A5013161299- Atherosclerosis and Cardiovascular Diseases
- Synthesis and biological activity
- Adipokines, Inflammation, and Metabolic Diseases
- Single-cell and spatial transcriptomics
- Telomeres, Telomerase, and Senescence
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Mechanisms and Therapy
- Sirtuins and Resveratrol in Medicine
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Cardiovascular Disease and Adiposity
- Multicomponent Synthesis of Heterocycles
- Metabolism, Diabetes, and Cancer
- Antioxidant Activity and Oxidative Stress
- Bioactive Compounds and Antitumor Agents
- Cancer, Lipids, and Metabolism
- Genetic Associations and Epidemiology
- Synthesis and Biological Evaluation
- Synthesis of heterocyclic compounds
- Synthesis and Characterization of Heterocyclic Compounds
- Garlic and Onion Studies
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- Kruppel-like factors research
- Nephrotoxicity and Medicinal Plants
University of Virginia
2020-2024
German Centre for Cardiovascular Research
2024
Indian Institute of Chemical Technology
2012-2022
Academy of Scientific and Innovative Research
2015-2022
Council of Scientific and Industrial Research
2014
Monocyte-to-macrophage differentiation is a critical event that accentuates atherosclerosis by promoting an inflammatory environment within the vessel wall. In this study, we investigated molecular mechanisms responsible for monocyte-to-macrophage and, subsequently, effect of metformin in regressing angiotensin II (Ang-II)-mediated atheromatous plaque formation ApoE−/− mice. AMPK activity was dose and time dependently downregulated during phorbol myristate acetate (PMA)-induced...
Rupture and erosion of advanced atherosclerotic lesions with a resultant myocardial infarction or stroke are the leading worldwide cause death. However, we have limited understanding identity, origin, function many cells that make up late-stage lesions, as well mechanisms by which they control plaque stability.
Thromboembolic events, including myocardial infarction (MI) or stroke, caused by the rupture erosion of unstable atherosclerotic plaques are leading cause death worldwide. Although most mouse models atherosclerosis develop lesions in aorta and carotid arteries, they do not advanced coronary artery lesions. Moreover, undergo spontaneous plaque with MI stroke so at such a low frequency that viable experimental to study late-stage thrombotic events identify novel therapeutic approaches for...
The use of senolytic agents to remove senescent cells from atherosclerotic lesions is controversial. A common limitation previous studies the failure rigorously define effects agent ABT-263 (Navitoclax) on smooth muscle (SMC) despite claiming that these are major source cells. Moreover, there no effect endothelial (EC), which - along with SMC comprise 90% α-smooth actin+ (α-SMA+) myofibroblast-like in protective fibrous cap. Here we tested hypothesis treatment advanced mice will reduce...
Although the anti-cancer effects of curcumin has been shown in various cancer cell types, vitro, pre-clinical and clinical studies showed only a limited efficacy, even at high doses. This is presumably due to low bioavailability both plasma tissues, particularly poor intracellular accumulation. A variety methods have developed achieve selective targeting drugs cells mitochondrion. We used novel approach by conjugation lipophilic triphenylphosphonium (TPP) cation facilitate delivery...
Abstract Mitochondria-targeted compounds are emerging as a new class of drugs that can potentially alter the pathophysiology those diseases where mitochondrial dysfunction plays critical role. We have synthesized novel mitochondria-targeted esculetin (Mito-Esc) with an aim to investigate its effect during oxidative stress-induced endothelial cell death and angiotensin (Ang)-II-induced atherosclerosis in ApoE −/− mice. Mito-Esc but not natural treatment significantly inhibited H2O2-...
Recent studies have highlighted the involvement of metadherin (MTDH), an oncogenic protein, in promoting cancer progression, metastasis and chemoresistance many cancers including mammary carcinomas. However, molecular regulation MTDH is still not completely understood. In this study we document that AMP activated protein kinase (AMPK) activation-induced anti-proliferative effects are, part, mediated by inhibiting expression MDA-MB-231 BT-549 triple negative breast (TNBC) cells....
Women presenting with coronary artery disease more often present fibrous atherosclerotic plaques, which are currently understudied. Phenotypically modulated smooth muscle cells (SMCs) contribute to atherosclerosis in women. How these phenotypically SMCs shape female versus male plaques is unknown.
Accumulating evidence from in vitro, vivo, clinical and epidemiological studies shows promising results for the use of statins against many cancers including breast carcinoma. However, molecular mechanisms responsible anti-proliferative anti-invasive properties still remain elusive. In this study, we investigated involvement nitric oxide, iron homeostasis antioxidant defence mediating hydrophobic MDA-MB-231, MDA-MB-453 BT-549 metastatic triple negative cancer cells. Fluvastatin simvastatin...
Smooth muscle cells (SMCs), which make up the medial layer of arteries, are key cell types involved in cardiovascular disease, leading cause mortality and morbidity worldwide. In response to microenvironment alterations, SMCs dedifferentiate from a contractile synthetic phenotype characterized by an increased proliferation, migration, production ECM (extracellular matrix) components, decreased expression SMC-specific markers. These phenotypic changes result vascular remodeling contribute...
Abstract A library of imidazopyridine–oxindole conjugates was synthesised and investigated for anticancer activity against various human cancer cell lines. Some the tested compounds, such as 10 a , e f k exhibited promising antiproliferative with GI 50 values ranging from 0.17 to 9.31 μ M . Flow cytometric analysis showed that MCF‐7 cells treated by these compounds arrested in G 2 /M phase cycle concentration‐dependent manner. More particularly, compound displayed remarkable inhibitory...
Objective: Smooth muscle cells and pericytes display remarkable plasticity during injury disease progression. Here, we tested the hypothesis that perivascular give rise to Klf4 -dependent macrophage-like augment adipose tissue (AT) inflammation metabolic dysfunction associated with diet-induced obesity (DIO). Approach Results: Using Myh11-Cre ERT2 eYFP (enhanced yellow fluorescent protein) mice flow cytometry of stromovascular fraction epididymal AT, observed a large smooth lineage traced +...
In this study we explored the microRNAs responsible for regulation of PAI‐1 during LPS‐stimulated inflammation in human aortic endothelial cells and subsequently studied effect a newly synthesized mitochondria‐targeted esculetin (Mito‐Esc) that was shown its anti‐atherosclerotic potential, modulating levels targeted miRs angiotensin‐II‐induced atherosclerosis ApoE −/− mice. accompanied with an upregulation miR‐19b down‐regulation miR‐30c. These effects LPS on were reversed presence both...
We report the first high affinity neutral Bodipy fluorophores for selective imaging of mitochondria with notable sensitivity (∼100 nM) and insignificant cytotoxicity even at very concentration μM), when tested against HeLa cells. Further, these are chemically robust require no special conditions storage.
Abstract Multidrug resistance mediated by ATP‐binding cassette (ABC) transporters remains a major impediment to cancer chemotherapy. In the present study, we documented that doxorubicin (Dox) or cisplatin‐induced prostate (PCa) chemoresistance is predominantly induction of ABCG4 in androgen‐independent PCa cells. Treatment DU‐145 PC‐3 cells with Dox significantly enhanced expression resulted efflux intracellular Dox. However, incubation short hairpin RNA significant accumulation and...
A library of imidazothiadiazole–chalcone conjugates were synthesised and investigated for their cytotoxic activity against various human cancer cell lines. Some the tested compounds like<bold>7a</bold>,<bold>7b</bold>,<bold>11a</bold>and<bold>11b</bold>exhibited promising anticancer activity.
DOT1L, a specific H3K79 methyltransferase, has tumour-promoting role in various cancers, including triple-negative breast cancer (TNBC). However, the molecular mechanism by which deregulated DOT1L promotes progression is unclear. Herein, we show that significantly higher basal level of DOTL1 strongly correlates with MTDH, an oncogene, clinical TNBC patient cohorts and mediates enhancing MTDH-induced angiogenesis. In parallel, severe combined immunodeficiency mice-bearing MDA-MB-231 cells...