A5029644665- Antimicrobial Resistance in Staphylococcus
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Ocular Infections and Treatments
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- HIV/AIDS drug development and treatment
- Ocular Surface and Contact Lens
- Genomics and Phylogenetic Studies
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Antifungal resistance and susceptibility
- Antimicrobial Peptides and Activities
University of Sheffield
2020-2024
Coronaviruses such as SARS-CoV-2, which is responsible for COVID-19, depend on virus spike protein binding to host cell receptors cause infection. The SARS-CoV-2 binds primarily ACE2 target cells and then processed by membrane proteases, including TMPRSS2, leading viral internalisation or fusion with the plasma membrane. It has been suggested, however, that other than may be involved in binding. We have investigated interactions of recombinant versions human epithelial lines express low/very...
The clinical burden of infections caused by antimicrobial resistant (AMR) pathogens is a leading threat to public health. Maintaining the effectiveness existing drugs or finding ways reintroduce which resistance widespread an important part efforts address AMR crisis.
Abstract The SARS-CoV-2 spike protein is known to bind the receptor, ACE2, on surface of target cells. processed by membrane proteases, including TMPRSS2, and either internalises or fuses directly with cell, leading infection. We have identified a human cell line that expresses both ACE2 RT4 urinary bladder transitional carcinoma, used it develop proxy assay for viral interactions host A tagged recombinant form protein, containing S1 S2 domains, interacted strongly cells as determined flow...
Staphylococcus aureus is an opportunistic bacterial pathogen that often results in difficult-to-treat infections. One mechanism used by S. to enhance survival during infection the stringent response. This a stress pathway utilizes nucleotides (p)ppGpp reallocate resources, shutting down growth until conditions improve. Small colony variants (SCVs) of are frequently associated with chronic infections, and this phenotype has previously been linked hyperactive Here, we examine role long-term...
Epithelial colonization is a critical first step in bacterial pathogenesis.
Abstract Objectives Epithelial colonisation is a critical first step in bacterial pathogenesis. Staphylococcus aureus can utilise several host factors to associate with cells, including α5βl integrin and heparan sulphate proteoglycans, such as the syndecans. Here, we demonstrate that partner protein of both integrins syndecans, membrane adapter tetraspanin CD9, essential for syndecan-mediated staphylococcal adhesion. Fibronectin also this process while are only post-adhesion entry into human...
Abstract Elucidating the complex mechanisms controlling mecA /PBP2a-mediated β-lactam resistance in methicillin resistant Staphylococcus aureus (MRSA) has potential to identify new drug targets with therapeutic potential. Here, we report that mutations interfere de novo purine synthesis ( pur operon), transport (NupG, PbuG and PbuX) nucleotide salvage pathway (DeoD2, Hpt) increased MRSA strain JE2. Extrapolating from these findings, exogenous guanosine xanthosine, which are fluxed through...
Abstract Staphylococcus aureus is a bacterial pathogen that poses major threat to human health. The ability of this bacterium adapt stresses encountered in the host essential for disease. stringent response signalling pathway utilised by all bacteria alarm cells when stressed, and has been linked virulence number species. This controlled nucleotide alarmones guanosine tetra-(ppGpp) pentaphosphate (pppGpp: collectively termed (p)ppGpp), produced S. three synthetase enzymes: Rel, RelP RelQ....