A5037936416- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Cardiac Imaging and Diagnostics
- Ultrasound in Clinical Applications
- Coronary Interventions and Diagnostics
- Ultrasound Imaging and Elastography
- Cardiovascular Disease and Adiposity
- Venous Thromboembolism Diagnosis and Management
- Cardiovascular Function and Risk Factors
- Heart Rate Variability and Autonomic Control
- Advanced MRI Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Ultrasound and Cavitation Phenomena
- Atherosclerosis and Cardiovascular Diseases
- Acute Myocardial Infarction Research
- Cardiac Valve Diseases and Treatments
- Platelet Disorders and Treatments
- Thermoregulation and physiological responses
- Hemodynamic Monitoring and Therapy
- Cardiovascular Health and Disease Prevention
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Pregnancy and preeclampsia studies
- Cardiovascular and exercise physiology
- Cardiac Arrhythmias and Treatments
Oregon Health & Science University
2015-2025
University of Virginia Medical Center
1995-2025
University of Virginia
2004-2025
University of Virginia Health System
2000-2023
Oregon National Primate Research Center
2008-2022
University of Nebraska Medical Center
2022
Cardiff University
2022
Park Plaza Hospital
2022
Cardiovascular Institute of the South
2017-2021
Lindner Center of HOPE
2007-2019
Angiogenesis is a critical determinant of tumor growth and metastasis. We hypothesized that contrast-enhanced ultrasound (CEU) with microbubbles targeted to alpha(v)-integrins expressed on the neovascular endothelium could be used image angiogenesis.Malignant gliomas were produced in 14 athymic rats by intracerebral implantation U87MG human glioma cells. On day or 28 after implantation, CEU was performed alpha(v)beta3 surface conjugation echistatin. perfusion imaging nontargeted derive...
Routine methods capable of assessing tissue inflammation noninvasively are currently not available. We hypothesized that retention microbubbles targeted to the endothelial cell adhesion molecule P-selectin would provide a means assess with ultrasound imaging.Phospholipid (MB(p)) were created by conjugating monoclonal antibodies against murine lipid shell. The microvascular behaviors MB(p) and control without antibody (MB) or isotype (MB(iso)) assessed intravital microscopy cremasteric...
Insulin increases glucose disposal into muscle. In addition, in vivo insulin elicits distinct nitric oxide synthase-dependent vascular responses to increase total skeletal muscle blood flow and recruit capillaries (by relaxing resistance terminal arterioles, respectively). the current study, we compared temporal sequence of metabolic a 30-min physiological infusion (3 mU · min−1 kg−1, euglycemic clamp) or saline rat vivo. We used contrast-enhanced ultrasound continuously quantify...
Background— The ability to image vascular inflammatory responses may allow early diagnosis and treatment of atherosclerosis. We hypothesized that molecular imaging cell adhesion molecule-1 (VCAM-1) expression with contrast-enhanced ultrasound (CEU) could be used for this purpose. Methods Results— Attachment VCAM-1–targeted control microbubbles cultured endothelial cells was assessed in a flow chamber at variable shear stress (0.5 12.0 dynes/cm 2 ). Microbubble attachment aortic plaque...
Background— Noninvasive methods for characterizing neovessel formation during angiogenesis are currently lacking. We hypothesized that could be imaged with the use of contrast-enhanced ultrasound (CEU) microbubbles targeted to α v -integrins. Methods and Results— Microbubbles -integrins were prepared by conjugating echistatin (MB E ) or monoclonal antibody against murine their surface. Control c also prepared. The microvascular behavior these was assessed intravital microscopy cremaster...
We examined the effects of inhibiting nitric oxide synthase with N ω -nitro-l-arginine-methyl ester (l-NAME) on total hindlimb blood flow, muscle microvascular recruitment, and glucose uptake during euglycemic hyperinsulinemia in vivo rat. used two independent methods to measure perfusion. In one group animals, recruitment was measured using metabolism exogenously infused 1-methylxanthine (1-MX), a second contrast-enhanced ultrasound (CEU) used. Limb by arterial-venous concentration...
We have previously shown that skeletal muscle capillaries are rapidly recruited by physiological doses of insulin in both humans and animals. This facilitates glucose delivery to muscle, thus augmenting uptake. In obese rats, insulin-mediated microvascular recruitment uptake diminished; however, this action has not been studied humans. Here we used contrast ultrasound measure blood volume (MBV) (an index recruitment) the forearm flexor muscles lean adults before after a 120-min...
Background —Lipid microbubbles used for perfusion imaging with ultrasound are retained within inflamed tissue because of complement-mediated attachment to leukocytes venules. We hypothesized that incorporation phosphatidylserine (PS) into the microbubble shell may enhance these interactions by amplifying complement activation and thereby allow inflammation. Methods Results —In 6 mice, intravital microscopy necrosis factor-α–treated cremaster muscle was performed assess microvascular behavior...
Abstract Endothelial surface expression of P-selectin and subsequent leukocyte rolling in venules can be induced by mast cell-derived histamine binding thrombin to protease-activated receptor-1 (PAR1). We hypothesized that activation endothelial PAR2 cell tryptase or other proteases also contributes inflammatory responses. Leukocyte flux velocity were assessed intravital microscopy the cremaster muscles wild-type mice following perivenular micropipette injections a control (LSIGRL)...
Despite intensive study, the relation between insulin’s action on blood flow and glucose metabolism remains unclear. Insulin-induced changes in microvascular perfusion, independent from effects total flow, could be an important variable contributing to metabolic action. We hypothesized that modest, physiologic increments plasma insulin concentration alter perfusion human skeletal muscle these can assessed using contrast-enhanced ultrasound (CEU), a validated method for quantifying by...
Background —Albumin microbubbles that are used for contrast echocardiography persist within the myocardial microcirculation after ischemia/reperfusion (I-R). The mechanism responsible this phenomenon is unknown. Methods and Results —Intravital microscopy of exteriorized cremaster muscle was performed in 12 wild-type mice during intravenous injections fluorescein-labeled composed albumin, anionic lipids, or cationic lipids. Injections were at baseline 30 to 90 minutes I-R 8 2 hours...
Background —We have previously shown that microbubbles adhere to leukocytes in regions of inflammation. We hypothesized these are phagocytosed by neutrophils and monocytes remain acoustically active, permitting their detection inflamed tissue. Methods Results —In vitro studies were performed which activated incubated with albumin or lipid observed under microscopy. Microbubbles attached the surface monocytes, phagocytosed, remained intact for up 30 minutes. The rate destruction on exposure...