Paul Wohnoutka
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Advanced Neuroimaging Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Photoreceptor and optogenetics research
- Microtubule and mitosis dynamics
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Functional Brain Connectivity Studies
- Congenital heart defects research
Allen Institute
2006-2018
Allen Institute for Brain Science
2006-2018
Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The tumor's molecular and cellular landscapes are complex, their relationships to histologic features routinely used for diagnosis unclear. We present the Ivy Atlas, anatomically based transcriptional atlas of human glioblastoma aligns individual with genomic alterations gene expression patterns, thus assigning information most important morphologic hallmarks tumor. its clinical database freely accessible online data...
ABSTRACT Detailed anatomical understanding of the human brain is essential for unraveling its functional architecture, yet current reference atlases have major limitations such as lack whole‐brain coverage, relatively low image resolution, and sparse structural annotation. We present first digital atlas to incorporate neuroimaging, high‐resolution histology, chemoarchitecture across a complete adult female brain, consisting magnetic resonance imaging (MRI), diffusion‐weighted (DWI), 1,356...
ABSTRACT Detailed anatomical understanding of the human brain is essential for unraveling its functional architecture, yet current reference atlases have major limitations such as lack whole‐brain coverage, relatively low image resolution, and sparse structural annotation. We present first digital atlas to incorporate neuroimaging, high‐resolution histology, chemoarchitecture across a complete adult female brain, consisting magnetic resonance imaging (MRI), diffusion‐weighted (DWI), 1,356...
The Allen Brain Atlas (ABA) is a genomic scale, web-based 3D atlas of expression more than 20,000 genes in the mouse brain visualized using colorimetric situ hybridization. Completing ABA required imaging 1 million individual sections at cellular resolution. To facilitate these needs, high throughput, fully scalable, automated microscopy platform capable 3000 daily was developed. This system runs 24 h per day and 7 days week with minimal operator supervision. comprises barcode scanner,...