A5069851488- Neural dynamics and brain function
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Lipid Membrane Structure and Behavior
- Circadian rhythm and melatonin
- Memory and Neural Mechanisms
- Functional Brain Connectivity Studies
- Diverse Topics in Contemporary Research
- Diverse Approaches in Healthcare and Education Studies
- Neuroscience and Neural Engineering
- Protein Kinase Regulation and GTPase Signaling
- Visual perception and processing mechanisms
- GDF15 and Related Biomarkers
- CRISPR and Genetic Engineering
- Technology and Data Analysis
- Melanoma and MAPK Pathways
- Cholinesterase and Neurodegenerative Diseases
- Bioinformatics and Genomic Networks
- Cell Adhesion Molecules Research
- Physical Activity and Education Research
- Genomics, phytochemicals, and oxidative stress
Danube Research Institute
2023
Allen Institute
2009-2020
Allen Institute for Brain Science
2009-2020
Grace (United States)
2019-2020
University of Massachusetts Chan Medical School
2005-2006
Howard Hughes Medical Institute
2005
Seoul National University
1999-2002
Sungkyunkwan University
2000
DAF-16/forkhead transcription factor, the downstream target of insulin-like signaling in Caenorhabditis elegans , is indispensable for both lifespan regulation and stress resistance. Here, we demonstrate that c-Jun N-terminal kinase (JNK) a positive regulator DAF-16 processes. Our genetic analysis suggests JNK pathway acts parallel with to regulate pathways converge onto DAF-16. We also show JNK-1 directly interacts phosphorylates Moreover, response heat stress, promotes translocation into...
Significant advances in circuit-level analyses of the brain require tools that allow for labeling, modulation gene expression, and monitoring manipulation cellular activity specific cell types and/or anatomical regions. Large-scale projects individual laboratories have produced hundreds gene-specific promoter-driven Cre mouse lines invaluable enabling genetic access to subpopulations cells brain. However, potential utility each line may not be fully realized without systematic whole...
Highlights•Anatomical characterization of Cre expression in the retina 88 driver lines•Morphological and histochemical classification Cre+ RGC types 26 lines•High resolution whole brain imaging labeled retinal axons reveals central targets•Correspondences described between line projection patternsSummaryUnderstanding how >30 ganglion cells (RGCs) mouse each contribute to visual processing will require more tools that label manipulate specific RGCs. We screened analyzed recombinase using...
ABSTRACT The connections between the claustrum and cortex in mouse are systematically investigated with adeno‐associated virus (AAV), an anterograde viral tracer. We first define boundary three‐dimensional structure of based on a variety molecular anatomical data. From AAV injections into 42 neocortical allocortical areas, we conclude that most cortical areas send bilateral projections to claustrum, majority being denser ipsilateral side. This includes prelimbic, infralimbic, medial,...
Abstract Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate curves, points regions-of-interest the space a volumetric image with single operation, such as computer mouse stroke, or click zoom from 2D-projection plane an visualized computer. VF provides efficient methods for acquisition, images roundworm, fruitfly, dragonfly, mouse, rat human. Specifically, enables instant...
Abstract The mammalian cortex is a laminar structure composed of many cell types densely interconnected in complex ways. Recent systematic efforts to map the mouse mesoscale connectome provide comprehensive projection data on interareal connections, but not at level specific classes or layers within cortical areas. We present here significant expansion Allen Mouse Brain Connectivity Atlas, with ∼1,000 new axonal mapping experiments across nearly all isocortical areas 49 Cre driver lines....
Fluorescent labeling allows for imaging and tracking of vesicles down to single-particle level. Among several options introduce fluorescence, staining lipid membranes with lipophilic dyes provides a straightforward approach without interfering vesicle content. However, incorporating molecules into in an aqueous solution is generally not efficient because their low water solubility. Here, we describe simple, fast (<30 min), highly effective procedure fluorescent including natural...
Abstract The cell-derived vesicles (CDVs) obtained using a proprietary extrusion process are the foundation of BioDrone platform technology. With superior productivity and versatility, this technology has garnered increasing attention in broad applications, particularly as drug delivery vehicle. Previously, we showed that CDVs exhibited varying levels expression for tetraspanin organelle membrane markers while revealing no discernible differences physical characteristics compared to...
Harnessing the natural ability of viruses to infect post-mitotic cells such as neurons has provided an explosion new methods manipulate and reconstruct neural circuits in vivo. Here we describe use recombinant adeno-associated viral vectors (rAAV) for axonal tract tracing nontransgenic transgenic Cre driver mice. Two protocols are presented stereotactic-guided placement rAAV into live mouse brain using iontophoretic or nanoliter pressure injections. The discussed here will result expression...
ABSTRACT As an anterograde neuronal tracer, recombinant adeno‐associated virus (AAV) has distinct advantages over the widely used biotinylated dextran amine (BDA). However, sensitivity and selectivity of AAV remain uncharacterized for many brain regions species. To validate this tracing method further, (serotype 1) was systematically compared with BDA as tracer by injecting both tracers into three cortical 15 subcortical in C57BL/6J mice. Identical parameters were our sequential...
Abstract Extracellular vesicles (EVs) released by a variety of cell types have been shown to act as natural delivery system for bioactive molecules such RNAs and proteins. EV therapy holds great promise safe cell‐free many immunological degenerative diseases. However, translation clinical application is limited several factors, including insufficient large‐scale manufacturing technologies low yield. We developed novel drug platform technology, BioDrone™, based on cell‐derived (CDVs) produced...
Abstract Membrane‐bound vesicles such as extracellular (EVs) can function biochemical effectors on target cells. Docking of the onto recipient plasma membranes depends their interaction with cell‐surface proteins, but a generalizable technique that quantitatively observe these vesicle–protein interactions (VPIs) is lacking. Here, we describe fluorescence microscopy measures VPIs between single and either in surface‐tethered or membrane‐embedded state. By employing cell‐derived (CDVs)...
ABSTRACT The connections between the claustrum and cortex in mouse are systematically investigated with adeno‐associated virus (AAV), an anterograde viral tracer. We first define boundary three‐dimensional structure of based on a variety molecular anatomical data. From AAV injections into 42 neocortical allocortical areas, we conclude that most cortical areas send bilateral projections to claustrum, majority being denser ipsilateral side. This includes prelimbic, infralimbic, medial,...
The posterior parietal cortex (PPC) is a major multimodal association implicated in variety of higher order cognitive functions, such as visuospatial perception, spatial attention, categorization, and decision-making. PPC known to receive inputs from collection sensory cortices well various subcortical areas integrate those facilitate the execution functions that require diverse information. Although many recent works have been performed with mouse model system, comprehensive understanding...