A5006023626- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Amyotrophic Lateral Sclerosis Research
- RNA modifications and cancer
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Circular RNAs in diseases
- Insect and Pesticide Research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Advanced Glycation End Products research
- Prion Diseases and Protein Misfolding
- Bioinformatics and Genomic Networks
- RNA Interference and Gene Delivery
- Drug-Induced Hepatotoxicity and Protection
- Gene expression and cancer classification
- Biochemical Acid Research Studies
- Plant and animal studies
- Nerve injury and regeneration
- Prostate Cancer Treatment and Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Glaucoma and retinal disorders
- Parkinson's Disease Mechanisms and Treatments
Istituto di Farmacologia Traslazionale
2015-2025
National Research Council
2009-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2023
Fondazione Santa Lucia
2013-2023
Consorzio Roma Ricerche
2021
Einstein Medical Center Philadelphia
2021
University of Rome Tor Vergata
2013-2020
Università della Svizzera italiana
2019
Institute of Oncology Research
2016-2019
The University of Texas MD Anderson Cancer Center
2008-2018
MicroRNAs (miRNAs) are small, noncoding RNAs that can contribute to cancer development and progression by acting as oncogenes or tumor suppressor genes. Recent studies have also linked different sets of miRNAs metastasis through either the promotion suppression this malignant process. Interestingly, epigenetic silencing with features CpG island hypermethylation is emerging a common hallmark human tumors. Thus, we wondered whether there was miRNA profile characteristic metastasis. We used...
microRNAs (miRNAs) regulate expression by promoting degradation or repressing translation of target transcripts. miRNA sites have been catalogued in databases based on experimental validation and computational prediction using various algorithms. Several online resources provide collections multiple but need to be imported into other software, such as R, for processing, tabulation, graphing computation. Currently available site packages R are limited the number databases, types flexibility....
We studied miRNA profiles in 4419 human samples (3312 neoplastic, 1107 nonmalignant), corresponding to 50 normal tissues and 51 cancer types. The complexity of our database enabled us perform a detailed analysis microRNA (miRNA) activities. inferred genetic networks from expression cancer. also built, for the first time, specialized solid tumors leukemias. Nonmalignant displayed change hubs, most connected miRNAs. hsa-miR-103/106 were downgraded cancer, whereas hsa-miR-30 became prominent....
Background The physiopathology of sepsis continues to be poorly understood, and despite recent advances in its management, is still a life-threatening condition with poor outcome. If new diagnostic markers related pathogenesis will identified, specific therapies might developed mortality reduced. Small regulatory non-coding RNAs, microRNAs (miRNAs), were recently linked various diseases; the aim our prospective study was identify miRNAs that can differentiate patients early-stage from...
MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression at the posttranscriptional level and critical for many cellular pathways. The disruption of miRNAs their processing machineries also contributes to development human tumors. A common scenario miRNA in carcinogenesis is emerging shows impaired production and/or down-regulation these transcripts occurs neoplasms. Several lost have tumor-suppressor features, so strategies restore globally malignancies would be a welcome...
It is well known that bone mass density decreases with age. Age-related loss ascribed to several factors. Nonenzymatic glycation has been proposed as a new potential factor in the of during aging. In this study we evaluated concentration pentosidine, an advanced end product, cortical and trabecular plasma subjects undergoing orthopedic surgery. The relationship between these parameters clinical index osteoporosis was also studied. Samples 104 nondiabetic (74 women 30 men), 72 +/- 1 years...
Oncogenic activation of Bmi-1 is found in a wide variety epithelial malignancies including ovarian cancer, yet specific mechanism for overexpression has not been determined. Thus, realizing the immense pathologic significance we wanted to investigate if microRNA (miRNA) aberrations played role regulation cancer. In this report, identify two miRNAs, miR-15a and miR-16, that are underexpressed cell lines primary tissues. We show these miRNAs directly target 3' untranslated region significantly...
Although only 1.5% of the human genome appears to code for proteins, much effort in cancer research has been devoted this minimal fraction our DNA. However, last few years have witnessed realization that a large class non-coding RNAs (ncRNAs), named microRNAs, contribute development and progression by acting as oncogenes or tumor suppressor genes. Recent studies also shown epigenetic silencing microRNAs with features CpG island hypermethylation is common hallmark tumors. Thus, we wondered...
We investigated the expression of microRNAs (miRNAs) associated with replicative senescence in human primary keratinocytes. A cohort miRNAs up-regulated was identified by genome-wide miRNA profiling, and their change validated proliferative versus senescent cells. Among these, (miR)-138, -181a, -181b, -130b increased serial passages. miR-138, but not miR-130b, overexpression proliferating cells sufficient per se to induce senescence, as evaluated inhibition BrdU incorporation quantification...
Purpose: The oncogenic miR-155 is upregulated in many human cancers, and its expression increased more aggressive therapy-resistant tumors, but the molecular mechanisms underlying miR-155-induced therapy resistance are not fully understood. main objectives of this study were to determine role chemotherapy evaluate anti-miR-155 treatment chemosensitize tumors.Experimental Design: We performed vitro studies on cell lines investigate resistance. To assess effects inhibition chemoresistance, we...
Development of improved RNA interference-based strategies is utmost clinical importance. Although siRNA-mediated silencing EphA2, an ovarian cancer oncogene, results in reduction tumor growth, we present evidence that additional inhibition EphA2 by a microRNA (miRNA) further "boosts" its antitumor effects. We identified miR-520d-3p as suppressor upstream whose expression correlated with favorable outcomes two independent patient cohorts comprising 647 patients. Restoration prominently...
Alterations in glucose metabolism and epithelial-mesenchymal transition (EMT) constitute two important characteristics of carcinoma progression toward invasive cancer. Despite an extensive characterization each them separately, the links between EMT tumor cells remain elusive. Here we show that neuronal transporter GLUT3 contributes to uptake proliferation lung have undergone EMT. Using a panel human non-small cell cancer (NSCLC) lines, demonstrate is strongly expressed mesenchymal, but not...
MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate post-transcriptional gene expression. They influence a wide range of physiological functions, including neuronal processes, and regulated by various mechanisms, such as DNA methylation. This epigenetic mark is recognized transcriptional regulators the methyl CpG binding protein Mecp2. Rett syndrome complex neurological disorder has been associated with mutations in coding for Thus, we examined possible miRNA misregulation...
Abstract Glioblastoma is the most frequent and malignant brain tumor, characterized by an elevated capacity for cellular proliferation invasion. Recently, it was demonstrated that podoplanin membrane sialo‐glycoprotein encoded PDPN gene over‐expressed related to invasion in astrocytic tumors; however mechanisms of regulation are still unknown. MicroRNAs noncoding RNAs regulate expression several biological processes diseases, including cancer. Nevertheless, their roles invasion,...
<h3>Objective</h3> MicroRNA (miRNA) expression profile can be used as prognostic marker for human cancers. We aim to explore the significance of miRNAs in colorectal cancer (CRC) metastasis. <h3>Design</h3> performed miRNA microarrays using primary CRC tissues from patients with and without metastasis, validated selected candidates 85 samples by quantitative real-time PCR (qRT-PCR). tested metastatic activity identified targets prediction algorithms, qRT-PCR, western blot luciferase assays....
The epithelial-mesenchymal transition (EMT) imparts metastatic competence on otherwise non-metastatic cancer cells through decreased inter-cellular adhesions, increased migratory capacity, stem cell properties and anoikis chemotherapy resistance. In this study, we profiled changes in microRNA expression during EMT conjunction with DNA methylation at promoters to discover essential mediators of EMT-imparted stemness properties. MicroRNA-203 (miR-203) is repressed following induced by multiple...
Abstract Inflammation and oxidative stress are thought to play determinant roles in the pathogenesis of amyotrophic lateral sclerosis (ALS). Degenerating motor neurons produce signals that activate microglia release reactive oxygen species (ROS) proinflammatory cytokines, resulting a vicious cycle neurodegeneration. The ALS-causing mutant protein Cu+/Zn+ superoxide dismutase SOD1-G93A directly enhances activity main ROS-producing enzyme microglia, NADPH oxidase 2 (NOX2), well-known player...
A common feature of non-coding repeat expansion disorders is the accumulation RNA repeats as foci in nucleus and/or cytoplasm affected cells. These can be toxic by sequestering RNA-binding proteins, thus affecting various steps post-transcriptional gene regulation. However, precise step that C9orf72 GGGGCC (G4C2) expansion, major genetic cause Amyotrophic Lateral Sclerosis, still poorly defined. In this work, we set out to characterise these mechanisms identifying binding proteins....