A5020011096- Metabolism, Diabetes, and Cancer
- Amino Acid Enzymes and Metabolism
- Adipose Tissue and Metabolism
- Biochemical Acid Research Studies
- Microbial metabolism and enzyme function
- Ion channel regulation and function
- Pancreatic function and diabetes
- Birth, Development, and Health
- Metabolism and Genetic Disorders
- Folate and B Vitamins Research
- Gestational Diabetes Research and Management
- Muscle Physiology and Disorders
- Alcoholism and Thiamine Deficiency
- Muscle metabolism and nutrition
- Polyamine Metabolism and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Cellular transport and secretion
- Ion Transport and Channel Regulation
- Parkinson's Disease Mechanisms and Treatments
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Lipid metabolism and disorders
- Porphyrin Metabolism and Disorders
- Growth Hormone and Insulin-like Growth Factors
- Prenatal Substance Exposure Effects
Universitat de Barcelona
2003-2024
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2014-2024
Instituto de Salud Carlos III
2014-2024
Institut de Biomedicina de la Universitat de Barcelona
2024
Institute for Research in Biomedicine
2007-2021
Inserm
2002-2017
Institut des Maladies Métaboliques et Cardiovasculaires
2017
Université Toulouse III - Paul Sabatier
2017
Institució Catalana de Recerca i Estudis Avançats
2004
Universitat Autònoma de Barcelona
2004
Mitofusin-2 (Mfn2) is a mitochondrial membrane protein that participates in fusion mammalian cells and mutations the Mfn2 gene cause Charcot–Marie–Tooth neuropathy type 2A. Here, we show loss-of-function inhibits pyruvate, glucose fatty acid oxidation reduces potential, whereas gain-of-function increases potential. As to mechanisms involved, have found represses nuclear-encoded subunits of OXPHOS complexes I, II, III V, overexpression induced IV V. Obesity-induced deficiency rat skeletal...
We have isolated a cDNA clone by screening rabbit kidney cortex library for expression of sodium-independent transport L-arginine and L-alanine in Xenopus laevis oocytes. Expressed uptake relates to single component dibasic neutral amino acids. This activity resembles the functionally defined system b0,+ carries cystine acids with high affinity. The rBAT (b0,+ acid transporter-related) mRNA is found mainly intestinal mucosa. It encodes predicted 77.8-kDa protein only one putative...
Skeletal muscle differentiation involves myoblast alignment, elongation, and fusion into multinucleate myotubes, together with the induction of regulatory structural muscle-specific genes. Here we show that two phosphatidylinositol 3-kinase inhibitors, LY294002 wortmannin, blocked an essential step in skeletal cell models. Both inhibitors abolished capacity L6E9 myoblasts to form without affecting proliferation, or alignment. Myogenic events like myogenin glucose carrier GLUT4 were also...
The expression of GLUT-1 (erythroid/Hep G2) and GLUT-4 (muscle/fat) glucose transporters was assessed during development in rat heart, skeletal muscle, brown adipose tissue. protein detectable fetal heart by day 21 pregnancy; it increased progressively after birth, attaining levels close to those adults at 15 post natal. In contrast, messenger RNA (mRNA) already present hearts from 17 day-old fetuses. mRNA stayed low early postnatal life tissue only 10 pattern for muscle comparable that...
Mutations in the rBAT gene cause type I cystinuria, a common inherited aminoaciduria of cystine and dibasic amino acids due to their defective renal intestinal reabsorption (Calonge, M. J., Gasparini, P., Chillarón, Chillón, M., Gallucci, Rousaud, F., Zelante, L., Testar, X., Dallapiccola, B., Di Silverio, Barceló, Estivill, Zorzano, A., Nunes, V., Palacín, (1994) Nat. Genet. 6, 420-426; Calonge, Volipini, Bisceglia, De Sanctis, Beccia, E., (1995) Proc. Natl. Acad. Sci. U. S. A. 92,...
Using isolated rat cardiomyocytes we have examined: 1) the effect of insulin on cellular distribution glucose transporter 4 (GLUT4) and GLUT1, 2) total amount these transporters, 3) co-localization GLUT4, secretory carrier membrane proteins (SCAMPs) in intracellular membranes. Insulin induced 5.7- 2.7-fold increases GLUT4 GLUT1 at cell surface, respectively, as determined by nonpermeant photoaffinity label...
The previous characterization of an abundant population non-adrenergic imidazoline-I<sub>2</sub>binding sites in adipocytes and the recent demonstration interplay between these binding amine oxidases led us to analyze oxidase activity membranes from isolated rat adipocytes. Adipocyte had substantial levels semicarbazide-sensitive (SSAO). SSAO immunoreactive protein were maximal plasma membranes, they also detectable intracellular membranes. Vesicle immunoisolation analysis indicated that...
1. GLUT-4 glucose-transporter protein and mRNA levels were assessed in heart, red muscle white muscle, as well brown adipose tissue from 7-day streptozotocin-induced diabetic 48 h-fasted rats. 2. In agreement with previous data, showed a substantial decrease response to both diabetes fasting. Similarly, markedly decreased insulinopenic conditions. 3. Under control conditions, the level of expression content differed substantially skeletal muscle. Thus was maximal had greater compared spite...
A kidney cortex cDNA clone (rBAT) has recently been isolated, which upon in vitro transcription and capping complementary RNA (cRNA) injection into Xenopus laevis oocytes induces a system b0,(+)-like amino acid transport activity. This encodes type II membrane glycoprotein that shows significant homology to another glycoprotein, the heavy chain of human mouse 4F2 surface antigen (4F2hc). Here we demonstrate 4F2hc cRNA results activation cation-preferring appears be identical y(+)-like...
A renal cDNA clone (rBAT) that induces system bo,+-like amino acid transport activity in Xenopus oocytes has recently been isolated (Bertran, J., Werner, A., Moore, M. L., Strange, G., Markovich, D., Biber, Testar, X., Zorzano, Palacin, and Murer, H. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5601-5605). Here we show the isolation of a by screening human kidney cortex library for expression sodium-independent L-[3H]arginine oocytes. The cRNA this oocytes, addition to uptake L-arginine,...
A precise balance between protein degradation and synthesis is essential to preserve skeletal muscle mass. Here, we found that TP53INP2, a homolog of the Drosophila melanogaster DOR regulates autophagy in cellular models, has direct impact on mass vivo. Using different transgenic mouse demonstrated muscle-specific overexpression Tp53inp2 reduced mass, while deletion resulted hypertrophy. TP53INP2 activated basal sustained p62-independent autophagic ubiquitinated proteins. Animals with...
Insulin-like growth factors (IGFs) are potent inducers of skeletal muscle differentiation and phosphatidylinositol (PI) 3-kinase activity is essential for this process. Here we show that IGF-II induces nuclear factor-kappaB (NF-kappaB) nitric-oxide synthase (NOS) activities downstream from PI these events critical myogenesis. Differentiation rat L6E9 myoblasts with transiently induced NF-kappaB DNA binding activity, inducible (iNOS) expression, nitric oxide (NO) production. IGF-II-induced...
Abstract We have recently isolated a renal cDNA clone (rBAT) that induces amino acid transport in oocytes either as component or specific activator of system bo,(+)-like transporter. (Bertran, J., Werner, A., Moore, M. L., Stange, G., Markovich, D., Biber, Testar, X., Zorzano, Palacin, M., and Murer, H. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 5601-5605. In order to obtain additional information on the rBAT protein, we investigated cellular localization its expression during development...
Cystinuria is an autosomal recessive amino-aciduria where three urinary phenotypes have been described (I, II, and III). An amino acid transporter gene, SLC3A1 (formerly rBAT), was found to be responsible for this disorder. To assess whether mutations in are involved different cystinuria phenotypes, linkage with gene its nearest marker (D2S119) analyzed 22 families type I and/or III cystinuria. Linkage heterogeneity proved (alpha = 0.45; P < 0.008). Type I/I showed homogeneous (Zmax > 3.0 at...
GLUT1 and GLUT4 glucose transporter expression is highly regulated in muscle adipose tissue during perinatal life. Here we have investigated the role of thyroid hormones regulation induction repression associated to neonatal development. Perinatal hypothyroidism markedly impaired protein heart. This effect was heart specific, a greater detected brown from hypothyroid rats compared with controls. These changes were not or when induced adult rats. results indicate that life sensitive both...
Phosphatidylinositol 3 (PI 3)-kinases are potently inhibited by two structurally unrelated membrane-permeant reagents: wortmannin and LY294002. By using these inhibitors we first suggested the involvement of a PI 3-kinase activity in muscle cell differentiation. However, several reports have described that compounds not as selective for assumed. Here show LY294002 blocks myogenic pathway elicited insulin-like growth factors (IGFs), confirm specific IGF-induced myogenesis overexpressing L6E9...
The human rBAT protein elicits sodium-independent, high affinity obligatory exchange of cystine, dibasic amino acids, and some neutral acids in Xenopus oocytes (Chillarón, J., Estévez, R., Mora, C., Wagner, C. A., Suessbrich, H., Lang, F., Gelpí, J. L., Testar, X., Busch, A. E., Zorzano, Palacín, M. (1996) Biol. Chem. 271, 17761-17770). Mutations have been found to cause cystinuria (Calonge, Gasparini, P., Chillarón, Chillón, M., Galluci, Rousaud, Zelante, Dallapiccola, B., Di Silverio,...
During lactation, the mammary gland shows high metabolic activity, which is dependent at least in part on availability of glucose. We have studied regulation glucose transporter expression different types cell rat during reproductive cycle. Glucose varied markedly ontogeny. Thus, GLUT1 protein increased progressively pregnancy, reaching highest levels lactation. A peak content, expressed per g issue, was greater than GLUT1-enriched tissues such as brain. In contrast, GLUT4 showed a marked...
4F2hc is an almost ubiquitous transmembrane protein in mammalian cells; upon expression Xenopus laevis oocytes, it induces amino acid transport with characteristics of system y+L. Indirect evidence fostered speculation that function requires the association another endogenous to oocytes and native tissues. We show y+L-like activity by limited factor direct covalent modification external cysteine residue(s) oocyte membrane blocks y+ L/4F2hc activity, based on following. 1) Induction saturates...
It has been shown that the combination of benzylamine or tyramine and low concentrations vanadate markedly stimulates glucose transport in rat adipocytes by a mechanism requires semicarbazide-sensitive amine oxidase (SSAO) activity H(2)O(2) formation. Here we have further analysed insulin-like effects SSAO substrates studied signal-transduction pathway activated adipocytes. We found several (benzylamine, tyramine, methylamine, n-decylamine, histamine, tryptamine beta-phenylethylamine), with...
1. GLUT5 gene expression was studied in small intestine under a variety of conditions characterized by altered intestinal absorption monosaccharides. 2. RNA-blotting studies showed that mRNA abundantly expressed rat and rabbit kidney, but it not detected heart or brown adipose tissue. levels were higher the upper segments (duodenum proximal jejunum) than lower (distal jejunum ileum). 3. The circadian rhythm. A 12-fold increase at end light cycle beginning dark when compared with early...