A5018876789- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Malaria Research and Control
- Neuroscience and Neural Engineering
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Neuroinflammation and Neurodegeneration Mechanisms
- Evolution and Genetic Dynamics
- Viral Infections and Outbreaks Research
- Vector-borne infectious diseases
- Memory and Neural Mechanisms
- Viral Infections and Immunology Research
- Advanced Fluorescence Microscopy Techniques
- Virus-based gene therapy research
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Value Engineering and Management
- Bioinformatics and Genomic Networks
- Pluripotent Stem Cells Research
- Congenital heart defects research
- HIV Research and Treatment
Allen Institute
2016-2025
Allen Institute for Brain Science
2016-2025
New York State Department of Health
2003-2023
Wadsworth Center
2003-2023
University at Albany, State University of New York
2021-2022
Public Health Agency of Canada
2022
University of America
2022
Catholic University of America
2022
University of Massachusetts Amherst
2021
Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The tumor's molecular and cellular landscapes are complex, their relationships to histologic features routinely used for diagnosis unclear. We present the Ivy Atlas, anatomically based transcriptional atlas of human glioblastoma aligns individual with genomic alterations gene expression patterns, thus assigning information most important morphologic hallmarks tumor. its clinical database freely accessible online data...
We present a unique, extensive, and open synaptic physiology analysis platform dataset. Through its application, we reveal principles that relate cell type to properties intralaminar circuit organization in the mouse human cortex. The dynamics of excitatory synapses align with postsynaptic subclass, whereas inhibitory synapse partly presynaptic subclass but considerable overlap. Synaptic are heterogeneous most subclass-to-subclass connections. two main axes heterogeneity strength...
Abstract The neocortex is disproportionately expanded in human compared with mouse 1,2 , both its total volume relative to subcortical structures and the proportion occupied by supragranular layers composed of neurons that selectively make connections within other telencephalic structures. Single-cell transcriptomic analyses show an increased diversity glutamatergic neuron types pronounced gradients as a function cortical depth 3 . Here, probe functional anatomical correlates this diversity,...
Abstract West Nile (WN) virus was found throughout New York State in 2000, with the epicenter City and surrounding counties. We tested 3,403 dead birds 9,954 mosquito pools for WN during transmission season. Sixty-three avian species, representing 30 families 14 orders, positive virus. The highest proportion of that American Crows (67% positive, n=907). Eight four genera, were minimum infection rate per 1,000 mosquitoes (MIR) Culex pipiens epicenter: 3.53 entire season 7.49 peak week August...
ABSTRACT The recent outbreaks of West Nile virus (WNV) in the northeastern United States and other regions world have made it essential to develop an efficient protocol for surveillance WNV. In present report, we describe a high-throughput procedure that combines automated RNA extraction, amplification, detection WNV RNA. analyzed 96 samples approximately 4.5 h. A robotic system, ABI Prism 6700 Automated Nucleic Acid workstation, extracted set up reactions real-time reverse transcription...
A polymerase chain reaction (PCR) protocol was developed to identify host bloodmeals from mosquitoes. Primers for the cytochrome b gene were designed distinguish between mammalian and avian further differentiate among four orders: passeriformes, falconiformes, columbiformes, galliformes. The assay validated by testing tissues 18 species of three two American crows distinguished other passeriformes restriction enzyme digestion. Host engorged mosquitoes collected in New York State identified...
The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution each cell type processes guided by essential for understanding its function in health and disease. We used transcriptomic epigenomic cortical taxonomies from mouse human define marker genes putative enhancers created a large toolkit transgenic lines enhancer AAVs selective targeting populations. report evaluation fifteen new driver lines, two reporter >800 different...
Abstract West Nile (WN) virus was found throughout New York State in 2000, with the epicenter City and surrounding counties. We tested 3,403 dead birds 9,954 mosquito pools for WN during transmission season. Sixty-three avian species, representing 30 families 14 orders, positive virus. The highest proportion of that American Crows (67% positive, n=907). Eight four genera, were minimum infection rate per 1,000 mosquitoes (MIR) Culex pipiens epicenter: 3.53 entire season 7.49 peak week August...
Abstract The mammalian visual system, from retina to neocortex, has been extensively studied at both anatomical and functional levels. Anatomy indicates the cortico-thalamic system is hierarchical, but characterization of cellular-level interactions across multiple levels this hierarchy lacking, partially due challenge simultaneously recording activity numerous regions. Here, we describe a large, open dataset (part Allen Brain Observatory ) that surveys spiking units in six cortical two...
Subicular regions play important roles in spatial processing and many cognitive functions, these are mainly attributed to the subiculum (Sub) rather than prosubiculum (PS). Using single-cell RNA sequencing, we identify 27 transcriptomic cell types residing sub-domains of Sub PS. Based on situ expression reliable markers, precise boundaries PS consistently defined along dorsoventral axis. borders evaluate Cre-line specificity tracer injections, find bona fide projections topographically...
Extracellular electrophysiology and two-photon calcium imaging are widely used methods for measuring physiological activity with single-cell resolution across large populations of cortical neurons. While each these two modalities has distinct advantages disadvantages, neither provides complete, unbiased information about the underlying neural population. Here, we compare evoked responses in visual cortex recorded awake mice under highly standardized conditions using either genetically...
We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during 2000 transmission season to determine whether they differed genetically each other and initial strain isolated 1999. The complete genes these were amplified by reverse transcription-polymerase chain reaction. resulting aligned, genetic distances computed, a phylogenetic tree was constructed. Ten (0.7%) 1,503 positions polymorphic one or more sequences. 0.003 less. WN...
West Nile virus (WNV) has successfully spread throughout the USA, Canada, Mexico, Caribbean and parts of Central South America since its 1999 introduction into North America. Despite infecting a broad range both mosquito avian species, remains highly genetically conserved. This lack evolutionary change over space time is common with many arboviruses frequently attributed to adaptive constraints resulting from cycling between vertebrate hosts invertebrate vectors. WNV, like most RNA viruses...
Abstract Neurons are frequently classified into distinct groups or cell types on the basis of structural, physiological, genetic attributes. To better constrain definition neuronal types, we characterized transcriptomes and intrinsic physiological properties over 3,700 GABAergic mouse visual cortical neurons reconstructed local morphologies 350 those neurons. We found that most transcriptomic (t-types) occupy specific laminar positions within cortex, many t-types exhibit consistent...
The neocortex is disproportionately expanded in human compared to mouse, both its total volume relative subcortical structures and the proportion occupied by supragranular layers that selectively make connections within cortex other telencephalic structures. Single-cell transcriptomic analyses of mouse show an increased diversity glutamatergic neuron types pronounced gradients as a function cortical depth. To probe functional anatomical correlates this diversity, we describe robust Patch-seq...
Abstract We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during 2000 transmission season to determine whether they differed genetically each other and initial strain isolated 1999. The complete genes these were amplified by reverse transcription-polymerase chain reaction. resulting aligned, genetic distances computed, a phylogenetic tree was constructed. Ten (0.7%) 1,503 positions polymorphic one or more sequences. 0.003...
ABSTRACT The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions world have made it essential to develop efficient, sensitive, rapid protocols for surveillance. Laboratory testing is backbone any surveillance program. Protocols detect presence WNV been refined since 1999 sensitivity, speed, efficiency, specificity. This paper presents currently used by New York State Department Health handle vertebrate mosquito specimens that submitted...
During 2018, Heartland virus RNA was detected in an Amblyomma americanum tick removed from a resident of Suffolk County, New York, USA. The person showed seroconversion. Tick surveillance and white-tailed deer (Odocoileus virginianus) serosurveys widespread distribution emphasizing need for disease anywhere A. ticks are established or emerging.
There is a common assumption that viral lysis buffers are sufficient to render viruses noninfectious. This has significant impact on the way biological samples processed, labeled, and handled for shipment. Several buffers, including TRIzol, AVL, RLT, MagMAX, easyMAG, were examined their capacity inactivate representative from multiple genera, alphavirus, bunyavirus, flavivirus, adenovirus, enterovirus, influenza B, simplexvirus. Viruses noninfectious following treatment with easyMAG while...
We present an enhancer-AAV toolbox for accessing and perturbing striatal cell types circuits. Best-in-class vectors were curated major neuron populations including medium spiny neurons (MSNs), direct- indirect-pathway MSNs, Sst-Chodl, Pvalb-Pthlh, cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes virus delivery, with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable...