A5070927799- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Epigenetics and DNA Methylation
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- Planarian Biology and Electrostimulation
- Genetics, Aging, and Longevity in Model Organisms
- Parasitic infections in humans and animals
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Acute Lymphoblastic Leukemia research
- Cancer-related gene regulation
- Legal Education and Practice Innovations
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- Oral and Maxillofacial Pathology
- Bone Tumor Diagnosis and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- RNA regulation and disease
- Extracellular vesicles in disease
- Medical Malpractice and Liability Issues
- Reproductive System and Pregnancy
- Tuberous Sclerosis Complex Research
- Oral and gingival health research
The University of Texas Southwestern Medical Center
2013-2025
Southwestern Medical Center
2016-2024
Chiba University
2012-2016
University of Michigan
2014
Foundation for Advanced Studies on International Development
2013-2014
Graduate School USA
2012
Schistosome biology illuminated Schistosomiasis is caused by a parasitic flatworm about which little known. Therefore, options to combat human disease schistosome infection are limited. To aid in our quest develop treatments, two studies undertook molecular investigations of the parasite Schistosoma mansoni . By generating single-cell atlas, Wendt et al. identified developmental trajectory flatworm, including blood-feeding gut required for its survival host. From these data, they found gene...
Polycomb group (PcG) proteins are essential regulators of hematopoietic stem cells. Recent extensive mutation analyses the myeloid malignancies have revealed that inactivating somatic mutations in PcG genes such as EZH2 and ASXL1 occur frequently patients with myelodysplastic disorders including syndromes (MDSs) MDS/myeloproliferative neoplasm (MPN) overlap (MDS/MPN). In our patient cohort, were also found often coincided tet methylcytosine dioxygenase 2 (TET2) mutations. Consistent these...
Schistosomes infect more than 200 million people. These parasitic flatworms rely on a syncytial outer coat called the tegument to survive within vasculature of their host. Although is pivotal for survival, little known about maintenance this tissue during decades schistosomes in bloodstream. Here, we demonstrate that relies stem cells (neoblasts) specify fusogenic progenitors replace tegumental lost turnover. Molecular characterization neoblasts and led discovery two flatworm-specific zinc...
Schistosomes infect more than 200 million of the world's poorest people. These parasites live in vasculature, producing eggs that spur a variety chronic, potentially life-threatening, pathologies exacerbated by long lifespan schistosomes, can thrive host for decades. How schistosomes maintain their longevity this immunologically hostile environment is unknown. Here, we demonstrate somatic stem cells Schistosoma mansoni are biased towards generating population expressing factors associated...
P53 is a widely studied tumor suppressor that plays important roles in cell-cycle regulation, cell death, and DNA damage repair. found throughout metazoans, even invertebrates do not develop malignancies. The prevailing theory for why these possess originally evolved to protect the germline of early metazoans from genotoxic stress such as ultraviolet radiation. This largely based upon functional data only three invertebrates, omitting groups animals including flatworms. Previous studies...
<ns4:p>Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different <ns4:italic>Schistosoma</ns4:italic> species. Schistosomes have a complex life cycle, with freshwater snail as intermediate host. After infection, sporocysts develop inside the host and give rise to human dwelling larvae. We present here detailed step-by-step video instruction in English, French, Spanish Portuguese that shows how these can be manipulated...
Schistosomes are blood dwelling parasitic flatworms that can survive in the circulation of their human hosts for decades. These parasites possess a unique syncytial skin-like surface tissue known as tegument is thought to be uniquely adapted survival by mediating evasion host defenses. Previous studies have shown cell bodies within tegumental syncytium turned over and perpetually replaced new cells derived from pool somatic stem called neoblasts. Thus, neoblast-driven homoeostasis has been...
TIF1β is a transcriptional corepressor that recruits repressive chromatin modifiers to target genes. Its biological function and physiological targets in somatic stem cells remain largely unknown. Here, we show essential for the maintenance of hematopoietic (HSCs). Deletion Tif1b mice induced active cycling apoptosis HSCs promoted egression from bone marrow, leading rapid depletion HSCs. Strikingly, Tif1b-deficient showed strong trend ectopic expression nonhematopoietic Levels...
Parasitic helminths are master manipulators of host immunity. Their strategy is complex and involves the release excreted/secreted products, including extracellular vesicles (EVs). The protein miRNA contents EVs have been characterised for many parasitic but, despite reports suggesting importance EV surface carbohydrate structures (glycans) in interactions with target cells thus subsequent effector functions, little known about parasite glycomics. Using lectin microarrays, we identified...
Abstract Schistosomiasis is an ancient and chronic neglected tropical disease that infects over 240 million people kills 200,000 of the world’s poorest every year 1, 2 . There are no vaccines because there only one drug available, need for new therapeutics great. The causative agents this flatworm parasites dwell inside host’s circulation, often decades, where they feed on blood lay eggs which primarily responsible pathology. As metazoans comprised multiple tissue types, understanding...
Schistosomes are parasitic flatworms responsible for the neglected tropical disease schistosomiasis, causing devastating morbidity and mortality in developing world. The parasites protected by a skin-like tegument, maintenance of this tegument is controlled schistosome ortholog tumor suppressor TP53. To understand mechanistically how p53-1 controls production, we identified cyclin dependent kinase inhibitor homolog (cki) that was co-expressed with p53-1. RNA interference cki resulted...
Schistosomes are blood-dwelling parasitic flatworms that rely on a syncytial surface coat, known as the tegument, for long-term survival and immune evasion in blood of their human hosts. Previous studies have shown cells within tegumental syncytium perpetually turned over renewed by somatic stem called neoblasts. Yet, little is about this renewal process molecular level. Here, we characterized Kruppel-like factor 4 ( klf4 ) using combination bulk single cell RNAseq approaches demonstrate...
Oxamniquine (OXA) is a prodrug activated by sulfotransferase (SULT) that was only active against Schistosoma mansoni. We have reengineered OXA to be effective S. haematobium and japonicum. Three derivatives stand out, CIDD-0066790, CIDD-0072229, CIDD-0149830 as they kill all three major human schistosome species. However, questions remain. Is the mode of action conserved in derivatives? RNA-interference experiments demonstrate knockdown SmSULT, ShSULT, SjSULT results resistance CIDD-0066790....