A5049940781- Wound Healing and Treatments
- Skin and Cellular Biology Research
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Lymphatic System and Diseases
- Mesenchymal stem cell research
- Autoimmune Bullous Skin Diseases
- Dermatological and Skeletal Disorders
- Dermatology and Skin Diseases
- Dermatologic Treatments and Research
- Periodontal Regeneration and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- melanin and skin pigmentation
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Pressure Ulcer Prevention and Management
- Wnt/β-catenin signaling in development and cancer
- Diabetic Foot Ulcer Assessment and Management
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Testicular diseases and treatments
- Thyroid Cancer Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Sexual Differentiation and Disorders
- Cutaneous Melanoma Detection and Management
Istituto Dermopatico dell'Immacolata
2009-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2024
Institute of Cell Biology and Neurobiology
2000-2019
Laboratory of Molecular Genetics
2005
Institute of Molecular and Cell Biology
2003
The Francis Crick Institute
1997-1998
National Institute for Health Research
1998
University of Rome Tor Vergata
1993
Sapienza University of Rome
1989
Th subsets are defined according to their production of lineage-indicating cytokines and functions. In this study, we have identified a subset human cells that infiltrates the epidermis in individuals with inflammatory skin disorders is characterized by secretion IL-22 TNF-alpha, but not IFN-gamma, IL-4, or IL-17. analogy Th17 subset, cytokine profile been named Th22 subset. clones derived from patients psoriasis were stable culture exhibited transcriptome clearly separate those Th1, Th2,...
Abstract Background Reactive oxygen species (ROS) are increased in diabetic conditions and play a causal role foot ulcers (DFU). We previously showed that ROS up-regulate miR-200c expression, turns causes apoptosis, senescence, upregulation nitric oxide decrease, leading to endothelial disfunction. Methods The aim of this study is dissect DFU explore the potential anti-miR-200c antioxidant catalase (CAT) promoting wound healing (WH). inhibition CAT treatment were performed either...
An RNA-binding motif (RBM) gene family has been identified on the human Y chromosome that maps to same deletion interval as 'azoospermia factor' (AZF). We have homologous (Rbm) mouse with a view investigating proposal this plays role in spermatogenesis. At least 25 and probably >50 copies of Rbm are present short arm located between Sry centromere. As human, spermatogenesis is indicated by germ cell-specific pattern expression testis, but there distinct differences two species. Mice carrying...
Placenta growth factor (PlGF) is a member of the vascular endothelial (VEGF) family, comprising at least five cytokines specifically involved in regulation and/or lymphatic endothelium differentiation. Several lines evidence indicate role for PlGF monocyte chemotaxis and potentiating activity VEGF, but exact function this cytokine not fully understood. To define biological vivo, we have produced transgenic mouse model overexpressing skin by using keratin 14 promoter cassette. Our data that...
Abstract Adipose tissue–derived stem cells ( ASC s) are gaining increasing consideration in tissue repair therapeutic application. Recent evidence indicates that s enhance skin animal models of impaired wound healing. To assess the activity autologous vs. allogeneic treatment diabetic ulcers, we functionally characterized and investigated their potential to promote healing with respect nondiabetic ones. from streptozotocin‐induced type 1 mice were analyzed either freshly isolated as stromal...
Lymphatic vessel growth, or lymphangiogenesis, is regulated by vascular endothelial growth factor-C (VEGF-C) and -D via VEGF receptor 3 (VEGFR-3). Recent studies suggest that VEGF, which does not bind to VEGFR-3, can also induce lymphangiogenesis through unknown mechanisms. To dissect the pathway triggers VEGFR-3–independent we used both transgenic adenoviral overexpression of placenta factor (PlGF) VEGF-E, are specific activators VEGFR-1 -2, respectively. Unlike PlGF, VEGF-E induced...
We investigated the expression of microRNAs (miRNAs) associated with replicative senescence in human primary keratinocytes. A cohort miRNAs up-regulated was identified by genome-wide miRNA profiling, and their change validated proliferative versus senescent cells. Among these, (miR)-138, -181a, -181b, -130b increased serial passages. miR-138, but not miR-130b, overexpression proliferating cells sufficient per se to induce senescence, as evaluated inhibition BrdU incorporation quantification...
Recessive dystrophic epidermolysis bullosa (RDEB) is a genodermatosis characterized by fragile skin forming blisters that heal invariably with scars. It due to mutations in the COL7A1 gene encoding type VII collagen, major component of anchoring fibrils connecting cutaneous basement membrane dermis. Identical often result inter- and intra-familial disease variability, suggesting additional modifiers contribute RDEB course. Here, we studied monozygotic twin pair presenting markedly different...
Abstract Integrins are a family of cell surface receptors that mediate cell-cell and cell-matrix interactions in variety different cellular systems. Here we show unfertilized mouse oocytes express β1 class integrins both at mRNA protein levels. Using the reverse transcription polymerase chain reaction oligonucleotide primers based on DNA sequence integrins, RNA transcripts for β1, α5 α6 subunits were detected oocytes. The expression mRNAs is paralleled by corresponding proteins, fact, theα...
Keratinocyte proliferation and migration are crucial steps for the rapid closure of epidermis during wound healing, but molecular mechanisms involved in this cellular response remain to be completely elucidated. Here, by situ hybridization we characterize expression pattern miR-203 after induction mouse epidermis, showing that its is downregulated highly proliferating keratinocytes 'migrating tongue', whereas it strongly expressed differentiating cells skin outside wound. Furthermore,...
Abstract BACKGROUND Melanoma metastasizes by different mechanisms comprising direct invasion of the surrounding tissue and spreading via lymphatic or vascular system. Despite their clinical relevance, molecular that guide route localization metastases in tissues are not well known. Recent studies tumor types have shown endothelial growth factor‐C (VEGF‐C), which displays a high specificity for endothelium, is involved tumor‐induced lymphangiogenesis metastatic spread. The authors studied...
Journal Article Granulocyte/macrophage colony‐stimulating factor treatment of human chronic ulcers promotes angiogenesis associated with de novo vascular endothelial growth transcription in the ulcer bed Get access F. Cianfarani, Cianfarani Laboratory Molecular and Cell Biology, VIII Dermatology Division Search for other works by this author on: Oxford Academic Google Scholar R. Tommasi, Tommasi C.M. Failla, Failla M.T. Viviano, Viviano G. Annessi, Annessi Histopathology Department, Istituto...
Summary. When mouse spermatozoa were briefly exposed in culture to radioactively labelled DNA (pSV2CAT plasmid), radioactivity could be detected by high-resolution autoradiography on the surface and within nucleus of spermatozoa. Spermatozoa from other mammalian species (boar, bull, man) also bind foreign DNA. With exclusion human spermatozoa, which most experiments showed very low labelling values, percentages (evaluated light microscope autoradiography) ranged between 39 78%. In all four...