Summary The cellular and subcellular distributions of trace elements can provide important clues to understanding how the are transported stored in plant cells, but mapping their is a challenging task. arsenic, iron, zinc, manganese copper, as well physiologically related macro‐elements, were mapped node, internode leaf sheath rice ( Oryza sativa ) using synchrotron X‐ray fluorescence (S‐XRF) high‐resolution secondary ion mass spectrometry (NanoSIMS). Although copper silicon generally showed...

The cytoskeleton is an early attribute of cellular life, and its main components are composed conserved proteins. actin has a direct impact on the control cell size in animal cells, but mechanistic contribution to growth plants remains largely elusive. Here, we reveal role regulating plants. shows proximity vacuoles, phytohormone auxin not only controls organization filaments also impacts vacuolar morphogenesis actin-dependent manner. Pharmacological genetic interference with actin-myosin...

Significance A vertebrate photoreceptor cell depends on the elaboration of its cilium to generate light-sensitive organelle, outer segment (OS), which is made up a stack membrane disks, containing visual receptor, opsin. How this occurs has been subject recent controversy. Here we used electron microscope tomography obtain 3D analysis organization at base OS, where new disks are continually replace older ones. We show that nascent disk continuous with ciliary plasma membrane, and appears...

Adult mouse articular cartilage (AC) has not been thoroughly described using high resolution imaging techniques, despite the fact that availability of knockout mice with specific extracellular matrix (ECM) mutations have renewed interest in as a model for variety different human conditions. With osteoarthritis affecting millions people worldwide, investigations into structure and, therefore, ability AC to act load-bearing tissue, are crucial developing treatments and prevention techniques...

Increased failure rates due to metallic wear particle-associated adverse local tissue reactions (ALTR) is a significant clinical problem in resurfacing and total hip arthroplasty. Retrieved periprosthetic of 53 cases with corrosion/conventional particles from 285 revision operations for ALTR was selected nano-analyses. Three major classes implants associated ALTR, metal-on-metal arthroplasty (MoM HRA) large head replacement LHTHA) non-metal-on-metal dual modular neck (Non-MoM DMNTHA) were...

The mixture of different metallic nanoparticles released from intended and unintended wearing orthopaedic implants such as the Co/Cr cup head, sleeves or tapers their interface with Ti stems in case hip prostheses are a leading cause adverse inflammatory responses cytotoxicity to host.This study assessed vitro cytotoxic effects three (Co, Cr Ti) separately combination on macrophages. vivo were also evaluated after peri-tibial soft tissue injection mice.The results demonstrated that Co, Cr,...

Flagellum motility is critical for normal human development and transmission of pathogenic protozoa that cause tremendous suffering worldwide. Biophysical principles underlying eukaryotic flagella are conserved from protists to vertebrates. However, individual cells exhibit diverse waveforms depend on cell-specific elaborations basic flagellum architecture. Trypanosoma brucei a uniflagellated protozoan parasite causes African sleeping sickness. The T. comprised 9+2 axoneme an extra-axonemal...

The major mammalian bloodstream form of the African sleeping sickness parasite Trypanosoma brucei multiplies rapidly, and it is important to understand how these cells divide. Organelle inheritance involves complex spatiotemporal re-arrangements ensure correct distribution daughter cells. Here, serial block face scanning electron microscopy (SBF-SEM) was used reconstruct whole individual at different stages cell cycle give an unprecedented temporal, spatial quantitative view organelle...

Flagella are highly conserved organelles present in a wide variety of species. In Trypanosoma brucei the single flagellum is necessary for morphogenesis, cell motility and pathogenesis, attached along body. A new formed alongside old during division cycle. (insect) procyclic form, flagella connector (FC) attaches tip to side flagellum, ensuring faithful replication architecture. The FC not bloodstream form parasite. We show here, using imaging techniques including serial block-face scanning...

Abstract We report the case of a 78‐year‐old man with Helicobacter pylori infection and three primary neoplasms stomach: adenocarcinoma, mucosa‐associated lymphoid tissue (MALT) lymphoma gastrointestinal stromal cell tumor. is associated gastric carcinoma MALT lymphoma, although their simultaneous occurrence rare. Gastrointestinal tumors have not been to date. This appears be first synchronous these tumors. © 2002 Blackwell Publishing Asia Pty Ltd

purpose. To investigate the expression of harmonin in mouse retina, test for ultrastructural and physiological mutant phenotypes retina an Ush1c mouse, define detail retinal phenotype human USH1C. methods. Antibodies were generated against harmonin. Harmonin isoform distribution was examined by Western blot analysis immunocytochemistry. Retinas deaf circler (dfcr) mice, which possess Ush1c, analyzed microscopy electroretinography (ERG). Two siblings with homozygous 238_239insC (R80fs) USH1C...

Reticulons are integral ER membrane proteins characterised by a reticulon homology domain comprising four transmembrane domains which results in the sitting W-topology. Here we report on novel subgroup of reticulons with an extended N-terminal and particular arabidopsis 20. Using high resolution confocal microscopy show that 20 is located unique punctate pattern membrane. Its closest homologue 19 labels whole ER. Other than demonstrated for other members protein family RTN20 do not display...

Breast cancer is the most commonly occurring in women worldwide and second common overall. The development of new therapies to treat this devastating malignancy needed urgently. Nanoparticles are one class nanomaterial with multiple applications medicine, ranging from their use as drug delivery systems promotion changes cell morphology control gene transcription. made natural polymer chitosan easy produce, have a very low immunogenic profile, diffuse easily into cells. One hallmark feature...

P10 is a small, abundant baculovirus protein that accumulates to high levels in the very late stages of infection cycle. It associated with number intracellular structures and implicated diverse processes from occlusion body maturation nuclear stability lysis. However, studies have also shown it non-essential for virus replication, at least cell culture. Here, we describe use serial block-face scanning electron microscopy achieve high-resolution 3D characterisation within Trichoplusia ni...

Summary Up‐to‐date imaging approaches were used to address the spatiotemporal organisation of endomembrane system in secretory cells Dionaea muscipula . Different ‘slice and view’ methodologies performed on resin‐embedded samples finally achieve a 3D reconstruction cell architecture, using ultrastructural tomography, array serial block face‐scanning electron microscopy (SBF‐SEM), correlation, volume rendering at light level. Observations cryo‐fixed by high‐pressure freezing revealed changes...