A5012567466- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Radiopharmaceutical Chemistry and Applications
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Brain Metastases and Treatment
- Drug Transport and Resistance Mechanisms
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Medical Imaging and Pathology Studies
- Glioma Diagnosis and Treatment
- Photoacoustic and Ultrasonic Imaging
- Sarcoma Diagnosis and Treatment
- Lung Cancer Research Studies
- Immune cells in cancer
- Medical Imaging Techniques and Applications
- Ubiquitin and proteasome pathways
- Fish biology, ecology, and behavior
- CAR-T cell therapy research
University of Coimbra
2016-2025
Universidade Federal do Maranhão
2022
University College London
2022
Brigham and Women's Hospital
2021
Center for Innovation
2019
Centro de Neurociências e Biologia Celular
2019
Association for Innovation and Biomedical Research on Light and Image
2011-2018
University of South Carolina
2015
Leiden University Medical Center
2006-2007
University of Aveiro
1995
Small extracellular vesicles (SEVs) offer a promising strategy for tissue regeneration, yet their short lifetime at the injured limits efficacy. Here, we show that kinetics of SEV delivery impacts regeneration tissue, cellular, and molecular levels. We multiple carefully timed applications SEVs had superior than single dose same total concentration SEVs. Importantly, diabetic non-diabetic wounds treated with time point an injectable light-triggerable hydrogel containing demonstrated robust...
Abstract Protein kinase C (PKC) isozymes play major roles in human diseases, including cancer. Yet, the poor understanding of isozymes-specific functions and limited availability selective pharmacological modulators PKC have clinical translation PKC-targeting agents. Here, we report first small-molecule PKCδ-selective activator, 7 α -acetoxy-6 β -benzoyloxy-12- O -benzoylroyleanone (Roy-Bz), which binds to PKCδ-C1-domain. Roy-Bz potently inhibited proliferation colon cancer cells by inducing...
Abstract Background Osteosarcoma is a bone-forming tumor of mesenchymal origin that presents clinical pattern consistent with the cancer stem cell model. Cells stem-like properties (CSCs) have been identified in several tumors and hypothesized as responsible for relative resistance to therapy relapses. In this study, we aimed identify characterize CSCs populations human osteosarcoma line explore their role responsiveness conventional therapies. Methods were isolated from MNNG/HOS using...
Extracellular vesicles (EVs) are major conveyors of biological information, mediating local and systemic cell‐to‐cell communication under physiological pathological conditions. These endogenous have been recognized as prominent drug delivery vehicles several therapeutic cargoes, including doxorubicin (dox), presenting advantages over the classical approaches. Although dox is one most effective anti‐tumour agents in clinical practice, its use very often hindered by consequent dramatic...
Although intra-axonal protein synthesis is well recognized in cultured neurons and during development <i>in vivo</i>, there have been few reports of mRNA localization and/or translation mature CNS axons. Indeed, previous work indicated that axons contain much lower quantities translational machinery than PNS axons, leading to the conclusion capacity for linked intrinsic a neuron regeneration, with showing less growth after injury neurons. However, when regeneration by facilitated, it not...
Osteosarcoma is a bone tumor, displaying significant cellular and histological heterogeneity complex genetic phenotype. Although multiple studies strongly suggest the presence of cancer stem cells in osteosarcoma, consensus on their characterization still missing. We used combination functional assays (sphere-forming, Aldefluor, side-population) for identification cell populations osteosarcoma lines. Expression stemness-related transcription factors, quiescent nature, vivo tumorigenicity,...
Machado-Joseph disease (MJD)/spinocerebellar ataxia type 3 (SCA3) is the most common autosomal dominantly inherited worldwide. It caused by an over-repetition of trinucleotide CAG within ATXN3 gene, which confers toxic properties to ataxin-3 (ATXN3) species. RNA interference technology has shown promising therapeutic outcomes but still lacks a non-invasive delivery method brain. Extracellular vesicles (EVs) emerged as vehicles due their capacity deliver small nucleic acids, such microRNAs...
Brain metastasis (BrM) is a devastating end-stage neurological complication that occurs in up to 50% of human epidermal growth factor receptor 2-positive (HER2+) breast cancer (BC) patients. Understanding how disseminating tumor cells manage cross the blood-brain barrier (BBB) essential for developing effective preventive strategies. We identified ecto-nucleotidase ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1) as specifically enriched secretome HER2+ brain metastatic cells,...