A5025387322- Atherosclerosis and Cardiovascular Diseases
- Single-cell and spatial transcriptomics
- Lymphatic System and Diseases
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Antioxidant Activity and Oxidative Stress
- Lipoproteins and Cardiovascular Health
- Birth, Development, and Health
- Proteoglycans and glycosaminoglycans research
- Plant Reproductive Biology
- Identification and Quantification in Food
- Genetically Modified Organisms Research
- Cancer, Lipids, and Metabolism
- Photosynthetic Processes and Mechanisms
- Biomarkers in Disease Mechanisms
- Aortic Disease and Treatment Approaches
- Angiogenesis and VEGF in Cancer
- Bioinformatics and Genomic Networks
- Potato Plant Research
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Biomedical Ethics and Regulation
- Protease and Inhibitor Mechanisms
- Pluripotent Stem Cells Research
Emory University
2024
Atlanta VA Health Care System
2024
University of Virginia
2018-2022
Victor Chang Cardiac Research Institute
2021
Institute of Plant Genetics, Polish Academy of Sciences
2015
Cornell University
2011
Robert W. Holley Center for Agriculture & Health
2011
Rupture and erosion of advanced atherosclerotic lesions with a resultant myocardial infarction or stroke are the leading worldwide cause death. However, we have limited understanding identity, origin, function many cells that make up late-stage lesions, as well mechanisms by which they control plaque stability.
Carotenoids are crucial for plant growth and human health. The finding of ORANGE (OR) protein as a pivotal regulator carotenogenesis offers unique opportunity to comprehensively understand the regulatory mechanisms carotenoid accumulation develop crops with enhanced nutritional quality. Here, we demonstrated that alteration single amino acid in wild-type OR greatly its ability promote accumulation. Whereas overexpression from Arabidopsis (Arabidopsis thaliana; AtOR) or agronomically...
Although sex differences in coronary artery disease are widely accepted with women developing more stable atherosclerosis than men, the underlying pathobiology of such remains largely unknown. In disease, recent integrative systems biological studies have inferred gene regulatory networks (GRNs). Within these GRNs, key driver genes shown great promise but thus far been unidentified women.We generated sex-specific GRNs atherosclerotic arterial wall 160 and age-matched men STARNET study...
Cold-induced sweetening in potato tubers is a costly problem for the food industry. To systematically identify proteins associated with this process, we employed comparative proteomics approach using isobaric, stable isotope coded labels to compare proteomes of after 0 and 5 months storage at °C. We evaluated both high pH reverse phase (hpRP) liquid chromatography (LC) off-gel electrophoresis (OGE) as first dimension fractionation methods followed by nanoLC–MS/MS, two performance mass...
The atherosclerotic plaque microenvironment is highly complex, and selective agents that modulate stability are not yet available. We sought to develop a scRNA-seq analysis workflow investigate this environment uncover potential therapeutic approaches. designed user-friendly, reproducible will be applicable other disease-specific datasets.Here we incorporated automated cell labeling, pseudotemporal ordering, ligand-receptor evaluation, drug-gene interaction into ready-to-deploy workflow....
Background: Smooth muscle cells (SMCs) in atherosclerotic plaque take on multiple nonclassical phenotypes that may affect stability and, therefore, the likelihood of myocardial infarction or stroke. However, mechanisms by which these are only beginning to be explored. Methods: In this study, we investigated contribution inflammatory MCP1 (monocyte chemoattractant protein 1) produced both classical Myh11 (myosin heavy chain 11) + SMCs and have transitioned through an Lgals3 (galectin 3) state...
Objective: Smooth muscle cells and pericytes display remarkable plasticity during injury disease progression. Here, we tested the hypothesis that perivascular give rise to Klf4 -dependent macrophage-like augment adipose tissue (AT) inflammation metabolic dysfunction associated with diet-induced obesity (DIO). Approach Results: Using Myh11-Cre ERT2 eYFP (enhanced yellow fluorescent protein) mice flow cytometry of stromovascular fraction epididymal AT, observed a large smooth lineage traced +...
Rationale: Plaque instability remains poorly understood and new therapeutic approaches to reduce plaque rupture subsequent clinical events are of great interest. Recent studies revealed an important role phenotypic switching smooth muscle cells (SMC) in controlling stability, including ECM (extracellular matrix) deposition. Objective: The aim this study was elucidate the hyaluronan derived from SMC–hyaluronan synthase 3 ( Has3 ), stability animal model atherosclerosis. Methods Results: A...
Induced pluripotent stem cells (iPSCs) directed to endothelial identity (iPSC-ECs) are emerging as a potent tool for regenerative medicine in vascular disease. However, iPSC-ECs lose expression of key markers under standard vitro conditions, limiting their clinical applications. In order model physiologic vivo we examined the bioenergetics, presence cell markers, and proliferative angiogenic capacity at late early passage hyperoxic (21%) (4%) oxygen concentration. Physoxia resulted relative...
Background/Objective: Lymphedema is a condition characterized by impaired flow of interstitial fluid through the lymphatic system and chronic obliteration distal collecting vessels. Despite advances in our knowledge development vessels, there has been little progress translating this into clinically relevant regenerative therapies. Although endothelial cells (LECs) share lineage with arterial venous cell progenitors, currently described processes to derive LECs from stem vitro take up month,...
Introduction: Induced pluripotent stem cells (iPSCs) derived endothelial (ECs) have emerged as a novel treatment strategy to restore limb perfusion in murine model of peripheral artery disease (PAD). However, our lab has demonstrated that iPSC-EC culture experience failure mitophagy and loss cellular identity, limiting therapeutic implementation. Metabolic regulation this mechanism is unknown. Methods: y6 human iPSCs were differentiated previously described (Patsch Nat Cell Bio 2015)...
Smooth muscle cells (SMC) are conventionally thought to promote atherosclerotic plaque stability by investing within the fibrous cap and protecting blood flow from harmful inflammatory core. However, lineage tracing studies have shown that SMC in can undergo a Klf4-dependent transition vivo macrophage-like state, characterized loss of markers expression multiple macrophage markers, lipid accumulation, phagocytic activity. Of major interest, induced state vitro cholesterol loading also show...