The integration of nanoparticles (NPs) into agriculture is altering traditional methods, enhancing productivity and sustainability. This study explores the application iron oxide (FeONPs) to mitigate salt-alkaline stress in cherry tomatoes. We investigated FeONPs at three concentrations (FeONP25, FeONP50, FeONP100 mg/kg soil) pot experiments under non-stress (NS) (SAS) conditions. SAS conditions decreased biomass nutrients untreated plants, a trend reversed by FeONPs. treatments...

Bell pepper (Capsicum annuum L.) is a commercially important and nutritionally rich vegetable crop in the Solanaceae family. Alkaline stress (AS) can disrupt growth, metabolism, and, particularly, nutritional quality. This study aims to evaluate role of iron oxide nanoparticles (FeNP) mitigating AS enhancing plant growth metabolic functions by conducting experiments under controlled greenhouse conditions with four main treatments: (irrigating plants alkaline salts mixture solution); FeNP...

Abstract Capsaicinoids are a group of chemicals that give chili peppers their characteristic pungency, with capsaicin being significant contributor. Accurately measuring capsaicinoid content is crucial for assessing the quality chili-based food products. Herein, spicy level products was measured using an electrochemical detection tool, Android-based FoodSense G4 (FsG4). In this study, robust evaluation examined to investigate accuracy and precision commercial sensor. The oil- water-based...

Salinity is one of the abiotic stresses that inhibits plant growth and development. One mechanisms by which plants tolerance to salinity through synthesizing salicylic acid (SA). This research was aimed evaluate impact SA on growth, photosynthesis pigments, proline accumulation endogenous hormone levels black rice ‘Sembada Hitam' subjected saline conditions. Black seeds were germinated in a plastic tray containing media seedlings 3 weeks old transplanted into chamber with similar media....

Gelatin is a derivative that can be obtained from the bones of animals such as bovine, goat, and porcine. Ensuring halal status gelatin crucial due to combination derived bovines goats mixed with porcine in order enhance stability its physico-chemical properties for application food cosmetic applications. This study aims authenticate by utilizing metabolomics analysis approach using UHPLC-HRMS chemometrics. The study's findings showed PCA clearly distinguished metabolite profiles bone...

Abstract The sugar yield lost caused by borer is about 10% or equal to 1000 kg of sugar/ha. There no resistance trait stem shoot available in sugarcane germplasm. Genetic engineering expressing the Cry1Ab-Cry1Ac gene fusion efforts develop resistant variety borer. A synthetic which consists Rubisco promoter, chloroplast specific transit peptide (CTP) and was designed assembled for a total size 4019bp. It inserted into pU3775CE plasmid cloning, then pCAMBIA5300_Ubi-tNOS vector at HindIII KpnI...

Abstract WHO recommends artemisinin-based combination therapy for curing malaria which is still a health problem in Indonesia. Artemisia annua L. the primary source artemisinin comes from subtropical China and India. Some introduction materials are grown Indonesia, but they produce low concentration of artemisinin. Artemisinin synthesis uses isoprenoid pathway, farnesyl diphospate (FDP) serves as main precursor. FDP precursor sterol well. The gene controls biosynthesis Squalene synthase (...

<p>Evaluation of resistance double haploid population crosses between IR64 and Oryza rufipogon against Bacterial Leaf Blight (BLB) at seedling stage was conducted during dry season 2005/2006 in the screen house, Rice Centre Research Sukamandi. Inoculum prepared by isolating BLB infected leaf laboratory using Wakimoto’s media. Seeds were germinated petri dish for 48 hours, then sown plastic boxes size 40 cm x 30 cm, each family planted 10 long row. TN1, IRBB, Code, Angke,...

<p>of drought in rice. HVA1 is one of the Late Embryogenesis<br />Abundant (LEA) protein group that plays a role on cell<br />protection during stresses. A study was done with an<br />objective to construct plasmid vector expressing and<br />to transform it into Indonesian elite rice varieties. Materials<br />used were pBY520 (source HVA1;<br />intermediate pRP9; pAY560326<br />(backbone); restriction enzymes BamHI, HindIII, XhoI, />SpeI; T4...

Abstract Cassava ( Manihot esculenta Crantz) is an important staple crop in tropic countries. A reproducible somatic embryogenic callus production will be useful the biotechnology breeding program for plants including cassava. Menti and Adira-4 are two types of high yield cassava genetic resources Indonesia. Immature leaf lobes from vitro were used as explants on MS basal medium with five level concentrations Picloram: 0 mg L −1 , 3 5 10 12 . Initial seen 2 weeks after treatment. Explants...

Foot rot disease caused by Phytophthora capsici is a major problem in pepper cultivation Indonesia and causes rapid plant death. Assembling resistant cultivars through hybridization one strategy for combating foot disease. This study aims to evaluate the hybrid's response vitro inoculation with P. isolates. Ten parents, including wild relative resistance control (Piper colubrinum hirsutum) 33 hybrids of intra-and interspecific crosses were inoculated four isolates (R11, B37, LP41, K10, K2)...

Abstract Wolfiporia cocos, a versatile fungus acclaimed for its nutritional and therapeutic benefits in Traditional Chinese Medicine, holds immense potential pharmaceutical industrial applications. In this study, we aimed to optimize liquid fermentation techniques culture medium composition maximize mycelial biomass (MB) yield, pachymic acid (PA) concentration, overall PA production. Additionally, investigated the molecular basis of our findings by quantifying expression levels genes...

Sugarcane (Saccharum officinarum) is a plant with high economic value because it can produce sugar. Shoot borer Scircophaga excerptalis (Lepidoptera: Pyralidae) and stem Chilo sacchariphagus attacks are one of the issues that limit sugarcane productivity. Establishing transgenic most efficient ways to prevent damage. Previously, Cry1Ab Cry1Ac genes were successfully used create plants. This study aimed detect presence transgenes analyze expression level Cry1Ab-Cry1Ac gene fusion in using...

ABSTRAK Phytophthora capsici adalah penyebab penyakit busuk pangkal batang yang paling merugikan pada lada di Indonesia dan sulit dikendalikan karena dapat bertahan lama dalam tanah serta memiliki keragaman agresivitas isolat luas. Pengetahuan mengenai genetik strain-strain P. membantu perancangan strategi efektif pengelolaan patogen. Penelitian ini bertujuan mengevaluasi struktur isolat-isolat asal menggunakan penanda RAPD. dilaksanakan bulan Oktober 2009 sampai April 2010 Laboratorium...

Introducing cry genes into rice genome is reported able to produce plant resistant stemborer. DNA sequence encodes cry1Ac gene has been inserted pGEM4Z, but this construct does not have a selectable marker for selection of transformed cells. The research aims were plasmid vector expressing that transformation and transform it Agrobacterium tumefaciens. Materials used pAY560325 binary vector, pGEM4Z-cry1Ac Escherichia coli strain DH5-α A. tumefaciens LBA4404 competent methods consisted...

An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of knock-out, so its insertion in genome expected not linked to any dramatic changes plant phenotypes. Gene leading lossof- function (LoF) mutation, a dominant approach for rice functional genomic studies. The objective this study was find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA inducing mutant phenotypes TAFET population. Materials used experiment were T1...

An enhancer trap system is an insertional mutagenesis based upon gene expression, instead of knock-out, so its insertion in genome expected not linked to any dramatic changes plant phenotypes. Gene leading lossof- function (LoF) mutation, a dominant approach for rice functional genomic studies. The objective this study was find out whether Transcriptional Activator-Facilitated Enhancer Trap (TAFET) T-DNA inducing mutant phenotypes TAFET population. Materials used experiment were T1...