Jérôme Lecoq
A5016337396- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Visual perception and processing mechanisms
- Olfactory and Sensory Function Studies
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Cell Image Analysis Techniques
- Functional Brain Connectivity Studies
- Advanced Chemical Sensor Technologies
- Receptor Mechanisms and Signaling
- Optical Imaging and Spectroscopy Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- CCD and CMOS Imaging Sensors
- bioluminescence and chemiluminescence research
- Gene Regulatory Network Analysis
- Blind Source Separation Techniques
- Particle Detector Development and Performance
- Photoacoustic and Ultrasonic Imaging
- Biochemical Analysis and Sensing Techniques
- Superconducting Materials and Applications
- Particle physics theoretical and experimental studies
Allen Institute
2016-2025
Allen Institute for Brain Science
2016-2025
Stanford University
2011-2022
Clark University
2011-2022
Neurophysiologie et nouvelles microscopies
2008-2011
Inserm
2007-2011
Université Paris Cité
2007-2011
Institut National de Recherche en Santé Publique
2011
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
Centre National de la Recherche Scientifique
2010
A major technological goal in neuroscience is to enable the interrogation of individual cells across live brain. By creating a curved glass replacement dorsal cranium and surgical methods for its installation, we developed chronic mouse preparation providing optical access an estimated 800,000–1,100,000 neurons surface neocortex. Post-surgical histological studies revealed comparable glial activation as control mice. In behaving mice expressing Ca2+ indicator cortical pyramidal neurons,...
Transgenic mouse lines are invaluable tools for neuroscience but, as with any technique, care must be taken to ensure that the tool itself does not unduly affect system under study. Here we report aberrant electrical activity, similar interictal spikes, and accompanying fluorescence events in some genotypes of transgenic mice expressing GCaMP6 genetically encoded calcium sensors. These epileptiform have been observed particularly, but exclusively, Emx1-Cre Ai93 transgenes, either sex, across...
Fluorescent calcium indicators are often used to investigate neural dynamics, but the relationship between fluorescence and action potentials (APs) remains unclear. Most APs can be detected when soma almost fills microscope's field of view, image populations neurons, necessitating a large generating fewer photons per neuron, compromising AP detection. Here, we characterized AP-fluorescence transfer function in vivo for 48 layer 2/3 pyramidal neurons primary visual cortex, with simultaneous...
Summary To understand how the brain processes sensory information to guide behavior, we must know stimulus representations are transformed throughout visual cortex. Here report an open, large-scale physiological survey of neural activity in awake mouse cortex: Allen Brain Observatory Visual Coding dataset. This publicly available dataset includes cortical from nearly 60,000 neurons collected 6 areas, 4 layers, and 12 transgenic lines 221 adult mice, response a systematic set stimuli. Using...
Despite advances in experimental techniques and accumulation of large datasets concerning the composition properties cortex, quantitative modeling cortical circuits under in-vivo-like conditions remains challenging. Here we report publicly release a biophysically detailed circuit model layer 4 mouse primary visual receiving thalamo-cortical inputs. The 45,000-neuron was subjected to battery stimuli, results were compared published work new vivo experiments. Simulations reproduced variety...
Vasoactive intestinal peptide-expressing (VIP) interneurons in the cortex regulate feedback inhibition of pyramidal neurons through suppression somatostatin-expressing (SST) and, reciprocally, SST inhibit VIP neurons. Although neuron activity primary visual (V1) mouse is highly correlated with locomotion, relevance locomotion-related to coding not known. Here we show that V1 respond strongly low contrast front-to-back motion congruent self-motion during locomotion but are suppressed by other...
The detection of novel stimuli is critical to learn and survive in a dynamic environment. Though powerfully affect brain activity, their impact on specific cell types circuits not well understood. Disinhibition one candidate mechanism for novelty-induced enhancements activity. Here we characterize the stimulus novelty disinhibitory circuit components using longitudinal 2-photon calcium imaging Vip, Sst, excitatory populations mouse visual cortex. Mice behavioral task with that become highly...
In the brain, neuronal activation triggers an increase in cerebral blood flow (CBF). Here, we use two animal models and several techniques (two-photon imaging of CBF calcium dynamics, intracellular extracellular recordings, local pharmacology) to analyze relationship between activity during odor stimulation rodent olfactory bulb. Application glutamate receptor antagonists or tetrodotoxin directly into single rat glomeruli blocked postsynaptic responses but did not affect odor-evoked...
Abstract The mammalian visual system, from retina to neocortex, has been extensively studied at both anatomical and functional levels. Anatomy indicates the cortico-thalamic system is hierarchical, but characterization of cellular-level interactions across multiple levels this hierarchy lacking, partially due challenge simultaneously recording activity numerous regions. Here, we describe a large, open dataset (part Allen Brain Observatory ) that surveys spiking units in six cortical two...
Abstract The mammalian cortex is a laminar structure composed of many cell types densely interconnected in complex ways. Recent systematic efforts to map the mouse mesoscale connectome provide comprehensive projection data on interareal connections, but not at level specific classes or layers within cortical areas. We present here significant expansion Allen Mouse Brain Connectivity Atlas, with ∼1,000 new axonal mapping experiments across nearly all isocortical areas 49 Cre driver lines....
Extracellular electrophysiology and two-photon calcium imaging are widely used methods for measuring physiological activity with single-cell resolution across large populations of cortical neurons. While each these two modalities has distinct advantages disadvantages, neither provides complete, unbiased information about the underlying neural population. Here, we compare evoked responses in visual cortex recorded awake mice under highly standardized conditions using either genetically...
The relationship between metabolism of neuronal activity, microvascular organization, and blood flow dynamics is critical for interpreting functional brain imaging. Here we used the rat dorsal olfactory bulb as a model to determine in vivo correlation action potential propagation, synaptic transmission, oxygen consumption, capillary density during odor stimulation. We find that lumen occupies ∼3% glomerular volume, where transmission occurs, only 0.1% overlying nerve layer. In glomeruli,...