A5053087197- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Advanced Fluorescence Microscopy Techniques
- Memory and Neural Mechanisms
- Gene expression and cancer classification
- Lipid Membrane Structure and Behavior
- Cell Image Analysis Techniques
- Advanced Memory and Neural Computing
- Receptor Mechanisms and Signaling
- Nuclear Physics and Applications
- Glioma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Pancreatic function and diabetes
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Regulation of Appetite and Obesity
University of Szeged
2012-2023
Allen Institute
2023
Allen Institute for Neural Dynamics
2023
Janelia Research Campus
2021-2023
Howard Hughes Medical Institute
2021-2023
Hungarian Academy of Sciences
2012-2021
University of North Carolina Health Care
2021
University of North Carolina at Chapel Hill
2021
Simons Foundation
2021
North Carolina State University
2021
Neocortical layer 1 (L1) is a site of convergence between pyramidal-neuron dendrites and feedback axons where local inhibitory signaling can profoundly shape cortical processing. Evolutionary expansion human neocortex marked by distinctive pyramidal neurons with extensive L1 branching, but whether interneurons are similarly diverse underexplored. Using Patch-seq recordings from neurosurgical tissue, we identified four transcriptomic subclasses mouse homologs, along distinct subtypes types...
Concentrations of insulin in the brain are severalfold higher than blood plasma levels. Insulin regulates metabolism, molecular composition, and cognitive performance microcircuits reduces food intake; cerebral levels altered diabetes, aging, obesity, Alzheimer's disease. Released by pancreatic β cells, passes blood-brain barrier, but sources locally released still remain unclear. We find that is strongly expressed GABAergic neurogliaform cells cortex rat detected single-cell digital PCR....
Classic theories link cognitive abilities to synaptic properties and human-specific biophysical features of synapses might contribute the unparalleled performance human cerebral cortex. Paired recordings multiple probability fluctuation analysis revealed similar quantal sizes, but 4-times more functional release sites in pyramidal cell fast-spiking interneuron connections compared rats. These were mediated on average by three contacts both species. Each presynaptic active zone (AZ) contains...
Abstract Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. Here, we demonstrate tool that fully automatically performs electrophysiological recordings in label-free tissue slices. The automation covers the detection cells images, calibration micropipette movement, approach to cell with pipette, formation whole-cell configuration, recording. based on deep learning. model trained new image database unlabeled brain pipette tip approaching phase use analysis...
Selective serotonin reuptake inhibitors are the most widely prescribed drugs targeting CNS with acute and chronic effects in cognitive, emotional behavioral processes. This suggests that microcircuits of human cerebral cortex powerfully modulated by selective inhibitors, however, direct measurements serotonergic regulation on synaptic interactions missing. Using multiple whole-cell patch-clamp recordings from neurons cortical slices derived nonpathological samples prefrontal cortex, we show...
Neural population activity determines the timing of synaptic inputs, which arrive to dendrites, cell bodies, and axon initial segments (AISs) cortical neurons. Action potential initiation in AIS (AIS-APs) is driven by input integration, phase preference AIS-APs during network oscillations characteristic classes. Distal regions axons do not receive yet experimental induction protocols can trigger retroaxonal action potentials (RA-APs) distal from soma. We report spontaneously occurring RA-APs...
Neuroscience can only be reproducible when its key methods are quantitative and interpretable. Calcium imaging is such a method which, however, records neuronal activity indirectly therefore difficult to interpret. These difficulties arise primarily from the kinetics, nonlinearity, sensitivity of calcium indicator, but also depend on for signal analysis. Here, we evaluate ability recently developed indicator GCaMP8 reveal spiking, investigate how existing spike inference (CASCADE, OASIS,...
Whole-cell patch-clamp recording enables detection of electrophysiological signals from single neurons as well harvesting perisomatic RNA through the patch pipette for subsequent gene expression analysis. Amplification and profiling with traditional quantitative real-time PCR (qRT-PCR) do not provide exact quantitation due to experimental variation caused by limited amount nucleic acid in a cell. Here we describe protocol quantifying mRNA or miRNA individual after using high-density...
Abstract We describe convergent evidence from transcriptomics, morphology and physiology for a specialized GABAergic neuron subtype in human cortex. Using unbiased single nucleus RNA sequencing, we identify ten interneuron subtypes with combinatorial gene signatures cortical layer 1 characterize novel group of interneurons anatomical features never described rodents having large, “rosehip”-like axonal boutons compact arborization. These rosehip cells show an immunohistochemical profile...
Functional and molecular changes associated with pathophysiological conditions are relatively easily detected based on tissue samples collected from patients. Population specific cellular responses to disease might remain undiscovered in taken organs formed by a multitude of cell types. This is particularly apparent the human cerebral cortex composed yet undefined number neuron types potentially different involvement processes. We combined electrophysiology, anatomy single digital PCR...
Abstract Neural representations of information are shaped by local network interactions. Previous studies linking neural coding and cortical connectivity focused on stimulus selectivity in the sensory cortex 1–4 . Here we study activity motor during naturalistic behavior which mice gathered rewards with multidirectional tongue reaching. This does not require training thus allowed us to probe before its is learning a specific task. Neurons typically responded after reaching movements...
ABSTRACT Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. We have developed tool that fully automatically performs electrophysiological recordings in label-free tissue slices. The automation covers the detection cells images, calibration micropipette movement, approach to cell with pipette, formation whole-cell configuration, recording. based on deep learning. model was trained new image database unlabeled brain pipette tip approaching phase use analysis...
Abstract Optical microscopy methods such as calcium and voltage imaging already enable fast activity readout (30-1000Hz) of large neuronal populations using light. However, the lack corresponding advances in online algorithms has slowed progress retrieving information about neural during or shortly after an experiment. This technological gap not only prevents execution novel real-time closed-loop experiments, but also hampers experiment-analysis-theory turnover for high-throughput...
Abstract Neural population activity determines the timing of synaptic inputs, which arrive to dendrites, cell bodies and axon initial segments (AISs) cortical neurons. Action potential initiation in AIS (AIS-APs) is driven by input integration, phase preference AIS-APs during network oscillations characteristic classes. Distal regions axons do not receive yet experimental induction protocols can trigger retroaxonal action potentials (RA-APs) distal from soma. We report spontaneously...
Protocol for Head Post and Cranial Window Surgery developed at Janelia Research Campus in the Svoboda Lab Developed by Karel Trachtenberg et al 2002 Improved Anthony Holtmaat 2006 2009 Daniel Huber 2012 Compiled Bryan Maclennan (2013), Abridged version Courtney Davis (2015) to protocols.io JJ Kim (2019) Note: This outline only provides practical step-by-step advice. All surgeons should be familiar with carefully adhere official animal protocol current procedures posted on lab wiki....