A5083143645- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Prostate Cancer Treatment and Research
- Peptidase Inhibition and Analysis
- Advanced Proteomics Techniques and Applications
- MicroRNA in disease regulation
- Mass Spectrometry Techniques and Applications
- Ubiquitin and proteasome pathways
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Multiple Myeloma Research and Treatments
- Glycosylation and Glycoproteins Research
- Protein Degradation and Inhibitors
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- ATP Synthase and ATPases Research
- Cancer, Lipids, and Metabolism
- Hemoglobinopathies and Related Disorders
Cancer Institute (WIA)
2013-2024
Molecular Oncology (United States)
2015-2020
The University of Texas MD Anderson Cancer Center
2008-2012
University of Leicester
2012
Medizinische Hochschule Hannover
2012
University of Miami
2005-2009
Sylvester Comprehensive Cancer Center
2005-2009
Miami VA Healthcare System
2007
Cervical cancer is the most common among Indian women. This has well defined pre-cancerous stages and evolves over 10-15 years or more. study was undertaken to identify differentially expressed genes between normal, dysplastic invasive cervical cancer. A total of 28 cancers, 4 CIN3/CIS, CIN1/CIN2 5 Normal cervix samples were studied. We have used microarray technique followed by validation significant relative quantitation using Taqman Low Density Array Real Time PCR. Immunohistochemistry...
Abstract Glioblastoma is the most frequent and malignant brain tumor, characterized by an elevated capacity for cellular proliferation invasion. Recently, it was demonstrated that podoplanin membrane sialo‐glycoprotein encoded PDPN gene over‐expressed related to invasion in astrocytic tumors; however mechanisms of regulation are still unknown. MicroRNAs noncoding RNAs regulate expression several biological processes diseases, including cancer. Nevertheless, their roles invasion,...
Gastric cancer is one of the common cancers seen in south India. Unfortunately more than 90% are advanced by time they report to a tertiary centre country. There an urgent need characterize these and try identify potential biomarkers novel therapeutic targets.We used 24 gastric cancers, 20 Paired normal (PN) 5 apparently tissues obtained from patients with non-gastric (Apparently - AN) for microarray study followed validation significant genes (n = 63) relative quantitation using Taqman Low...
Abstract Background Transcriptional silencing associated with aberrant promoter methylation has been established as an alternate pathway for the development of cancer by inactivating tumor suppressor genes. TMS1 (Target Methylation induced Silencing), also known ASC (Apoptosis Speck like protein containing a CARD) is gene which encodes CARD (caspase recruitment domain) regulatory and shown to promote apoptosis directly activation downstream caspases. This study describes TMS1/ASC in prostate...
Abstract Defects in apoptotic pathway contribute to uncontrolled proliferation of cancer cells and confer resistance chemotherapy. Growth arrest DNA damage inducible, alpha (GADD45α) is up-regulated on docetaxel treatment may docetaxel-mediated cytotoxicity. We examined the mechanism regulation GADD45α prostate effect its up-regulation sensitivity Expression PC3 was higher than that Du145 LNCaP (17- 12-fold, respectively; P < 0.05). Although proximal promoter region unmethylated all...
Abstract Breast cancer is the most common malignancy among women globally. Development of a reliable plasma biomarker panel might serve as non-invasive and cost-effective means for population-based screening disease. Transcriptomic profiling breast tumour, paired normal apparently tissues, followed by validation shortlisted genes using TaqMan ® Low density arrays Quantitative real-time PCR was performed in South Asian women. Fifteen candidate protein markers 3 epigenetic were validated first...
Based on our previous report gastric cancer which documented ATP4A and ATP4B mRNA down-regulation in tumors relative to normal tissues, we hypothesized that epigenetic mechanisms could be responsible. expression cell lines AGS, SNU638 NUGC-3 was examined using reverse transcriptase PCR (RT-PCR). AGS cells were treated with TSA or 5'-AzaDC methylation specific (MSP) bisulfite sequencing (BSP) analysis performed. MSP DNA from paraffin embedded tissues sections plasma. Expression revealed...
A combination of 5-fluorouracil (FU), epirubicin (EP) and cyclophosphamide (CP) is routinely employed in the treatment breast cancer. The objective this study was to develop a reverse phase high-performance liquid chromatography (HPLC)-UV method for simultaneous quantitative analysis triple-drug their metabolites plasma. RP-HPLC system with C18 column diode array detector employed. plasma samples were precipitated acetonitrile supernatant dried under flow nitrogen gas. mobile comprised two...
Purpose: To find the radiation sensitivity of human cervical carcinoma cell lines and to investigate effect dominant negative-Ubiquitin-conjugating enzyme E2C (DN-UBE2C) on proliferation response.Materials methods: Radiation sensitivities (SiHa, HeLa, BU25TK, ME 180, C33A) were analyzed by assessing their survival after irradiation MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Soft agar cloning assay, growth curve response DN-UBE2C...
The insulin/IGF-1R/PI3K/Akt signalling cascade is increasingly being linked to breast cancer development, with aldose reductase (AR) playing a key role in mediating the crosstalk between this pathway and angiogenesis. current study was designed investigate whether nimbolide, neem limonoid, targets oncogenic signaling network prevent angiogenesis cancer. Breast cells (MCF-7, MDA-MB-231), EAhy926 endothelial cells, MDA-MB-231 xenografted nude mice, tumour tissues from patients were used for...
Mitochondrial ribosomal small subunit (MRPS) group of proteins is structural constituents the mitoribosomes involved in translation. Recent studies indicate role tumourigenic process, however, unlike cytosolic proteins, knowledge on MRPS alternate cellular processes very limited. Mapping protein-protein interactions (PPIs) onto known can be a valuable tool to identify novel protein functions. In this study, PPIs we have constructed 31 glutathione-S-transferase (GST)/MRPS fusion clones....
Objective: To differentiate plasma from ovarian cancer and healthy individuals using MALDI-TOF mass spectroscopy.Materials methods: was used to generate profiles of immuno-depleted samples (89 cancers 199 individuals) that were fractionated three types magnetic beads (HIC8, WCX IMAC-Cu).Results: Differentially expressed ranges showing >1.5–2-fold change in expression HIC8 (30), (12) IMAC-Cu (6) fractions identified. Cross validation recognition capability scores for the models indicated...