A5017425688- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Viral Infectious Diseases and Gene Expression in Insects
- Genetically Modified Organisms Research
- Lepidoptera: Biology and Taxonomy
- Insect Pheromone Research and Control
- NF-κB Signaling Pathways
- Forest Insect Ecology and Management
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Insect and Pesticide Research
- interferon and immune responses
- RNA and protein synthesis mechanisms
Bayer (United States)
2019-2022
United Soybean Board
2022
Purdue University West Lafayette
2006
Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind insect receptors compared proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant bioassays, disabled insecticidal protein (DIP) cell-based assays using cells expressing individual demonstrate that receptor utilizations of the newly Cry1B.868...
Two new chimeric Bacillus thuringiensis (Bt) proteins, Cry1A.2 and Cry1B.2, were constructed using specific domains, which provide insecticidal activity against key lepidopteran soybean pests while minimizing receptor overlaps between themselves, current, soon to be commercialized plant incorporated protectants (PIP’s) in soybean. Results from insect diet bioassays demonstrate that the recombinant Cry1B.2 are toxic looper (SBL) Chrysodeixis includens Walker, velvetbean caterpillar (VBC)...
The Western corn rootworm (WCR) Diabrotica virgifera LeConte is one of the most economically important insect pests in North America. Since 2003, transgenic maize expressing WCR-active proteins from Bacillus thuringiensis ( Bt ) have been widely adopted as main approach to controlling WCR U.S. However, emergence field resistance current commercial products has documented recent years, highlighting need develop additional tools for this devasting pest. Here we report discovery Vpb4Da2...
This study describes three closely related proteins, cloned from Brevibacillus laterosporus strains, that are lethal upon feeding to Diabrotica virgifera LeConte, the western corn rootworm (WCR). Mpp75Aa1, Mpp75Aa2 and Mpp75Aa3 were toxic WCR larvae when fed purified protein. Transgenic plants expressing each mMpp75Aa protein protected damage showed significant reduction in adult emergence infested by both susceptible Cry3Bb1 Cry34Ab1/Cry35Ab1-resistant WCR. These results demonstrate...
The western corn rootworm (WCR), Diabrotica virgifera LeConte, is a major pest of significant economic importance in the United States. continuous need to control this maize and development field-evolved resistance toward all existing transgenic ( Zea mays L.) expressing Bacillus thuringiensis Bt ) insecticidal proteins against WCR has prompted new insect-protected crops distinct structural classes proteins. In current study, we describe crystal structure functional characterization...
The western corn rootworm (WCR), Diabrotica virgifera LeConte, is a major maize pest in the United States causing significant economic loss. emergence of field-evolved resistant WCR to Bacillus thuringiensis (Bt) traits has prompted need discover and deploy new insecticidal proteins transgenic maize. In current study we determined crystal structure mode action (MOA) Vpb4Da2 protein (formerly known as Vip4Da2) from Bt, first identified Vpb4 with commercial level control against WCR. exhibits...
The western corn rootworm (WCR),
The recently discovered insecticidal protein Mpp75Aa1.1 from Brevibacillus laterosporus is a member of the ETX_MTX family beta-pore forming proteins (β-PFPs) expressed in genetically modified (GM) maize to control western corn rootworm (WCR; Diabrotica virgifera LeConte). In this manuscript, bioinformatic analysis establishes that although shares varying degrees similarity members ETX_MTX2 family, it unlikely have any allergenic, toxic, or otherwise adverse biological effects. safety further...
ABSTRACT The recently discovered insecticidal protein Mpp75Aa1.1 from Brevibacillus laterosporus is one member of the ETX_MTX family beta-pore forming proteins (β-PFPs) expressed in genetically modified (GM) maize to control western corn rootworm (WCR; Diabrotica virgifera LeConte). In this paper, bioinformatic analysis establishes that although shares varying degrees similarity members ETX_MTX2 family, it unlikely contribute any allergenic, toxic, or otherwise adverse biological effects....