A5048725428- Complement system in diseases
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Adenosine and Purinergic Signaling
- Platelet Disorders and Treatments
- Neuropeptides and Animal Physiology
- Ocular Infections and Treatments
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Chemokine receptors and signaling
- Bacterial biofilms and quorum sensing
- Amyotrophic Lateral Sclerosis Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Caveolin-1 and cellular processes
- Blood Coagulation and Thrombosis Mechanisms
- Antimicrobial Peptides and Activities
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- RNA Interference and Gene Delivery
- Neurogenetic and Muscular Disorders Research
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Pneumonia and Respiratory Infections
- Inhalation and Respiratory Drug Delivery
University of Sheffield
2016-2025
Immunité et Cancer
2025
Thai Red Cross Society
2024
Christian Medical College & Hospital
2024
Christian Medical College
2024
Chulalongkorn University
2024
King Chulalongkorn Memorial Hospital
2024
Medizinische Hochschule Hannover
1999-2013
Universidad Complutense de Madrid
2004-2005
Universidad Autónoma de Madrid
2004-2005
ABSTRACT A truncated soluble form of the hepatitis C virus E2 glycoprotein, 661 , binds specifically to surface cells expressing human CD81 (hCD81) but not other members tetraspanin family (CD9, CD63, and CD151). No differences were noted between level binding hCD81 expressed on rat RBL or KM3 compared Daudi Molt-4 cells, suggesting that additional human-cell-specific factors are required for primary interaction with cell surface. did interact African green monkey (AGM) COS which differs...
The NLRP3 inflammasome controls interleukin-1β maturation in antigen-presenting cells, but a direct role for human adaptive immune cells has not been described. We found that the assembles CD4(+) T and initiates caspase-1-dependent secretion, thereby promoting interferon-γ production helper 1 (T(H)1) differentiation an autocrine fashion. assembly requires intracellular C5 activation stimulation of C5a receptor (C5aR1), which is negatively regulated by surface-expressed C5aR2. Aberrant...
CD81 has been described as a putative receptor for hepatitis C virus (HCV); however, its role in HCV cell entry not characterized due to the lack of an efficient culture system. We have examined glycoprotein-dependent by using recently developed retroviral pseudotyping Human immunodeficiency (HIV) pseudotypes bearing E1E2 glycoproteins show restricted tropism human liver lines. Although all permissive lines express CD81, expression alone is sufficient allow viral entry. required HIV-HCV...
The substantial variations in the responses of cells to anaphylatoxin C5a and its desarginated form, C5adR74, suggest that more than one type cell surface receptor for these ligands might exist. However, only a single CD88, has been characterized date. Here we report orphan C5L2/gpr77, which shares 35% amino acid identity with binds high affinity but 10-fold higher C5adR74 CD88. C5L2 also moderate C3a, cross-competition studies C3a distinct site from C5a. C4a was able displace suggesting...
ABSTRACT Human CD81 has been previously identified as the putative receptor for hepatitis C virus envelope glycoprotein E2. The large extracellular loop (LEL) of human differs in four amino acid residues from that African green monkey (AGM), which does not bind We mutated each positions to corresponding AGM and expressed them soluble fusion LEL proteins bacteria or complete membrane mammalian cells. found 186 be critical interaction with viral glycoprotein. This residue was also important...
VCAM-1 and ICAM-1, receptors for leukocyte integrins, are recruited to cell–cell contact sites on the apical membrane of activated endothelial cells. In this study, we show that recruitment is independent ligand engagement, actin cytoskeleton anchorage, heterodimer formation. Instead, ICAM-1 by inclusion within specialized preformed tetraspanin-enriched microdomains, which act as adhesive platforms (EAPs). Using advanced analytical fluorescence techniques, have characterized diffusion...
Abstract Complement activation products are elevated in the cerebrospinal fluid and spinal cord of patients with amyotrophic lateral sclerosis (ALS). In this study, we demonstrate complement system involvement a rodent model ALS (human SOD1G93A transgenic rats). With end-stage disease, rats displayed marked deposition C3/C3b, significant up-regulation C5aR lumbar cord. This was associated increased numbers C5aR-positive astrocytes. However, expression C5L2, alternative receptor for C5a,...
Coronaviruses such as SARS-CoV-2, which is responsible for COVID-19, depend on virus spike protein binding to host cell receptors cause infection. The SARS-CoV-2 binds primarily ACE2 target cells and then processed by membrane proteases, including TMPRSS2, leading viral internalisation or fusion with the plasma membrane. It has been suggested, however, that other than may be involved in binding. We have investigated interactions of recombinant versions human epithelial lines express low/very...
Pseudomonas aeruginosa infections in the lungs affect millions of children and adults worldwide. To our knowledge, no clinically validated prognostic biomarkers for chronic pulmonary P. exist. Therefore, this study aims to identify potential markers biofilm lung infections. Here, we screened expression 11 regulatory genes (tesG, algD, lasR, lasA, lasB, pelB, phzF, rhlA, rsmY, rsmZ, sagS) associations between clinical status infection. RNA was extracted from 210 sputum samples patients (n =...
The orphan receptor C5L2 has recently been described as a high affinity binding protein for complement fragments C5a and C3a that, unlike the previously (CD88), couples only weakly to G(i)-like G proteins (Cain, S. A., Monk, P. N. (2002) J. Biol. Chem. 277, 7165-7169). Here we demonstrate that binds metabolites of C4a C3a, des-Arg(77), des-Arg(77) (also known acylation-stimulating or ASP) at site distinct from site. these does not stimulate degranulation transfected rat basophilic leukemia...
The complement system is thought to be involved in the pathogenesis of numerous neurological diseases, although its precise role remains controversial. In this study we used orally active C5a receptor antagonists (PMX53 and PMX205) developed our laboratories a rat model 3-nitropropionic acid (3-NP) -induced Huntington's disease. Administration (10 mg/kg/day, oral) either 48 h pre- or post-toxin significantly reduced body weight loss, anorexia, behavioral motor deficits associated with 3-NP...
C5L2 is a 7 transmembrane domain receptor for complement fragment C5a that, unlike the classical receptor, C5aR, does not couple to G proteins. However, in mice where has been deleted, response altered, suggesting that may have signaling function. In order investigate whether human also some capacity transduce signals, we attempted produce competent form of by inserting C5aR sequences at three key protein activation motifs. detected neither an intracellular Ca(2+) nor beta-arrestin...
C5a receptors are found in the central nervous system (CNS), on both neurons and glia. However, origin of C5a, which activates these receptors, is unclear. In present study, we show that primary cultured mouse cortical constitutively express C5, precursor classical receptor for CD88. With cell ischemia caused by 12 h glucose deprivation, or oxygen-glucose deprivation (OGD), demonstrated increased apoptosis, up-regulation CD88, levels media. Exogenous murine (100 nM) added to neuronal...
During experimental sepsis, excessive generation of the anaphylatoxin C5a results in reduction receptor (C5aR) on neutrophils. These events have been shown to result impaired innate immunity. However, regulation and fate C5aR neutrophils during sepsis are largely unknown. In contrast 30 healthy volunteers, 60 patients septic shock presented evidence complement activation with significantly increased serum levels C3a, C5a, C5b-9. group, corresponding decrease hemolytic activity distinguished...