A5066344731- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Antioxidant Activity and Oxidative Stress
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Postharvest Quality and Shelf Life Management
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Banana Cultivation and Research
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Horticultural and Viticultural Research
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Phytochemicals and Antioxidant Activities
- Plant and animal studies
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Genetic diversity and population structure
- Plant Surface Properties and Treatments
- Plant nutrient uptake and metabolism
Huazhong Agricultural University
2016-2025
Shanghai Zhangjiang Laboratory
2021-2025
Citrus Research Institute
1993-2022
Ministry of Agriculture and Rural Affairs
2021
Ministry of Education of the People's Republic of China
2010-2021
Yunnan Academy of Agricultural Sciences
2020
Peng Huanwu Center for Fundamental Theory
2008
Guangdong Ocean University
2007
Lishui Academy of Forestry
2007
Institute of Crop Science
2007
Yijun Ruan and colleagues report the draft genome of sweet orange, Citrus sinensis. Their data suggests orange originated from a cross between pummelo mandarin. Oranges are an important nutritional source for human health have immense economic value. Here we present comprehensive analysis (Citrus sinensis). The assembled sequence covers 87.3% estimated genome, which is relatively compact, as 20% composed repetitive elements. We predicted 29,445 protein-coding genes, half in heterozygous...
The emergence of apomixis-the transition from sexual to asexual reproduction-is a prominent feature modern citrus. Here we de novo sequenced and comprehensively studied the genomes four representative citrus species. Additionally, 100 accessions primitive, wild cultivated Comparative population analysis suggested that genomic regions harboring energy- reproduction-associated genes are probably under selection in We also narrowed genetic locus responsible for polyembryony, form apomixis, an...
Although remarkable progress has been made toward understanding carotenoid biosynthesis, the mechanisms that regulate transcription of carotenogenic genes remain poorly understood. Lycopene β-cyclases (LCYb) are critical enzymes located at branch point biosynthetic pathway. Here, we used promoter sequence LCYb1 as bait in a yeast one-hybrid screen for promoter-binding proteins from sweet orange (Citrus sinensis). This identified MADS factor, CsMADS6, was coordinately expressed with fruit...
Flavonoids are widely distributed in plants and play important roles many biological processes. Citrus fruits rich dietary sources of flavonoids. However, there have been very few reports about the comprehensive metabolic profile natural diversity flavonoids different tissues various cultivars. In this study, based on 7416 signals detected with non-targeted metabolomics approach, Principal Component Analysis revealed flavedo has largest differences from other metabolite levels; as 198...
Although the functions of carotenogenic genes are well documented, little is known about mechanisms that regulate their expression, especially those involved in α - and β-branch carotenoid metabolism. In this study, an R2R3-MYB transcriptional factor (CrMYB68) directly regulates transformation α- carotenoids was identified using Green Ougan (MT), a stay-green mutant Citrus reticulata cv Suavissima. A comprehensive analysis developing harvested fruits indicated reduced expression β-carotene...
Abstract Chlorophyll degradation and carotenoid biosynthesis, which occur almost simultaneously during fruit ripening, are essential for the coloration nutritional value of fruits. However, synergistic regulation these 2 processes at transcriptional level remains largely unknown. In this study, we identified a WRKY transcription factor, CrWRKY42, from transcriptome data yellowish bud mutant “Jinlegan” ([Citrus unshiu × C. sinensis] reticulata) tangor its wild-type “Shiranui” tangor, was...
Secretory structures in terrestrial plants serve as reservoirs for a variety of secondary metabolites. Among these, the secretory cavity Rutaceae family is notable containing essential oils with wide range applications. However, molecular basis underlying development unknown. Here, we reveal framework Citrus oil gland formation. Using genetic mapping and genome editing, demonstrated that this process requires LATE MERISTEM IDENTITY1 (LMI1), key regulator leaf serration. A conserved GCC box...
A novel, pleiotropic sweet orange (Citrus sinensis L. Osbeck) mutant, ‘Hong Anliu’, is described. This mutation causes carotenoid accumulation, high sugar, and low acid in the fruits. Gas chromatographic analysis revealed that sugar fruit were caused by accumulation of sucrose deficiency citric acid. The dominant accumulated albedo, segment membranes, juice sacs lycopene, which can reach levels are a 1000-fold higher than those comparable wild-type does not affect composition leaves....
Abstract Background From field harvest to the consumer’s table, fresh citrus fruit spends a considerable amount of time in shipment and storage. During these processes, physiological disorders pathological diseases are main causes loss. Heat treatment (HT) has been widely used maintain quality during postharvest storage; however, limited molecular information related this is currently available at systemic biological level. Results Mature ‘Kamei’ Satsuma mandarin ( Citrus unshiu Marc.)...
Abstract Background The transcriptome of the fruit pulp sweet orange variety Anliu (WT) and that its red fleshed mutant Hong (MT) were compared to understand dynamics differential expression genes expressed during development ripening. Results transcriptomes WT MT sampled at four developmental stages using an Illumina sequencing platform. A total 19,440 18,829 detected in WT, respectively. Hierarchical clustering analysis revealed 24 patterns for set all detected, which 20 common between WT....
Fruit ripening is a complex, genetically programmed process that occurs in conjunction with the differentiation of chloroplasts into chromoplasts and involves changes to organoleptic properties fruit. In this study, an integrative analysis transcriptome proteome was performed identify important regulators pathways involved fruit spontaneous late-ripening mutant (‘Fengwan’ orange, Citrus sinensis L. Osbeck) its wild type (‘Fengjie 72-1’). At transcript level, 628 genes showed 2-fold or more...
Red-flesh fruit is absent from common sweet orange varieties, but more preferred by consumers due to its visual attraction and nutritional properties. Our previous researches on a spontaneous red-flesh mutant revealed that the trait caused lycopene accumulation regulated both transcriptional post-transcriptional mechanisms. However, knowledge regulation of in fruits rather limited so far. We used Illumina sequencing method identify quantitatively profile small RNAs wild type. identified 85...
Abstract Flavonols and hydroxycinnamic acids are important phenylpropanoid metabolites in plants. In this study, we isolated characterized a citrus R2R3-MYB transcription factor CsMYBF1 , encoding protein belonging to the flavonol-specific MYB subgroup. Ectopic expression of tomato led an up-regulation series genes involved primary metabolism pathway, induced strong accumulation acid compounds but not flavonols. The RNAi suppression callus caused down-regulation many pathway reduced contents...
Green and blue mold decay, caused by Penicillium digitatum P. italicum, respectively, are important postharvest diseases of citrus. Biocontrol microbes is an alternative to synthetic fungicide application. In this study, the antagonistic yeast strain Kloeckera apiculata 34-9 was used investigate action mechanisms involved in biocontrol diseases.An antifungal substance, 2-phenylethanol (PEA), isolated from K. demonstrated have antimicrobial activity against selected phytopathogenic fungi....