A5101330424- Telomeres, Telomerase, and Senescence
- RNA Research and Splicing
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Graphene and Nanomaterials Applications
- Nanocluster Synthesis and Applications
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Carbon and Quantum Dots Applications
- Lung Cancer Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Nuclear Structure and Function
- Plant Genetic and Mutation Studies
- Urban Green Space and Health
- Machine Learning in Bioinformatics
- Synthesis of Tetrazole Derivatives
- Synthesis and Reactions of Organic Compounds
- Cancer Research and Treatments
- Plant Reproductive Biology
Imperial College London
2020-2024
Sun Yat-sen University
2021-2024
Sun Yat-sen University Cancer Center
2021-2024
Huashan Hospital
2024
Fudan University
2023-2024
State Key Laboratory of Genetic Engineering
2024
MRC London Institute of Medical Sciences
2021-2024
Shandong University of Finance and Economics
2023
Huazhong Agricultural University
2023
Yunnan University of Traditional Chinese Medicine
2023
Cellular imaging after transplantation may provide important information to determine the efficacy of stem cell therapy. We have reported that graphene quantum dots (GQDs) are a type robust biological labeling agent for cells demonstrate little cytotoxicity. In this study, we examined interactions GQDs on human neural (hNSCs) with aim investigate uptake and biocompatibility GQDs. mechanism GQD by hNSCs investigated effects proliferation, metabolic activity, differentiation potential hNSCs....
Sir2, a NAD-dependent deacetylase, modulates lifespan in yeasts, worms and flies. The SIRT1, mammalian homologue of regulates signaling for favoring survival stress. But whether SIRT1 has the function to influence cell viability senescence under non-stressed conditions human diploid fibroblasts is far from unknown. Our data showed that enforced expression promoted proliferation antagonized cellular with characteristic features delayed Senescence-Associated beta-galactosidase (SA-beta-gal)...
Dysregulation of long noncoding RNAs (lncRNAs) contributes to tumorigenesis by modulating specific cancer-related pathways, but the roles N6-methyladenosine (m6A)-enriched lncRNAs and underlying mechanisms remain elusive in nasopharyngeal carcinoma (NPC). Here, we reanalyzed previous genome-wide analysis lncRNA profiles 18 pairs NPC normal tissues as well ten paired samples from with or without post-treatment metastases. We discerned that an oncogenic m6A-enriched lncRNA, LINC00839, which...
Background p16INK4a tumor suppressor protein has been widely proposed to mediate entrance of the cells into senescent stage. Promoter gene contains at least five putative GC boxes, named GC-I V, respectively. Our previous data showed that a potential Sp1 binding site, within promoter region from −466 −451, acts as positive transcription regulatory element. These results led us examine how and/or Sp3 act on these boxes during aging in cultured human diploid fibroblasts. Methodology/Principal...
Short interfering RNA (siRNA)-based therapy has a high potential for application in cancer gene therapy. However, delivery of siRNA to target cells is limited by many factors such as serum ribonuclease (RNase) degradation, off-target effects, and inadequate cellular uptake. In this study, an enzyme-response PEG/Lipids/calcium phosphate hybrid system was constructed siRNA. The nearly neutral charged siRNA@NP2 resistant serum-induced degradation. Compared with the non-enzyme-response...
4-Hydroxy-5-hydroxymethyl-[1,3]dioxolan-2,6'-spirane-5',6',7',8'-tetrahydro-indolizine-3'-carbaldehyde (HDTIC)-1 and HDTIC-2 are two isomers extracted from Astragalus membranaceus (Fisch) Bunge Var. mongholicus (Bge) Hsiao. Our previous study had demonstrated that they could extend the lifespan of human fetal lung diploid fibroblasts (2BS). To investigate mechanisms HDTIC-induced delay replicative senescence, in this study, we assessed effects these compounds on telomere shortening rate DNA...
Bufalin has significant antitumor effects on various kinds of tumors. However, bufalin's clinical utility is severely limited because its side effects, toxicity, and fast metabolism. We made bufalin-loaded, targeted nanospheres with the aim overcoming limitations. Epidermal growth factor (EGF)-modified encapsulated bufalin showed increased toxicity in colorectal cancer cells, inhibition cell proliferation, induction apoptosis relative to non-EGF-modified nanospheres. The average particle...
Human embryonic stem cells (hESCs) are extremely delicate, and survive poorly under suboptimal culture conditions, severely restricting long-term studies practical applications. Thus, a protective agent that promotes cell survival is urgently needed. In this study, we evaluated the effects of stemazole in single-cell starved hESC cultures. Colony formation was quantified by alkaline phosphatase immunofluorescence staining, while apoptosis assessed flow cytometry TUNEL assay. Expression other...
6003 Background: Patients suffering from locoregionally advanced nasopharyngeal carcinoma (NPC) commonly develop disease recurrence, despite a high rate of complete clinical remission after standard care (concurrent cisplatin-radiotherapy, with or without induction chemotherapy). The benefit additional adjuvant chemotherapy remains unclear. Methods: high-risk NPC (stage III to IVA, excluding T3-4N0 and T3N1), no locoregional distant metastasis definitive chemoradiotherapy, were eligible....
Abstract Background Pulmonary nodule growth rate assessment is critical in the management of subsolid pulmonary nodules (SSNs) during clinical follow-up. The present study aimed to develop a model predict SSNs. Methods A total 273 growing SSNs with information and 857 computed tomography (CT) scans were retrospectively analyzed. images randomly divided into training validation sets. All categorized fast-growth (volume doubling time (VDT) ≤ 400 days) slow-growth (VDT > groups. Models for...
Distributed computing involves systems that operate across networks transparently, using the resources of multiple machines. The Open Software Foundation's Computing Environment (DCE) has evolved to address need for a vendor-neutral platform which distributed applications can be developed, and upon they run. Central design philosophy DCE is its reliance on remote procedure call (RPC) facilitate communication among entities in environment. Since it profoundly affects performance both...
The study focuses on examining how the total factor productivity (TFP) of China’s agriculture-related enterprises is affected by reform “County Administrated Province” (CAP). Using panel data from 1998 to 2013, with a sample size 292, 423 enterprises, investigates influence CAP TFP. findings reveal significant dampening effect TFP enterprises. These results are further supported series robustness tests including placebo test, multidimensional fixed effects and clustering cities or counties....
Fritillaria crassicaulis S. C. Chen is a precious traditional Chinese medicine, but the number of populations has declined rapidly due to overexploitation. An artificial rapid propagation system was established screen suitable plant regeneration method and explore efficient method, useful for technology or further research development F. crassicaulis. This study selected scale as experimental material, set Murashige Skoog (MS) medium basic medium, optimized types proportions growth regulator...
Predicting genetic mutations from whole slide images is indispensable for cancer diagnosis. However, existing work training multiple binary classification models faces two challenges: (a) Training classifiers inefficient and would inevitably lead to a class imbalance problem. (b) The biological relationships among genes are overlooked, which limits the prediction performance. To tackle these challenges, we innovatively design Biological-knowledge enhanced PathGenomic multi-label Transformer...