A5045881517- Bone health and treatments
- Bone Metabolism and Diseases
- Epigenetics and DNA Methylation
- Parathyroid Disorders and Treatments
- Cancer-related gene regulation
- Sarcoma Diagnosis and Treatment
- Uterine Myomas and Treatments
- NF-κB Signaling Pathways
- TGF-β signaling in diseases
- Gastrointestinal Tumor Research and Treatment
- Metastasis and carcinoma case studies
- Ovarian cancer diagnosis and treatment
- Medical Imaging and Pathology Studies
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Testicular diseases and treatments
- Gastrointestinal disorders and treatments
- Soft tissue tumor case studies
- Hedgehog Signaling Pathway Studies
- Lymphoma Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Urinary and Genital Oncology Studies
- Advanced Glycation End Products research
- Fibroblast Growth Factor Research
Ehime University
2016-2025
Ehime University Hospital
2014-2023
Kobe University
2008-2022
Saiseikai Matsuyama Hospital
2018
Malmö University
2010
Lund University
2010
University of Southampton
2010
Heidelberg Repatriation Hospital
2010
Kyoto University
2007
Washington University in St. Louis
1995-2001
White adipocytes are unique in that they contain large unilocular lipid droplets occupy most of the cytoplasm. To identify genes involved maintenance mature adipocytes, we expressed dominant-negative PPARgamma 3T3-L1 cells and performed a microarray screen. The fat-specific protein 27 kDa (FSP27) was strongly downregulated this context. FSP27 expression correlated with induction differentiation cultured preadipocytes, localized to murine white vivo. Ablation mice resulted formation...
To explore a novel adipokine, we screened adipocyte differentiation‐related gene and found that TIG2/chemerin was strongly induced during the differentiation. Chemerin secreted by mature 3T3‐L1 adipocytes expressed abundantly in adipose tissue vivo as recently described. Intriguingly, expression of chemerin differently regulated liver db / mice. In addition, serum concentration decreased its receptor/ChemR23 were adipocytes, suggesting function autocrine/paracrine fashion. Finally,...
Diabetic nephropathy is a major risk of end-stage kidney disease. Many complex factors relate to the progression diabetic nephropathy. Using nonobese type 2 diabetes model rats, we confirmed that oxidative stress was crucial factor. Because recent studies suggest vitamin D could suppress stress, explored whether active analog, maxacalcitol, also attenuate and prevent rats aged 20 weeks were divided into 3 groups treated with insulin, vehicle. At age 30 weeks, blood urine analyses, renal...
Breast cancer metastasis to bone is a multistep process requiring attachment of tumor cells the and marrow environment. The precise adhesion molecules involved in skeletal homing breast are unknown but likely include integrins. We investigated expression vitronectin receptor (alpha V beta 3) by residing because this heterodimer mediates osteoclast-bone recognition. used immunohistochemistry situ hybridization systematic study 22 biopsies containing metastases available samples corresponding...
ABSTRACT We previously reported that cells harboring the hepatitis C virus (HCV) RNA replicon as well those expressing HCV NS3/4A exhibited increased sensitivity to suboptimal doses of apoptotic stimuli undergo mitochondrion-mediated apoptosis (Y. Nomura-Takigawa, et al., J. Gen. Virol. 87:1935-1945, 2006). Little is known, however, about whether or not infection induces virus-infected cells. In this study, by using chimeric J6/JFH1 strain genotype 2a, we demonstrated induced cell death in...
Liver dysfunction in adult hypopituitary patients with GH deficiency (GHD) has been reported and an increased prevalence of nonalcoholic fatty liver disease (NAFLD) suggested. The objective the present study was to elucidate pathophysiology GHD. We recruited 69 consecutive Japanese GHD examined NAFLD by ultrasonography steatohepatitis (NASH) biopsy. Patients had given routine replacement therapy except for GH. compared these healthy age-, gender-, BMI-matched controls. further analyzed...
Although various function of chemerin have been suggested, its physiological role remains to be elucidated. Here we show that chemerin-deficient mice are glucose intolerant irrespective exhibiting reduced macrophage accumulation in adipose tissue. The intolerance was mainly due increased hepatic production and impaired insulin secretion. Chemerin receptor ChemR23 were expressed β-cell. Studies using isolated islets perfused pancreas revealed glucose-dependent secretion (GSIS) mice....
Chronic kidney disease (CKD) is an important risk factor for cardiovascular (CVD). Increased oxidative stress plays a role in the pathogenesis of CVD CKD patients. The oral charcoal adsorbent AST-120 attenuates progression possibly by removing uraemic toxins such as indoxyl sulfate (IS), and reduces stress. We investigated relationship between cardiac damage its prevention AST-120.Male Lewis rats were administered adriamycin at 8 weeks age, right was removed 12 age. From 14 treated daily...
To evaluate the significance of alterations in DNA methylation during multistage carcinogenesis pancreas, tissue samples 13 peripheral pancreatic duct epithelia showing no remarkable histological changes without inflammatory background (DE), 20 with (DEI), 40 intraepithelial neoplasias (PanIN) and 147 areas ductal carcinoma were microdissected from surgically resected specimens 58 patients embedded into agarose beads. The subjected to methylation-specific PCR (MSP) status p14, p15, p16, p73,...
Abstract Receptor activator of NF‐κB ligand (RANKL) has been identified as requisite for osteoclastogenesis. To elucidate the molecular mechanism that conducts its catabolic action on bone, effect 1α,25 dihydroxyvitamin D 3 (1α,25(OH) 2 ) osteoclastogenesis and RANKL mRNA expression was examined by coculture, RT‐PCR nuclear run‐on studies. By accelerating transcription rate gene in SaOS2 osteoblastic cells, 1α,25(OH) enhanced vitro osteoclast formation from peripheral monocytes. Cloning...
Abstract Breast cancer is frequently associated with osteolytic bone metastasis, where osteoclasts play a major role in destruction. Recently, osteoclast differentiation factor (RANKL) has been identified as prerequisite for the formation and maintenance of from haematopoietic precursors. To elucidate mechanism osteoclastogenesis destruction bone‐residing breast cancer, PTHrP‐producing (MCF‐7) ‐non‐producing (MCF‐7UP) human cells were subcutaneously injected into forehead nude mice...
Abstract Testicular germ cell tumors of adolescents and adults (TGCTs) can be classified into seminomatous nonseminomatous tumors. Various lines, predominantly embryonal carcinoma, have been established proven to valuable for pathobiological clinical studies. So far, no lines derived from seminoma which constitutes more than 50% invasive TGCTs. Such a line is essential experimental investigation biological characteristics the origin TGCTs, i.e., carcinoma in situ testis, shows cell. Before...
We examined whether the serum matrix metalloproteinase-2 (MMP-2) level and MMP-2 density could be predictors of development extension prostate cancer. Serum samples were collected before any clinical treatment from 98 patients with cancer 76 benign prostatic hyperplasia (BPH). Control sera obtained 70 healthy men. The was determined by 1-step enzyme immunoassay. A newly defined parameter dividing volume, which measured ultrasonography. mean in significantly higher than control BPH groups....
Non-structural protein 4A (NS4A) of Hepatitis C virus (HCV) functions as a cofactor for NS3 by forming complex with it to augment its enzymic activities. NS4A also forms other HCV proteins, such NS4B/NS5A, facilitate the formation viral RNA replication on endoplasmic reticulum (ER) membrane. In addition essential role in replication, is thought be involved pathogenesis affecting cellular functions. this study, was demonstrated that localized not only ER, but mitochondria when expressed...
The Rho family GTPase Rac1 has been implicated in the regulation of glucose uptake myoblast cell lines. However, no evidence for role provided by a mouse model. purpose this study is to test involvement insulin action skeletal muscle. Intravenous administration indeed elicited activation gastrocnemius muscle, suggesting signaling pathway. We then examined whether insulin-stimulated translocation facilitative transporter GLUT4 from its storage sites muscle sarcolemma depends on Rac1. show...
Abstract Increased bone fragility attributed to osteopenia is a serious side effect of glucocorticoid treatment. Glucocorticoid‐induced loss caused primarily by hypofunction and apoptosis osteoblasts, secondarily accelerated resorption. To explore the mechanism whereby dexamethasone (Dex) stimulates osteoclastogenesis in coculture system, we analyzed Dex on expression both mouse osteoprotegerin (OPG) receptor activator NF‐κB ligand (RANKL). reduced OPG transcripts protein secretion ST2...
Apc Min/+ mice, carrying an inactivated allele of the a denomatous polyposis coli gene, are widely used as animal model for human colorectal tumorigenesis, where tumor environment, such inflammation, is known to play critical role in progression. We previously demonstrated that phospholipase C (PLC)ε, effector Ras and Rap small GTPases, plays crucial two-stage skin chemical carcinogenesis using 12- O -tetradecanoyl-phorbor-13-acetate (TPA) promoter through augmentation TPA-induced...