A5024393630- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- Intracranial Aneurysms: Treatment and Complications
- Vascular Malformations Diagnosis and Treatment
- Moyamoya disease diagnosis and treatment
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Liquid Crystal Research Advancements
- Gene Regulatory Network Analysis
- Tendon Structure and Treatment
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neural Engineering
- Sugarcane Cultivation and Processing
- Intracerebral and Subarachnoid Hemorrhage Research
- Vascular Anomalies and Treatments
- Cardiac Structural Anomalies and Repair
- Polymer Surface Interaction Studies
- Extracellular vesicles in disease
- Microscopic Colitis
- Axon Guidance and Neuronal Signaling
Sunshine Coast University Hospital
2024
Merck (Germany)
2022-2024
Allen Institute
2017-2023
Allen Institute for Brain Science
2017-2023
University of California, San Francisco
2013-2023
Westchester Medical Center
2021-2022
Merck (Japan)
2022
Merck (United Kingdom)
2019
John Brown University
2019
Cardinal Tien Hospital
2018
Fluorescent calcium indicators are often used to investigate neural dynamics, but the relationship between fluorescence and action potentials (APs) remains unclear. Most APs can be detected when soma almost fills microscope's field of view, image populations neurons, necessitating a large generating fewer photons per neuron, compromising AP detection. Here, we characterized AP-fluorescence transfer function in vivo for 48 layer 2/3 pyramidal neurons primary visual cortex, with simultaneous...
Summary To understand how the brain processes sensory information to guide behavior, we must know stimulus representations are transformed throughout visual cortex. Here report an open, large-scale physiological survey of neural activity in awake mouse cortex: Allen Brain Observatory Visual Coding dataset. This publicly available dataset includes cortical from nearly 60,000 neurons collected 6 areas, 4 layers, and 12 transgenic lines 221 adult mice, response a systematic set stimuli. Using...
Significance Brain arteriovenous malformations are focal lesions of enlarged, tangled vessels that shunt blood from arteries directly to veins. They can cause ischemia, hemorrhage, disability, and death, particularly in young people, accounting for 50% childhood stroke. The molecular etiology the disease remains poorly understood, hindering development therapeutic treatments. Here, we report that, an animal model, lesion arises enlargement capillary-like vessels. Notch signaling endothelium...
Abstract A fundamental question in hematopoietic development is how multipotent progenitors achieve precise identities, while the themselves maintain quiescence. In Drosophila melanogaster larvae, support production of three lineages, exhibit quiescence response to cues from a niche, and their differentiated progeny. Infection by parasitic wasps alters course hematopoiesis. Here we address role Notch (N) signaling lamellocyte differentiation wasp infection. We show that activity moderately...
Local sol-gel transitions of the cytoskeleton modulate cell shape changes, which are required for essential cellular functions, including motility and adhesion. In vitro studies using purified cytoskeletal proteins have suggested molecular mechanisms regulation mechanics; however, mechanical behavior living cells signaling pathways by it is regulated remains largely unknown. To address this issue, we used a nanoscale sensing method, intracellular microrheology, to examine response activation...
Abstract Spatiotemporally synchronised neuronal activity is central to sensation, motion and cognition. Brain circuits consist of dynamically interconnected cell-types, thus elucidating how neuron types synergise within the network key understand orchestra. Here we show that in neocortex neuron-network coupling cell-subtype specific. Employing vivo two-photon (2-p) Calcium (Ca) imaging 2-p targeted whole-cell recordings, cell-type specifically investigated profiles genetically defined...
For chemotherapy patients, intestinal mucositis is a frequent complication. Previously, we evaluated the beneficial effect of oral probiotics in 5-Fluorouracil (5-FU) induced BALB/c mice. Here, used SCID/NOD mice instead to simulate immunodeficiency patients: first, evaluate safety probiotic supplementation and second, determine response 5-FU mucositis.Thirty-six were injected with saline (three control groups) or experimental intraperitoneally daily for five days. Mice given either daily,...
Acute lung injury (ALI) is a life-threatening condition with limited treatment options. The pathogenesis of ALI involves macrophage-mediated disruption and subsequent repair the alveolar barriers, which ultimately results in damage regeneration, highlighting pivotal role macrophage polarization ALI. Although exosomes derived from mesenchymal stromal cells have been established as influential modulators polarization, specific exosomal microRNAs (miRNAs) remains underexplored. This study aimed...
We report infection with the phytopathogen Pantoea stewartii subspecies indologenes in a macadamia farmer from southeast Queensland, Australia. The patient had bloodstream and pneumonia develop after an unidentified inoculation event. Investigation determined that most likely mode of transmission was inhalation environmental source on farm.
The functions of blood flow in the morphogenesis mammalian arteries and veins are not well understood. We examined development dorsal aorta (DA) cardinal vein (CV) Ncx1 -/- mutants, which lack due to a deficiency sodium calcium ion exchanger expressed specifically heart. mutant DA CV were abnormally connected. endothelium lacked normal expression arterial markers ephrin-B2 Connexin-40. Notch1 activation, known promote specification, was decreased endothelial cells (ECs), ectopically venous...
Abstract Multiphoton calcium imaging is commonly used to monitor the spiking of large populations neurons. Recovering action potentials from fluorescence necessitates calibration experiments, often with simultaneous and cell-attached recording. Here we performed for conditions matching those Allen Brain Observatory. We developed a novel crowd-sourced, algorithmic approach quality control. Our final data set was 50 recordings 35 neurons in 3 mouse lines. indicated that or more spikes were...
Mechanisms underlying arteriovenous malformations (AVMs) are poorly understood. Using mice with endothelial cell (EC) expression of constitutively active Notch4 (Notch4* EC ), we show decreased arteriolar tone in vivo during brain AVM initiation. Reduced vascular is a primary effect Notch4* , as isolated pial arteries from asymptomatic exhibited reduced pressure-induced arterial ex vivo. The nitric oxide (NO) synthase (NOS) inhibitor NG-nitro- l -arginine (L-NNA) corrected defects both...
Abstract Two-photon calcium imaging is often used with genetically encoded indicators (GECIs) to investigate neural dynamics, but the relationship between fluorescence and action potentials (spikes) remains unclear. Pioneering work linked electrophysiology in vivo viral GECI expression, albeit a small number of cells. Here we characterized spikefluorescence transfer function 91 layer 2/3 pyramidal neurons primary visual cortex four transgenic mouse lines expressing GCaMP6s or GCaMP6f. We...
SUMMARY Modern genetic approaches are powerful in providing access to diverse types of neurons within the mammalian brain and greatly facilitating study their function. We here report a large set driver reporter transgenic mouse lines, including 23 new lines targeting variety cortical subcortical cell populations 26 expressing an array molecular tools. In particular, we describe TIGRE2.0 platform introduce Cre-dependent that enable optical physiology, optogenetics, sparse labeling...
Mucormycosis is a devastating disease with high mortality rate, typically affecting immunosuppressed individuals. Postoperative surgical site infections due to mucromycosis are rare, only handful of cases reported in the literature. Here, we describe fatal case post operative abdominal wound infection caused by mucormycosis an immunocompetent man his 70 s, who developed following laparotomy for bowel perforation. Initially, growth fungal species from superficial swab was not considered...
Modern genetic approaches are powerful in providing access to diverse types of neurons within the mammalian brain and greatly facilitating study their function. We here report a large set driver reporter transgenic mouse lines, including 23 new lines targeting variety cortical subcortical cell populations 26 expressing an array molecular tools. In particular, we describe TIGRE2.0 platform introduce Cre-dependent that enable optical physiology, optogenetics, sparse labeling...