissue-level muscle hypertrophy.Myofibre hypertrophy during (RT)...
Abstract We investigated the effects of performing a period resistance training (RT) on performance and molecular adaptations to subsequent endurance (ET). Twenty‐five young adults were divided into an RT+ET group ( n = 13), which underwent 7 weeks RT followed by ET, ET‐only 12), performed ET. Body composition, muscle biopsies collected before (T1, baseline for RT+ET), ET (T2, after ET) (T3). Immunohistochemistry was determine fibre cross‐sectional area (fCSA), myonuclear content, domain...
We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers mitochondrial physiology in high versus low anabolic responders.Untrained college-aged males were classified as responders the top 25th percentile (high-response cluster (HI); n = 13, dual x-ray absorptiometry total body mass change (Δ) +3.1 ± 0.3 kg, Δ vastus lateralis (VL) thickness +0.59 0.05 cm, fiber cross sectional area +1,426...
We examined hypertrophic outcomes of weekly graded whey protein dosing (GWP) vs. (WP) or maltodextrin (MALTO) dosed once daily during 6 weeks high-volume resistance training (RT). College-aged resistance-trained males (training age = 5 ± 3 years; mean SD) performed RT wherein frequency was d/week and each session involved 2 upper- lower-body exercises (10 repetitions/set). Volume increased from 10 sets/exercise (week 1) to 32 6), which is the highest volume investigated in this timeframe....
ABSTRACT Neuroinflammation contributes to impaired cognitive function in brain aging and neurodegenerative disorders like Alzheimer’s disease, which is characterized by the aggregation of pathological tau. One major driver both age- tau-associated neuroinflammation NF-κB NLRP3 signaling axis. However, current treatments targeting or may have adverse/systemic effects, most not been clinically translatable. In this study, we tested efficacy a novel, nucleic acid therapeutic (Nanoligomer)...
Resistance training increases muscle fiber hypertrophy, but the morphological adaptations that occur within fibers remain largely unresolved. Fifteen males with minimal experience (24±4years, 23.9±3.1kg/m 2 body mass index) performed 10weeks of conventional, full-body resistance (2× weekly). Body composition, radiological density vastus lateralis using peripheral quantitative computed tomography (pQCT), and biopsies were obtained 1week prior to 72h following last bout. Quantification...
This is the first study to comprehensively examine how different midthigh muscle imaging techniques and histology compare with one another in participants that performed 10 weeks of resistance training. Our suggests results show poor agreement yielded from other common techniques, researchers should be aware this limitation.
Aging is the primary risk factor for development of many chronic diseases, including dementia, cardiovascular disease, and diabetes. There significant interest in identifying novel “geroprotective” agents, by repurposing existing drugs, but such treatments may affect organ systems differently. One current example nucleoside reverse transcriptase inhibitor 3TC, which has been increasingly studied as a potential gerotherapeutic. Recent data suggest 3TC reduce inflammation improve cognitive...
Resistance training generally increases skeletal muscle hypertrophy, whereas aging is associated with a loss in mass. Interestingly, select studies suggest that aging, as well resistance training, may lead to reduction the abundance of myofibrillar (or contractile) protein (per mg tissue). Proteomic interrogations have also demonstrated weeks months appreciable alterations proteome. Given this evidence, purpose small pilot study was examine total sarcoplasmic concentrations wet muscle) from...
Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously MF isolation methods with intent determining which method was most suitable protein and solubilization. Here, we provide an optimized protocol to isolate sarcoplasmic solubilized fractions mammalian muscle downstream assays.
While high-load resistance training increases muscle hypertrophy, the intramuscular protein responses to this form of remains largely unknown. In current study, recreationally resistance-trained college-aged males (N=15; mean±SD: 23±3 years old, 6±5 training) performed full-body, low-volume, (68%-90% one repetition maximum (1RM)) over 10 weeks. Back squat strength testing, body composition and a vastus lateralis biopsy were before (PRE) 72 hours after 10-week program (POST). Fiber...
The aim of this study was to investigate the effects resistance training (RT) on redox status skeletal muscle in older adults. Thirteen males aged 64 ± 9 years performed full-body RT 2x/week for 6 weeks. Muscle biopsies were obtained from vastus lateralis prior and following RT. mRNA, protein, enzymatic activity levels various endogenous antioxidants determined. In addition, 4-hydroxynonenal protein carbonyls determined as markers oxidative damage. Protein heat shock proteins (HSPs) also...
The bacteria inhabiting the gastrointestinal tract contribute to numerous host functions and can be altered by lifestyle factors. We aimed determine whether a 6-week training intervention fecal microbiome diversity and/or function in older males. Fecal samples were collected prior following twice-weekly supervised resistance 14 Caucasian males (65 ± 10 years, 28.5 3.2 kg/m2) with minimal experience. Participants randomized receive daily defatted peanut powder supplement providing 30 g...
The long interspersed nuclear element-1 (L1) is a retrotransposon that constitutes 17% of the human genome and associated with various diseases aging. Estimates suggest ~100 L1 copies are capable copying pasting into other regions genome. Herein, we examined if skeletal muscle markers affected by aging or an acute bout cycling exercise in humans. Apparently healthy younger (23 ± 3 y, n = 15) older participants (58 8 donated vastus lateralis biopsy before 1 h (PRE) at ~70% heart rate reserve....