Phillip Bohn
A5011591095- Neural dynamics and brain function
- Functional Brain Connectivity Studies
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Neuroimaging Techniques and Applications
- Dementia and Cognitive Impairment Research
- Memory and Neural Mechanisms
- Cell Image Analysis Techniques
- Olfactory and Sensory Function Studies
- Microtubule and mitosis dynamics
- Single-cell and spatial transcriptomics
Allen Institute
2014-2022
Allen Institute for Brain Science
2014-2022
Significant advances in circuit-level analyses of the brain require tools that allow for labeling, modulation gene expression, and monitoring manipulation cellular activity specific cell types and/or anatomical regions. Large-scale projects individual laboratories have produced hundreds gene-specific promoter-driven Cre mouse lines invaluable enabling genetic access to subpopulations cells brain. However, potential utility each line may not be fully realized without systematic whole...
We present a unique, extensive, and open synaptic physiology analysis platform dataset. Through its application, we reveal principles that relate cell type to properties intralaminar circuit organization in the mouse human cortex. The dynamics of excitatory synapses align with postsynaptic subclass, whereas inhibitory synapse partly presynaptic subclass but considerable overlap. Synaptic are heterogeneous most subclass-to-subclass connections. two main axes heterogeneity strength...
The evolutionarily conserved default mode network (DMN) is a distributed set of brain regions coactivated during resting states that vulnerable to disorders. How disease affects the DMN unknown, but detailed anatomical descriptions could provide clues. Mice offer an opportunity investigate structural connectivity across spatial scales with cell-type resolution. We co-registered maps from functional magnetic resonance imaging and axonal tracing experiments into 3D Allen mouse reference atlas....
Abstract A variety of Alzheimer's disease (AD) mouse models overexpress mutant forms human amyloid precursor protein (APP), producing high levels β (Aβ) and forming plaques. However, the degree to which these mimic spatiotemporal patterns Aβ deposition in brains AD patients is unknown. Here, we mapped spatial distribution plaques across age three APP‐overexpression lines (APP/PS1, Tg2576, hAPP‐J20) using vivo labeling with methoxy‐X04, throughput whole brain imaging, an automated informatics...
Abstract The mammalian cortex is a laminar structure composed of many cell types densely interconnected in complex ways. Recent systematic efforts to map the mouse mesoscale connectome provide comprehensive projection data on interareal connections, but not at level specific classes or layers within cortical areas. We present here significant expansion Allen Mouse Brain Connectivity Atlas, with ∼1,000 new axonal mapping experiments across nearly all isocortical areas 49 Cre driver lines....
Subicular regions play important roles in spatial processing and many cognitive functions, these are mainly attributed to the subiculum (Sub) rather than prosubiculum (PS). Using single-cell RNA sequencing, we identify 27 transcriptomic cell types residing sub-domains of Sub PS. Based on situ expression reliable markers, precise boundaries PS consistently defined along dorsoventral axis. borders evaluate Cre-line specificity tracer injections, find bona fide projections topographically...
Abstract To elucidate cortical microcircuit structure and synaptic properties we present a unique, extensive, public physiology dataset analysis platform. Through its application, reveal principles that relate cell type to synapse intralaminar circuit organization in the mouse human cortex. The dynamics of excitatory synapses align with postsynaptic subclass, whereas inhibitory partly presynaptic subclass but considerable overlap. Despite these associations, are heterogeneous most...
Abstract A variety of Alzheimer’s disease (AD) mouse models overexpress mutant forms human amyloid precursor protein (APP), producing high levels β (Aβ) and forming plaques However, the degree to which these mimic spatiotemporal patterns Aβ deposition in brains AD patients is unknown. Here, we mapped spatial distribution plaques across ages three APP-overexpression lines (APP/PS1, Tg2576, hAPP-J20) using vivo labeling with methoxy-X04, throughput whole brain imaging, an automated...
Abstract The evolutionarily conserved default mode network (DMN) is characterized by temporally correlated activity between brain regions during resting states. DMN has emerged as a selectively vulnerable in multiple disorders, so understanding its anatomical composition will provide fundamental insight into how function impacted disease. Reproducible rodent analogs of the human offer an opportunity to investigate underlying and structural connectivity (SC) with high spatial cell type...
Summary Subicular region plays important roles in spatial processing and many cognitive functions these were mainly attributed to subiculum (Sub) rather than prosubiculum (PS). Using single-cell RNA-sequencing (scRNA-seq) technique we have identified up 27 distinct transcriptomic clusters/cell types, which registered anatomical sub-domains Sub PS. Based on reliable molecular markers derived from clustering situ hybridization data, the precise boundaries of PS been consistently defined along...
Amyloid beta (Aβ) plaques are not distributed uniformly across the brain, but instead form in specific structures, then spread a predictable pattern through selectively vulnerable brain networks. A variety of Alzheimer's disease (AD) mouse models overexpress mutant forms human amyloid precursor protein (hAPP) to produce high levels Aβ. These used examine mechanisms underlying plaque accumulation, degree which they mimic spatiotemporal patterns Aβ deposition patients remains an open question....