A5031006470- Malaria Research and Control
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Computational Drug Discovery Methods
- COVID-19 Impact on Reproduction
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Drug Transport and Resistance Mechanisms
- Neural dynamics and brain function
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Parasites and Host Interactions
- Lipoproteins and Cardiovascular Health
- Crystallization and Solubility Studies
- Iron Metabolism and Disorders
- HIV/AIDS drug development and treatment
- Advanced Battery Technologies Research
- X-ray Diffraction in Crystallography
- HIV/AIDS Research and Interventions
- Research on Leishmaniasis Studies
- Viral Infections and Vectors
- Global Maternal and Child Health
- HIV Research and Treatment
Johns Hopkins University
2016-2025
Bangor University
2008-2025
Midwestern University
2025
Northwestern University
2025
Royal Prince Alfred Hospital
1997-2024
Allen Institute
2018-2024
Johns Hopkins Medicine
2006-2024
The University of Sydney
2007-2024
Texas A&M University
2011-2024
The University of Texas at Arlington
2024
Chloroquine is thought to exert its antimalarial effect by preventing the polymerization of toxic heme released during proteolysis hemoglobin in Plasmodium digestive vacuole. The mechanism this blockade has not been established. We incubated cultured parasites with subinhibitory doses [3H]chloroquine and [3H] quinidine. These [3H]quinoline compounds became associated hemozoin as assessed electron microscope autoradiography subcellular fractionation. In vitro, binding inhibitors chain...
Convalescent plasma is a leading treatment for coronavirus disease 2019 (COVID-19), but there paucity of data identifying its therapeutic efficacy. Among 126 potential convalescent donors, the humoral immune response was evaluated using severe acute respiratory syndrome 2 (SARS–CoV-2) virus neutralization assay with Vero-E6-TMPRSS2 cells; commercial IgG and IgA ELISA to detect spike (S) protein S1 domain (EUROIMMUN); IgA, IgG, IgM indirect ELISAs full-length S or receptor–binding (S-RBD); an...
The digestive vacuole of Plasmodium falciparum is the site hemoglobin degradation, heme polymerization into crystalline hemozoin, and antimalarial drug accumulation. Antibodies identified histidine-rich protein II (HRP II) in purified vacuoles. Recombinant or native HRP promoted formation chloroquine inhibited reaction. related III also polymerized heme, an additional was It proposed that after secretion by parasite host erythrocyte cytosol, HRPs are brought acidic along with hemoglobin....
Characterization of the T cell response in individuals who recover from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is critical to understanding its contribution protective immunity. A multiplexed peptide-MHC tetramer approach was used screen 408 SARS-CoV-2 candidate epitopes for CD8+ recognition a cross-sectional sample 30 disease 2019 convalescent individuals. cells were evaluated using 28-marker phenotypic panel, and findings modelled against time diagnosis...
Current therapy for glioblastoma multiforme is insufficient, with nearly universal recurrence. Available drug therapies are unsuccessful because they fail to penetrate through the region of brain containing tumor cells and kill most responsible development resistance, cancer stem (BCSCs). To address these challenges, we combined two major advances in technology: ( i ) brain-penetrating polymeric nanoparticles that can be loaded drugs optimized intracranial convection-enhanced delivery ii...
Polyclonal convalescent plasma may be obtained from donors who have recovered coronavirus disease 2019 (Covid-19). The efficacy of this in preventing serious complications outpatients with recent-onset Covid-19 is uncertain.
Accurate serological assays to detect antibodies severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed characterize the epidemiology of SARS-CoV-2 infection and identify potential candidates for disease 2019 (COVID-19) convalescent plasma (CCP) donation. This study compared performances commercial enzyme immunoassays (EIAs) with respect detection IgG or total neutralizing (nAbs). The diagnostic accuracy five commercially available EIAs (Abbott, Euroimmun, EDI,...
The intraerythrocytic malaria parasite constructs an intracellular haem crystal, called haemozoin, within acidic digestive vacuole where haemoglobin is degraded. Haem crystallization the target of widely used antimalarial quinoline drugs. mechanism molecular initiation crystallization, whether by proteins, polar membrane lipids or neutral lipids, has not been fully substantiated. In present study, we show lipid predominant nanospheres, which envelop haemozoin inside Plasmodium falciparum...
Angiogenesis, the formation of new blood vessels, is implicated in a number important human diseases, including cancer, diabetic retinopathy, and rheumatoid arthritis. To identify clinically useful angiogenesis inhibitors, we assembled screened library mostly Food Drug Administration-approved drugs for inhibitors endothelial cell proliferation. One most promising unexpected hits was itraconazole, known antifungal drug. Itraconazole inhibits cycle progression at G1 phase vitro blocks vascular...
Cerebral malaria (CM) is a deadly complication of Plasmodium falciparum infection, but specific interactions involved in cerebral homing infected erythrocytes (IEs) are poorly understood. In this study, P. -IEs were characterized for binding to primary human brain microvascular endothelial cells (HBMECs). Before selection, CD36 or ICAM-1–binding parasites exhibited punctate subpopulation HBMECs and was dependent. Panning IEs on led more dispersed phenotype the selection three var genes,...
An AccQ*Tag ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (AccQ*Tag-UPLC-ESI-MS/MS) method for fast, reproducible, and sensitive amino acid quantitation in biological samples, particularly, the malaria parasite Plasmodium falciparum is presented. The Waters Acquity TQD UPLC/MS system equipped with a photodiode array (PDA) detector was used separation detection. developed validated using standard mixtures containing acidic, neutral, basic acids. For...