Charles Cobbs
A5019173998- Cytomegalovirus and herpesvirus research
- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Neural dynamics and brain function
- Brain Metastases and Treatment
- interferon and immune responses
- Herpesvirus Infections and Treatments
- Neuroscience and Neuropharmacology Research
- Cancer Research and Treatments
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Neuroscience and Neural Engineering
- Cancer-related molecular mechanisms research
- Lung Cancer Research Studies
- RNA regulation and disease
- Toxoplasma gondii Research Studies
- Viral-associated cancers and disorders
- Cancer, Hypoxia, and Metabolism
- Mycobacterium research and diagnosis
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
Swedish Medical Center
2015-2024
Neuroscience Institute
2015-2024
California Pacific Medical Center
2009-2023
Neurological Surgery
1996-2023
University of California, San Francisco
1999-2023
Seattle University
2014-2022
Swedish Medical Center
2018-2022
Ivy Foundation
2013-2021
Emory University
2018
GTx (United States)
2018
Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The tumor's molecular and cellular landscapes are complex, their relationships to histologic features routinely used for diagnosis unclear. We present the Ivy Atlas, anatomically based transcriptional atlas of human glioblastoma aligns individual with genomic alterations gene expression patterns, thus assigning information most important morphologic hallmarks tumor. its clinical database freely accessible online data...
Standard therapy for glioblastoma includes surgery, radiotherapy, and temozolomide. This Phase 3 trial evaluates the addition of an autologous tumor lysate-pulsed dendritic cell vaccine (DCVax®-L) to standard newly diagnosed glioblastoma.After surgery chemoradiotherapy, patients were randomized (2:1) receive temozolomide plus DCVax-L (n = 232) or placebo 99). Following recurrence, all allowed DCVax-L, without unblinding. The primary endpoint was progression free survival (PFS); secondary...
The human cytomegalovirus (HCMV) and glioma symposium was convened on April 17, 2011 in Washington, DC, attended by oncologists virologists involved studying the relationship between HCMV gliomas. purpose of meeting to reach a consensus role pathology gliomas clarify directions for future research. First, group summarized data that describe how biology overlaps with key pathways cancer. Then, basis published ongoing research, reached there is sufficient evidence conclude sequences viral gene...
This protocol is a practical guide to the N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. Numerous recent studies have validated utility this for enhancing neuronal preservation and overall viability. The implementation technique by early adopters has facilitated detailed investigations into function using diverse experimental applications spanning wide range animal ages, regions, cell types. Steps are outlined carrying out an optimized NMDG artificial...
Glioblastoma is the most lethal primary brain cancer. Clinical outcomes for glioblastoma remain poor, and new treatments are needed.To investigate whether adding autologous tumor lysate-loaded dendritic cell vaccine (DCVax-L) to standard of care (SOC) extends survival among patients with glioblastoma.This phase 3, prospective, externally controlled nonrandomized trial compared overall (OS) in newly diagnosed (nGBM) recurrent (rGBM) treated DCVax-L plus SOC vs contemporaneous matched external...
Generating a comprehensive description of cortical networks requires large-scale, systematic approach. To that end, we have begun pipeline project using multipatch electrophysiology, supplemented with two-photon optogenetics, to characterize connectivity and synaptic signaling between classes neurons in adult mouse primary visual cortex (V1) human cortex. We focus on producing results detailed enough for the generation computational models enabling comparison future studies. Here, report our...
Viral genetic tools that target specific brain cell types could transform basic neuroscience and targeted gene therapy. Here, we use comparative open chromatin analysis to identify thousands of human-neocortical-subclass-specific putative enhancers from across the genome control expression in adeno-associated virus (AAV) vectors. The cellular specificity reporter enhancer-AAVs is established by molecular profiling after systemic AAV delivery mouse. Over 30% produce subclass, including both...
Human cortex transcriptomic studies have revealed a hierarchical organization of γ-aminobutyric acid-producing (GABAergic) neurons from subclasses to high diversity more granular types. Rapid GABAergic neuron viral genetic labeling plus Patch-seq (patch-clamp electrophysiology single-cell RNA sequencing) sampling in human brain slices was used reliably target and analyze individual This characterization elucidated transitions between PVALB SST subclasses, morphological heterogeneity within...
Neocortical layer 1 (L1) is a site of convergence between pyramidal-neuron dendrites and feedback axons where local inhibitory signaling can profoundly shape cortical processing. Evolutionary expansion human neocortex marked by distinctive pyramidal neurons with extensive L1 branching, but whether interneurons are similarly diverse underexplored. Using Patch-seq recordings from neurosurgical tissue, we identified four transcriptomic subclasses mouse homologs, along distinct subtypes types...
No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Sep 2003High Prevalence Human Cytomegalovirus in Prostatic Intraepithelial Neoplasia and Carcinoma MINU SAMANTA, LUALHATI HARKINS, KATRIN KLEMM, WILLIAM J. BRITT, CHARLES S. COBBS SAMANTAMINU SAMANTA , HARKINSLUALHATI HARKINS KLEMMKATRIN KLEMM BRITTWILLIAM BRITT COBBSCHARLES View All Author Informationhttps://doi.org/10.1097/01.ju.0000080263.46164.97AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints...
Human cytomegalovirus (HCMV) establishes a persistent life-long infection, and can cause severe pathology in the fetus immunocompromised host1. Breast milk is primary route of transmission humans worldwide, breast epithelium thus likely site infection and/or reactivation, though this phenomenon has not previously been demonstrated. Increasing evidence indicates HCMV modulate signaling pathways associated with oncogenesis. We hypothesized that occurs normal adult viral expression might be...
Abstract Human cytomegalovirus (HCMV) infections are seen often in glioblastoma multiforme (GBM) tumors, but whether the virus contributes to GBM pathogenesis is unclear. In this study, we explored an oncogenic role for G-protein–coupled receptor-like protein US28 encoded by HCMV that found be expressed widely human GBMs. Immunohistochemical and reverse transcriptase PCR approaches established was approximately 60% of tissues primary cultures examined. either uninfected cells or neural...
One third of humans are infected lifelong with the brain-dwelling, protozoan parasite, Toxoplasma gondii. Approximately fifteen million these have congenital toxoplasmosis. Although neurobehavioral disease is associated seropositivity, causality unproven. To better understand what this parasite does to human brains, we performed a comprehensive systems analysis brain: We identified susceptibility genes for toxoplasmosis in our cohort and found expressed brain. Transcriptomic quantitative...