A5023671990- Atherosclerosis and Cardiovascular Diseases
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Cardiovascular Disease and Adiposity
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Congenital heart defects research
- Nuclear Receptors and Signaling
- Estrogen and related hormone effects
- Protease and Inhibitor Mechanisms
- Coronary Interventions and Diagnostics
- Modeling and Simulation Systems
- Caveolin-1 and cellular processes
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Cardiomyopathy and Myosin Studies
- Immune cells in cancer
- Bioinformatics and Genomic Networks
- Vitamin C and Antioxidants Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cell Adhesion Molecules Research
- Genetic Associations and Epidemiology
- Biomedical Ethics and Regulation
University Medical Center Utrecht
2021-2024
Utrecht University
2023
University of Zurich
2018-2020
Swiss Institute for Regenerative Medicine
2018
Hospital Universitario Fundación Jiménez Díaz
2018
Centre for Biomedical Network Research on Rare Diseases
2018
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2018
Pharmo Institute
2018
Instituto de Investigación Sanitaria Aragón
2018
Universidad Carlos III de Madrid
2018
Genetic cardiomyopathies are characterized by changes in the function and structure of myocardium. The development a novel vitro model could help to better emulate healthy diseased human heart conditions may improve understanding disease mechanisms. In this study, for first time, we demonstrated generation cardiac organoids using triculture approach induced pluripotent stem-cell-derived cardiomyocytes (hiPS-CMs)-from subjects cardiomyopathy patients-human microvascular endothelial cells...
Abstract Background After myocardial infarction, the lost myocardium is replaced by fibrotic tissue, eventually progressively leading to dysfunction. Direct reprogramming of fibroblasts into cardiomyocytes via forced overexpression cardiac transcription factors Gata4, Mef2c, and Tbx5 (GMT) offers a promising strategy for repair. The limited efficiency this approach, however, remains significant challenge. Methods We screened seven capable improving direct both mice human evaluating small...
Women presenting with coronary artery disease more often present fibrous atherosclerotic plaques, which are currently understudied. Phenotypically modulated smooth muscle cells (SMCs) contribute to atherosclerosis in women. How these phenotypically SMCs shape female versus male plaques is unknown.
Drug-induced kidney injury in medicinal compound development accounts for over 20% of clinical trial failures and involves damage to different nephron segments, mostly the proximal tubule. Yet, currently applied cell models fail reliably predict nephrotoxicity; neither are such easy establish. Here, we developed a novel three-dimensional (3D) nephrotoxicity platform on basis decellularized rat scaffolds (DS) recellularized with conditionally immortalized human renal tubule epithelial cells...
Sex differences in atherosclerosis have been described with female plaques being mostly perceived as stable and fibrous. Sex-specific mechanisms such mosaic loss of the Y chromosome men linked to cardiovascular health. In women, X-linked X inactivation (XCI) skewing is common several tissues. Yet, information on role XCI atherosclerotic lacking. Here, we investigated presence advanced lesions its association risk factors, histological plaque data, clinical data. was quantified 154 55 blood...
Background Plaque myofibroblasts are critical players in the initiation and advancement of atherosclerotic disease. They involved production extracellular matrix, formation fibrous cap, underlying lipidic core via modulation processes response to different environmental cues. Despite clear phenotypic differences between myofibroblast cells healthy vascular smooth muscle cells, still widely used as a cellular model research. Methods Results Here, we present conditioned outgrowth method...
Abstract Women presenting with coronary artery disease (CAD) more often present fibrous atherosclerotic plaques, which are currently understudied. Phenotypically modulated smooth muscle cells (SMCs) contribute to atherosclerosis in women. How these phenotypically SMCs shape female versus male plaques is unknown. Here, we show sex-stratified gene regulatory networks (GRNs) from human carotid tissue. Prioritization of identified two main SMC GRNs late-stage atherosclerosis. Single-cell...
Abstract Plaque smooth muscle cells are critical players in the initiation and advancement of atherosclerotic disease. They produce extracellular matrix (ECM) components, which play a role lesion progression stabilization. Despite clear phenotypic differences between plaque vascular (VSMCs), VSMCs still widely used as model system research. Here we present conditioned outgrowth method to isolate cells. We obtained from 27 donors (24 carotid 3 femoral endarterectomies). show that these keep...
Abstract Background and aim: Sex differences in atherosclerosis have been described with female plaques being mostly perceived as stable fibrous. Sex-specific mechanisms such mosaic loss of the Y chromosome men linked to cardiovascular health. In women, X-linked X inactivation (XCI) skewing is common several tissues. Yet, information on role XCI atherosclerotic lacking. Here, we investigated presence advanced lesions its association risk factors, histological plaque data, clinical data....