A5027796558- Malaria Research and Control
- Metabolomics and Mass Spectrometry Studies
- Mosquito-borne diseases and control
- Mass Spectrometry Techniques and Applications
- Complement system in diseases
- Bacterial biofilms and quorum sensing
- Invertebrate Immune Response Mechanisms
- Long-Term Effects of COVID-19
- Antibiotic Resistance in Bacteria
- Glycosylation and Glycoproteins Research
- Microbial Community Ecology and Physiology
- Spectroscopy Techniques in Biomedical and Chemical Research
- SARS-CoV-2 and COVID-19 Research
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Advanced Proteomics Techniques and Applications
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- HIV Research and Treatment
- Polyomavirus and related diseases
- Redox biology and oxidative stress
- Osteoarthritis Treatment and Mechanisms
- Immunotherapy and Immune Responses
University of Florida
2019-2023
Montana State University
2013-2022
Johns Hopkins University
2019
Bacteria in biofilms are protected from killing by antibiotics, and this reduced susceptibility contributes to the persistence of infections such as those cystic fibrosis lung chronic wounds. A generalized conceptual model biofilm antimicrobial tolerance with following mechanistic steps is proposed: (i) establishment concentration gradients metabolic substrates products; (ii) active biological responses these changes local chemical microenvironment; (iii) entry cells into a spectrum states...
A noninvasive malaria rapid test assesses subclinical carriage of Plasmodium falciparum (human malaria) parasites at the point need.
Abstract An obligatory step in the complex life cycle of malaria parasite is sporogony, which occurs during oocyst stage adult female Anopheles mosquitoes. Sporogony metabolically demanding, and successful maturation dependent on host lipids. In insects, lipid energy reserves are mobilized by adipokinetic hormones (AKHs). We hypothesized that Plasmodium falciparum infection activates gambiae AKH signaling mobilization. profiled expression patterns pathway genes Ag Akh1 peptide levels An....
Leishmania donovani infection of macrophages results in quantitative and qualitative changes the protein profile extracellular vesicles (EVs) released by infected host cells. We confirmed mass spectrometry orthogonally performing Western blots for several -infected macrophage-enriched EVs (LieEVs) molecules. Several cell proteins LieEVs have been implicated promoting vascular other systems. also identified 59 parasite-derived LieEVs, including a putative L. homolog mammalian vasohibins...
Lipids composed of condensed isoprenyl units connected to glycerol backbones by ether linkages are a distinguishing feature Archaea. Data suggesting that fatty acids with linear hydrocarbon chains present in some Archaea have been available for decades. However, lack genomic and biochemical evidence the metabolic machinery required synthesize degrade has left field unclear on this potentially significant aspect. Because lipids energy currency cell signaling molecules, their presence is...
In vitro studies of liver stage (LS) development the human malaria parasite Plasmodium falciparum are technically challenging; therefore, fundamental questions about hepatocyte receptors for invasion that can be targeted to prevent infection remain unanswered. To identify novel and further understand susceptibility P. sporozoite invasion, we created an optimized in system by mimicking vivo conditions using subcloned HC-04.J7 cell line supports mean rates 3-5% early exoerythrocytic forms—a 3-...
Abstract Malaria transmission-blocking vaccines (TBVs) prevent the completion of developmental lifecycle malarial parasites within mosquito vector, effectively blocking subsequent infections. The midgut protein Anopheline alanyl aminopeptidase N (AnAPN1) is leading, mosquito-based TBV antigen. Structure-function studies identified two Class II epitopes that can induce potent (T-B) antibodies, informing design next-generation AnAPN1. Here, we functionally screened new immunogens and...
Thrombospondin type I repeat (TSR) domains are commonly O-fucosylated by protein O-fucosyltransferase 2 (PoFUT2), and this modification is required for optimal folding secretion of TSR-containing proteins. The human malaria parasite Plasmodium falciparum expresses proteins containing TSR domains, such as the thrombospondin-related anonymous (TRAP) circumsporozoite surface (CSP), which O-fucosylated. TRAP CSP present on sporozoites play essential roles in mosquito host invasion processes...
Malaria is a major global health threat, with nearly half the world's population at risk of infection. Given recently described delayed clearance parasites by artemisinin-combined therapies, new antimalarials are needed to facilitate effort toward elimination and eradication. NPC1161 an 8-aminoquinoline that derived from primaquine improved therapeutic profile compared parent compound. The (R)-(-) enantiomer (NPC1161B) has lower effective dose results in decreased toxic side effects such as...
SARS-CoV-2 subverts host cell processes to facilitate rapid replication and dissemination, this leads pathological inflammation.We used niclosamide (NIC), a poorly soluble anti-helminth drug identified initially for repurposed treatment of COVID-19, which activates the cells' autophagic lipophagic as chemical probe determine if it can modulate cell's total lipid profile that would otherwise be either amplified or reduced during infection.Through parallel lipidomic transcriptomic analyses we...
SUMMARY The global effort to combat COVID-19 rapidly produced a shortlist of approved drugs with anti-viral activities for clinical repurposing. However, the jump testing was lethal in some cases as full understanding mechanism antiviral activity opposed pleiotropic activity/toxicity these lacking. Through parallel lipidomic and transcriptomic analyses we observed massive reorganization lipid profiles infected Vero E6 cells, especially plasmalogens that correlated increased levels virus...
Is there a universal genetically programmed defense providing tolerance to antibiotics when bacteria grow as biofilms? A comparison between biofilms of three different bacterial species by transcriptomic and metabolomic approaches uncovered no evidence one. Single-species (Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii) were grown
A successful malaria transmission blocking vaccine (TBV) requires the induction of a high antibody titer that leads to abrogation parasite traversal mosquito midgut following ingestion an infectious bloodmeal, thereby cascade secondary human infections. Previously, we developed optimized construct UF6b elicits antigen-specific response neutralizing epitope Anopheline alanyl aminopeptidase N (AnAPN1), evolutionarily conserved pan-malaria midgut-based TBV target, as well established...
The ability to generate two-dimensional images of a wound that contains information about the distribution bacteria overlaid with drugs and metabolites could enhance our understanding healing processes. Advances in technology are leading rapid expansion mass spectrometry-based imaging. When combined matrix assisted laser desorption ionization ionize wide range molecules, imaging spectrometry is powerful biomedical research tool. However, this technique has yet be used investigate bacterial...