A5101421581- GABA and Rice Research
- Food composition and properties
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- Rice Cultivation and Yield Improvement
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Biofuel production and bioconversion
- Spectroscopy and Chemometric Analyses
- Plant Virus Research Studies
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Phytase and its Applications
- Aluminum toxicity and tolerance in plants and animals
- Enzyme Production and Characterization
- Plant and Fungal Species Descriptions
- Microbial Metabolites in Food Biotechnology
- Heavy Metal Exposure and Toxicity
- Seed Germination and Physiology
China National Rice Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2025
Ministry of Agriculture and Rural Affairs
2022
Hunan Agricultural University
2019
State Key Laboratory of Rice Biology
2015-2016
Rice University
2013
China Agricultural University
2007
Rice grain filling determines weight, final yield and quality. Here, a rice defective mutant, gif2, was identified. Grains of gif2 showed slower rate significant lower weight compared to wild-type. The starch content in noticeably decreased its physicochemical properties were also altered. Moreover, endosperm cells obvious defects compound granule formation. Positional cloning identified GIF2 encode an ADP-glucose pyrophosphorylase (AGP) large subunit, AGPL2; consequently, AGP enzyme...
Two-line hybrid rice with high yield potential is increasingly popular and the photo- temperature-sensitive male sterile line one of basic components for two-line breeding. The development lines through conventional breeding a lengthy laborious process, whereas developing thermo-sensitive genic (TGMS) by editing temperature-sensitivity gene CRISPR/Cas9 efficient convenient. Here, sterility was induced employing technology to modify TMS5. Two TGMS mutants, tms5–1 tms5–2, both lacking any...
Summary Starch is the main form of energy storage in higher plants. Although several enzymes and regulators starch biosynthesis have been defined, complete molecular machinery remains largely unknown. Screening for irregularities endosperm formation rice represents valuable prospect studying synthesis pathway. Here, we identified a novel white‐core defective grain filling mutant, ospk2 , which displays significantly lower weight, decreased content alteration physicochemical properties when...
Rice flowering is a major agronomic trait, determining yield and ecological adaptability in particular regions. ABA plays an essential role rice flowering, but the underlying molecular mechanism remains largely elusive. In this study, we demonstrated "SAPK8-ABF1-Ehd1/Ehd2" pathway, through which exogenous represses photoperiod-independent manner. We generated abf1 sapk8 mutants using CRISPR-Cas9 method. Using yeast two-hybrid, Pull down, BiFC kinase assays, SAPK8 interacted phosphorylated...
Head rice yield (HRY) measures milling quality and determines final grain commercial value. Here, we report that two major quantitative trait loci for in rice, qMq-1 qMq-2, represent allelic variants of Waxy
Isoamylase 1 (ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease 9 (CRISPR/Cas9) system was used to edit ISA1 gene rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T0 transgenic events, and classified mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1 (type...
Abstract Obtaining genetic variation information from indica rice hybrid parents and identification of loci associated with heterosis are important for breeding. Here, we resequence 1,143 accessions mostly selected the superior cultivars China, identify variations, perform kinship analysis. We find different crossing patterns between 3- 2-line lines. By calculating frequencies parental differences (FPVDs), a more direct approach studying heterosis, that linked to which include 98 in 3-line...
Abstract Starch is the main storage carbohydrate in higher plants. Although several enzymes and regulators for starch biosynthesis have been characterized, a complete regulatory network synthesis cereal seeds remains elusive. Here, we report identification characterization of rice Brittle1 (OsBT1 ) gene, which expressed specifically developing endosperm. The osbt1 mutant showed white-core endosperm significantly lower grain weight than wild-type. formation development compound granules was...
Abstract The percentage of amylose in the endosperm rice ( Oryza sativa ) largely determines grain cooking and eating qualities. Granule‐bound starch synthase I (GBSSI) GBSSII are responsible for biosynthesis leaf, respectively. Here, we identified OsGBP , a GBSS‐binding protein that interacted with GBSSI vitro vivo . total contents osgbp mutants were significantly lower than those wild type leaves grains, resulting reduced weight quality. carbohydrate‐binding module 48 (CBM48) domain...
Starch and storage proteins are the main components of rice (Oryza sativa L.) grains. Despite their importance, molecular regulatory mechanisms protein starch biosynthesis remain largely elusive. Here, we identified a opaque endosperm mutant, opaque3 (o3), that overaccumulates 57-kDa proglutelins has significantly lower contents than wild type. The o3 mutant also abnormal body structures compound grains in its cells. OPAQUE3 (O3) encodes transmembrane basic leucine zipper (bZIP)...
The plastid-encoded RNA polymerase (PEP) plays an important role in the transcription machinery of mature chloroplasts, yet details its function remain elusive rice. Here, we identified a novel PEP-associated protein (PAP), WLP2, based on two allelic white leaf and panicle mutants, wlp2s wlp2w. mutants were albino lethal at high temperatures showed decreased chlorophyll accumulation, abnormal chloroplast ultrastructure, attenuated photosynthetic activity. Map-based cloning suggested that...
The environmental damage caused by cadmium (Cd) pollution is of increasing concern in China. While the overall plant response to Cd has been investigated some depth, contribution (if any) protein phosphorylation detoxification and expression tolerance uncertain. Here, molecular basis explored hydroponically raised rice seedlings exposed 10 μΜ 100 Cd2+ stress. An analysis seedlings' quantitative phosphoproteome identified 2454 phosphosites, associated with 1244 proteins. A total 482 these...
Pre-harvest sprouting (PHS) is a disadvantageous trait in cereal production worldwide, causing large economic losses each year. Its regulation mechanism still unclear. We generated the Oryza sativa Viviparous1 (OsVP1) mutant using gene editing technique, which shows increased PHS compared with that of wild type Nipponbare. OsVP1 localized mainly nucleus and expressed various tissues organs. Expression Seed dormancy 4 (Sdr4), key controlling PHS, was sharply reduced mutants. bound to specific...
Pentatricopeptide repeat (PPR) proteins play important roles in the post-transcriptional modification of organellar RNAs plants. However, function most PPR remains unknown. Here, we characterized rice (Oryza sativa L.) chlorophyll deficient 4 (cde4) mutant which exhibits an albino phenotype during early leaf development, with decreased contents and abnormal chloroplasts at low-temperature (20°C). Positional cloning revealed that CDE4 encodes a P-type protein localized chloroplasts. In cde4...
Polycomb group (PcG) proteins have been shown to affect growth and development in plants. To further elucidate their role these processes rice, we isolated characterized a rice mutant which exhibits dwarfism, reduced seed setting rate, defective floral organ, small grains. Map-based cloning revealed that abnormal phenotypes were attributed mutation of the Fertilization Independent Endosperm 2 (OsFIE2) protein, belongs PcG protein family. So named as osfie2-1. Histological analysis number...