Adam Amster
A5038828244- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Ancient Near East History
- Ancient Egypt and Archaeology
Allen Institute
2023-2025
Allen Institute for Brain Science
2025
The detection of novel stimuli is critical to learn and survive in a dynamic environment. Though powerfully affect brain activity, their impact on specific cell types circuits not well understood. Disinhibition one candidate mechanism for novelty-induced enhancements activity. Here we characterize the stimulus novelty disinhibitory circuit components using longitudinal 2-photon calcium imaging Vip, Sst, excitatory populations mouse visual cortex. Mice behavioral task with that become highly...
We present an enhancer-AAV toolbox for accessing and perturbing striatal cell types circuits. Best-in-class vectors were curated major neuron populations including medium spiny neurons (MSNs), direct- indirect-pathway MSNs, Sst-Chodl, Pvalb-Pthlh, cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes virus delivery, with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable...
The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution each cell type processes guided by essential for understanding its function in health and disease. We use transcriptomic epigenomic cortical cell-type taxonomies from mouse human define marker genes putative enhancers create a large toolkit transgenic lines enhancer adeno-associated viruses (AAVs) selective targeting populations. report creation evaluation fifteen driver...
We present an enhancer AAV toolbox for accessing and perturbing striatal cell types circuits. Best-in-class vectors were curated major neuron populations including medium spiny neurons (MSNs), direct indirect pathway MSNs, as well Sst-Chodl, Pvalb-Pthlh, cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes virus delivery, with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable...
In 1949, Donald Hebb proposed that groups of neurons activate stereotypically form the organizational building blocks perception, cognition, and behavior. He theorized repeated activations induce structural changes needed to group in these assemblies. Despite Hebb's enduring influence, testing his predictions at relevant scales has been technically challenging. Here, we test theory using a novel, large-scale dataset featuring vivo calcium fluorescence imaging neural activity with postmortem...