A5101960174- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Cancer, Lipids, and Metabolism
- Bacteriophages and microbial interactions
- Ferroptosis and cancer prognosis
- Neuroscience of respiration and sleep
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Single-cell and spatial transcriptomics
- High Altitude and Hypoxia
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- Laser Applications in Dentistry and Medicine
- Plant Virus Research Studies
- Gastrointestinal disorders and treatments
- Acupuncture Treatment Research Studies
- Cell death mechanisms and regulation
- Fungal and yeast genetics research
- Sirtuins and Resveratrol in Medicine
- Adipose Tissue and Metabolism
- Transgenic Plants and Applications
- Connective tissue disorders research
Chengdu University of Traditional Chinese Medicine
2024
Wuhan University
2024
Frontier Science Foundation
2024
Yunnan University
2024
Center for Life Sciences
2024
Wuhan Institute of Virology
2024
State Key Laboratory of Virology
2024
North Sichuan Medical University
2024
Southwest University
2024
Luye Pharma (China)
2024
Adaptive changes in respiratory and cardiovascular responses at high altitude (HA) have been well clarified. However, the central mechanisms underlying HA acclimatization remain unclear. Using voxel-based morphometry (VBM) diffusion tensor imaging (DTI) with fractional anisotropy (FA) calculation, we investigated 28 Han immigrant residents (17–22 yr) born raised of 2616–4200 m Qinghai-Tibetan Plateau for least 17 years who currently attended college sea-level (SL). Their family migrated from...
CRISPR-Cas-based genome editing holds immense promise for advancing plant genomics and crop enhancement. However, the challenge of low activity complicates identification events. In this study, we introduce multiple single transcript unit surrogate reporter (STU-SR) systems to enhance selection genome-edited plants. These use same guide RNAs designed endogenous genes edit genes, establishing a direct link between gene that genes. Various strategies are used restore functional after editing,...
In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating amidst the prevalent rRNA during single-cell RNA-seq. This study introduces novel method, ribosomal RNA-derived cDNA depletion (RiboD), seamlessly integrated into PETRI-seq technique, yielding RiboD-PETRI. innovative approach offers cost-effective, equipment-free, and high-throughput solution for RNA sequencing (scRNA-seq). By efficiently eliminating reads substantially enhancing...
Objective Mucosal immunization was an effective defender against pathogens. Nasal vaccines could activate both systemic and mucosal immunity to trigger protective immune responses. However, due the weak immunogenicity of nasal lack appropriate antigen carriers, very few have been clinically approved for human use, which a major barrier development vaccines. Plant-derived adjuvants are promising candidates vaccine delivery systems their relatively safe immunogenic properties. In particular,...
Promiscuous enzymes are generally considered to be starting points in the evolution of offspring with more specific or even novel catalytic activities, which is molecular basis producing new biological functions. Mhg, a typical α/β fold hydrolase, was previously reported have both γ-lactamase and perhydrolase activities. However, despite having high structural similarity sharing an identical triad extensively studied esterase from Pseudomonas fluorescens, this enzyme did not show any...
The A20 protein is considered to have a potent anti-inflammatory effect, but its mechanism of action in the regulation ferroptosis and inflammation after stroke still unknown. In this study, A20-knockdown BV2 cell line (sh-A20 BV2) was constructed at first, oxygen-glucose deprivation/re-oxygenation (OGD/R) model constructed. Both sh-A20 cells were treated with inducer erastin for 48 h, ferroptosis-related indicators detected by western blot. explored blot immunofluorescence. Under OGD/R...
The 1,3,6,8-tetrabromocarbazole and 3-bromocarbazole have attracted great attention in the ecotoxicology field recently as hazardous environmental contaminants. In this study, quenching mechanism of these two substances binding with human serum albumin (HSA) has been investigated spectroscopic methods. Through fluorescence site experiments steady-state UV-Vis spectra, intrinsic HSA quenched by both static process, are activated to II (subdomain IIIA) HSA. addition, it was not only found that...
Although several regulatory pathways have been reported for Aspergillus flavus, the regulation of aflatoxin production and mycelial growth under different temperatures remains unclear. In this study, A. flavus differentially expressed genes (DEGs) were analyzed three temperatures, by strand-specific RNA-Seq. Results show that a total 2,428 1,474 DEGs identified in fungal mycelia cultured at 20°C 37°C, respectively, as compared with control (28°C). Approximately ~ 79% 37°C samples...
Our previous study showed that the acupuncture stimulation on acupoint (ST‐36) could activate brain‐derived neurotropic factor (BDNF) signaling pathways in telomerase‐deficient mice. Recently, we set out to investigate whether manual (MA) or electroacupuncture (EA) displays a therapeutic advantage age‐related deterioration of learning and memory. Both mice (Terc −/− group, n = 24) wild‐type (WT were randomly assigned 3 subgroups (CON, controls with no treatment; MA, receiving acupuncture;...
Biofilms are complex bacterial communities characterized by a high persister prevalence, which contributes to chronic and relapsing infections. Historically, formation in biofilms has been linked constraints imposed their dense structures. However, we observed an elevated frequency accompanying the stage of cell adhesion, marking onset biofilm development. Subsequent mechanistic studies uncovered comparable type toxin-antitoxin (TA) module (TA-like system) triggered is responsible for this...
Biofilms are complex bacterial communities characterized by a high persister prevalence, which contributes to chronic and relapsing infections. Historically, formation in biofilms has been linked constraints imposed their dense structures. However, we observed an elevated frequency accompanying the stage of cell adhesion, marking onset biofilm development. Subsequent mechanistic studies uncovered comparable type toxin-antitoxin (TA) module (TA-like system) triggered is responsible for this...
Given the high incidence of esophageal cancer in China, an increasing number patients there are undergoing endoscopic mucosal dissection (ESD). Although 5-year survival rate after ESD can exceed 95%, stricture, most common and serious postoperative complication, affects long-term prognosis quality life. Autologous grafts have proven to be successful preventing stricture for early cancer.
Biofilms are complex bacterial communities characterized by a high persister prevalence, which contributes to chronic and relapsing infections. Historically, biofilm formation has been linked constraints imposed their dense structures. However, we observed an elevated frequency accompanying the stage of cell adhesion, marking onset development. Subsequent mechanistic studies uncovered distinctive type toxin-antitoxin (TA) module triggered is responsible for this elevation. In module, toxin...
Biofilms are complex bacterial communities characterized by a high persister prevalence, which contributes to chronic and relapsing infections. Historically, formation in biofilms has been linked constraints imposed their dense structures. However, we observed an elevated frequency accompanying the stage of cell adhesion, marking onset biofilm development. Subsequent mechanistic studies uncovered comparable type toxin-antitoxin (TA) module (TA-like system) triggered is responsible for this...
In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating amidst the prevalent rRNA during single-cell RNA-seq. This study introduces novel method, Ribosomal RNA-derived cDNA Depletion (RiboD), seamlessly integrated into PETRI-seq technique, yielding RiboD-PETRI. innovative approach offers cost-effective, equipment-free, and high-throughput solution for RNA sequencing (scRNA-seq). By efficiently eliminating reads substantially enhancing...
Kyphoscoliotic Ehlers-Danlos syndrome (kEDS; OMIM225400) is a rare autosomal recessive genetic disease caused by variants in the PLOD1 gene. This research was conducted to verify disease-causing gene Chinese neonatal family with EDS.We recruited Han neonate PLOD1-related kEDS without kyphoscoliosis. Detailed clinical examination and laboratory tests were performed whole exome sequencing (WES) used detect pathogenic genes of proband. In vivo experiments (reverse-transcription PCR,...
The accumulation of amyloid beta (Aβ) peptide faulty isoform has been found to cause Alzheimer’s disease (AD) as it is the major component senile plaques in extracellular matrix brains AD patients. Aβ42 (only two amino acid longer than Aβ40) tends aggregate, forming neurotoxic oligomers and fibrils. A lot studies were focused on Aβ42, but all failed explain why production balance Aβ40 leans towards neurons recent study, utilizing NMR technology, was able illustrate molecular mechanism...