- GABA and Rice Research
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- Food composition and properties
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Aluminum toxicity and tolerance in plants and animals
- Spectroscopy and Chemometric Analyses
- Plant responses to water stress
- Plant Disease Resistance and Genetics
- Biofuel production and bioconversion
- Plant Reproductive Biology
- Food Quality and Safety Studies
- Plant Micronutrient Interactions and Effects
- Biochemical and biochemical processes
- Plant tissue culture and regeneration
- Heavy metals in environment
- Medicinal Plants and Neuroprotection
- Polysaccharides Composition and Applications
- Agriculture, Soil, Plant Science
- Genomics and Phylogenetic Studies
China National Rice Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2010-2025
Zhejiang Lab
2023-2024
Shenyang Agricultural University
2019-2024
Jiangxi Agricultural University
2015-2024
Zhejiang University
2004-2024
Rice Research Institute
2019-2024
Ministry of Agriculture and Rural Affairs
2022
Hunan Agricultural University
2018-2019
State Key Laboratory of Rice Biology
2015-2016
Abstract The three most important agronomic traits of rice (Oryza sativa), yield, plant height, and flowering time, are controlled by many quantitative trait loci (QTLs). In this study, a newly identified QTL, DTH8 (QTL for days to heading on chromosome 8), was found regulate these in rice. Map-based cloning reveals that encodes putative HAP3 subunit the CCAAT-box-binding transcription factor complementary experiment increased significantly heading, number grains per panicle CSSL61 (a...
Tre6P (trehalose-6-phosphate) mediates sensing of carbon availability to maintain sugar homeostasis in plants, which underpins crop yield and resilience. However, how responds fluctuations levels regulates the utilization sugars for growth remains be addressed. Here, we report that sugar-inducible rice NAC transcription factor OsNAC23 directly represses phosphatase gene TPP1 simultaneously elevate repress trehalose levels, thus facilitating partitioning from source sink organs. Meanwhile,...
Rice grain filling determines weight, final yield and quality. Here, a rice defective mutant, gif2, was identified. Grains of gif2 showed slower rate significant lower weight compared to wild-type. The starch content in noticeably decreased its physicochemical properties were also altered. Moreover, endosperm cells obvious defects compound granule formation. Positional cloning identified GIF2 encode an ADP-glucose pyrophosphorylase (AGP) large subunit, AGPL2; consequently, AGP enzyme...
Two-line hybrid rice with high yield potential is increasingly popular and the photo- temperature-sensitive male sterile line one of basic components for two-line breeding. The development lines through conventional breeding a lengthy laborious process, whereas developing thermo-sensitive genic (TGMS) by editing temperature-sensitivity gene CRISPR/Cas9 efficient convenient. Here, sterility was induced employing technology to modify TMS5. Two TGMS mutants, tms5–1 tms5–2, both lacking any...
Rice, being a major staple food crop and sensitive to salinity conditions, bears heavy yield losses due saline soil. Although some salt responsive genes have been identified in rice, their applications developing tolerant cultivars resulted limited achievements. Herein, we used bioinformatic approaches perform meta-analysis of three transcriptome datasets from control conditions order reveal novel the molecular pathways underlying rice response salt. From total 28,432 expressed genes,...
Summary Starch is the main form of energy storage in higher plants. Although several enzymes and regulators starch biosynthesis have been defined, complete molecular machinery remains largely unknown. Screening for irregularities endosperm formation rice represents valuable prospect studying synthesis pathway. Here, we identified a novel white‐core defective grain filling mutant, ospk2 , which displays significantly lower weight, decreased content alteration physicochemical properties when...
Under conditions of labor or resource scarcity, direct seeding, rather than transplantation, is the preferred mode rice (Oryza sativa) cultivation. This approach requires varieties that exhibit uniform seedling emergence. Mesocotyl elongation (ME), main driver rapid emergence seedlings from soil, enhanced by darkness and inhibited light. Plant polyamine oxidases (PAOs) oxidize polyamines (PAs) release H2O2. Here, we established OsPAO5 expression in increased presence light darkness. To...
Aroma is a key grain quality trait that directly influences the market price of rice globally. Loss function betaine aldehyde dehydrogenase 2 (OsBADH2) affects biosynthesis 2-acetyl-1-pyrroline (2-AP), which responsible for aroma in fragrant rice. The current study was aimed at creating new alleles BADH2 using CRISPR/Cas9 gene editing technology under genetic background japonica Ningjing 1 (NJ1) and indica Huang Huazhan (HHZ) varieties. Sensory evaluation analysis headspace solid-phase...
Abstract Preharvest sprouting (PHS) is a deleterious phenotype that occurs frequently in rice-growing regions where the temperature and precipitation are high. It negatively affects yield, quality, downstream grain processing. Seed dormancy trait related to PHS. Longer seed preferred for rice production as it can prevent Here, we map QTLs associated with clone Dormancy 3.1 ( SDR3.1 ) underlying one major QTL. encodes mediator of OsbZIP46 deactivation degradation (MODD). We show regulates by...
Transitory starch is an important carbon source in leaves, and its biosynthesis metabolism are closely related to grain quality yield. The molecular mechanisms controlling leaf transitory degradation their effects on rice (Oryza sativa) yield remain unclear. Here, we show that OsLESV OsESV1, the orthologs of AtLESV AtESV1, associated with rice. total amylose contents leaves endosperms significantly reduced, final compromised oslesv osesv1 single esv1 double mutants. Furthermore, found OsESV1...
Abstract MicroRNAs (miRNAs) play fundamental roles in many developmental and physiological processes eukaryotes. MiRNAs plants generally regulate their targets via either mRNA cleavage or translation repression; however, which approach plays a major role whether these two function modes can shift remains elusive. Here, we identify miRNA, miR408-5p that regulates AUXIN/INDOLE ACETIC ACID 30 ( IAA30 ), critical repressor the auxin pathway switching action rice. We find usually inhibits protein...
Summary Rice ( Oryza sativa L.) is one of the most important food crops. Starch main substance rice endosperm and largely determines grain quality yield. biosynthesis in very complex, requiring a series enzymes which are also regulated by many transcription factors (TFs). But until now, large‐scale regulatory network for starch has not been established. Here, we constructed comprised 277 TFs 15 synthesis enzyme‐encoding genes (SSEGs) using DNA affinity chromatography/pull‐down combined with...
Isoamylase 1 (ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease 9 (CRISPR/Cas9) system was used to edit ISA1 gene rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T0 transgenic events, and classified mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1 (type...
Abstract The percentage of amylose in the endosperm rice ( Oryza sativa ) largely determines grain cooking and eating qualities. Granule‐bound starch synthase I (GBSSI) GBSSII are responsible for biosynthesis leaf, respectively. Here, we identified OsGBP , a GBSS‐binding protein that interacted with GBSSI vitro vivo . total contents osgbp mutants were significantly lower than those wild type leaves grains, resulting reduced weight quality. carbohydrate‐binding module 48 (CBM48) domain...
Starch and storage proteins are the main components of rice (Oryza sativa L.) grains. Despite their importance, molecular regulatory mechanisms protein starch biosynthesis remain largely elusive. Here, we identified a opaque endosperm mutant, opaque3 (o3), that overaccumulates 57-kDa proglutelins has significantly lower contents than wild type. The o3 mutant also abnormal body structures compound grains in its cells. OPAQUE3 (O3) encodes transmembrane basic leucine zipper (bZIP)...
Due to anthropogenic activities, environmental pollution of heavy metals/metalloids (HMs) has increased and received growing attention in recent decades. Plants HM-contaminated soils have slower growth development, resulting lower agricultural yield. Exposure HMs leads the generation free radicals (oxidative stress), which alters plant morpho-physiological biochemical pathways at cellular tissue levels. evolved complex defense mechanisms avoid or tolerate toxic effects HMs, including...
Head rice yield (HRY) measures milling quality and determines final grain commercial value. Here, we report that two major quantitative trait loci for in rice, qMq-1 qMq-2, represent allelic variants of Waxy
ABSTRACT A mechanized direct seeding of rice with less labor and water usage, has been widely adopted. However, this approach requires varieties that exhibit uniform seedling emergence. Mesocotyl elongation (ME) offers the main drive fast emergence seedlings from soils; nevertheless, its genetic basis remains unknown. Here, we identify a major quantitative trait locus Elongation1 ( qME1 ), an allele Green Revolution gene Semi‐Dwarf1 SD1 encoding GA20‐oxidase for gibberellin (GA) biosynthesis...