A5022193625- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Radioactive element chemistry and processing
- Oral microbiology and periodontitis research
- Radioactive contamination and transfer
- Fluoride Effects and Removal
- Semiconductor materials and devices
- Nuclear and radioactivity studies
- RNA Interference and Gene Delivery
- Salivary Gland Disorders and Functions
- RNA and protein synthesis mechanisms
- Mine drainage and remediation techniques
- Advancements in Semiconductor Devices and Circuit Design
- Cell death mechanisms and regulation
- Graphite, nuclear technology, radiation studies
- Virus-based gene therapy research
- Enzyme Structure and Function
- Heat shock proteins research
- X-ray Diffraction in Crystallography
- Legume Nitrogen Fixing Symbiosis
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Silicon and Solar Cell Technologies
- Thin-Film Transistor Technologies
- CRISPR and Genetic Engineering
Yokohama City University Medical Center
2009-2025
Yokohama City University
2009-2025
Hokkaido University
2011-2024
Geological Survey of Japan
2002-2024
National Institute of Advanced Industrial Science and Technology
2002-2024
Hosei University
2010-2023
Tokai University
1999-2022
Louis Pasteur Center for Medical Research
2021
Sapporo Medical University
2003-2016
Kanagawa Cancer Center
2014
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes organism, only 192 were shown be indispensable by this or previous work. Another 79 predicted essential. The vast majority essential categorized relatively few domains cell metabolism, with about half involved information processing, one-fifth synthesis envelope and determination shape division, one-tenth related...
Asymmetric division during sporulation by Bacillus subtilis generates a mother cell that undergoes 5-h program of differentiation. The is governed hierarchical cascade consisting the transcription factors: σE, σK, GerE, GerR, and SpoIIID. consists activation repression 383 genes. σE factor turns on 262 genes, including those for GerR These DNA-binding proteins downregulate almost half genes in regulon. In addition, SpoIIID ten involved appearance σK. Next, σK activates 75 additional GerE....
Article17 November 2015Open Access An experimentally supported model of the Bacillus subtilis global transcriptional regulatory network Mario L Arrieta-Ortiz Center for Genomics and Systems Biology, Department New York University, York, NY, USA Search more papers by this author Christoph Hafemeister Ashley Rose Bate Timothy Chu Alex Greenfield Bentley Shuster Samantha N Barry Matthew Gallitto Brian Liu Thadeous Kacmarczyk Francis Santoriello Jie Chen Christopher DA Rodrigues Microbiology...
ABSTRACT The Bacillus subtilis skin element confers resistance to arsenate and arsenite. ars operon in the contains four genes order arsR , ORF2 arsB arsC . Three of these are homologous from staphylococcal plasmid pI258, while no homologs have been found. Inactivation or results either constitutive expression an arsenite- arsenate-sensitive phenotype, respectively. These suggest that ArsR, ArsB, ArsC function as a negative regulator, membrane-associated protein need for extrusion arsenite,...
ABSTRACT NADH dehydrogenase is a key component of the respiratory chain. It catalyzes oxidation by transferring electrons to ubiquinone and establishes proton motive force across cell membrane. The yjlD (renamed ndh ) gene Bacillus subtilis predicted encode an enzyme similar II Escherichia coli , encoded gene. We have shown that yjlC-ndh operon negatively regulated YdiH Rex), homolog Rex in Streptomyces coelicolor redox-sensing transcriptional regulator responds NADH/NAD + ratio. regulates...
The purpose of this study was to determine the usefulness salivary biochemical markers for screening periodontal disease and examine agreement between results saliva enzyme tests those probing depth. present included a total 187 subjects who underwent annual medical check-ups at Comprehensive Health Care Center, Honjo, Saitama Prefecture, Japan. Periodontal pocket performed with WHO probe, various enzymes parameters in were measured. For lactate dehydrogenase (LDH), proportions five...
Temperate phages infect bacteria by injecting their DNA into bacterial cells, where it becomes incorporated the host genome as a prophage. In of Bacillus subtilis 168, an active prophage, SPβ, is inserted polysaccharide synthesis gene, spsM. Here, we show that rearrangement occurs during sporulation to reconstitute functional composite spsM gene precise excision SPβ from chromosome. requires putative site-specific recombinase, SprA, and accessory protein, SprB. A minimized all genes were...
Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients essential for general health. This study was performed to evaluate prospectively the diagnostic utility blood IgG antibody titer test against periodontal pathogens. Oral examination performed, titers pathogens were measured by ELISA in 1,387 individuals. The cut-off value determined receiver operating characteristic curve analysis,...
Transforming growth factor-β (TGF-β) is a major inducer of epithelial-mesenchymal transition (EMT) in different cell types. TGF-β-mediated EMT thought to contribute tumour spread and metastasis. Sialyl Lewis antigens synthesised by fucosyltransferase (FUT) 3 FUT6 are highly expressed patients with metastatic colorectal cancer (CRC) utilised as markers for detection evaluation treatment efficacy. However, the role FUT3 augmenting malignant potential CRC induced TGF-β unclear.Colorectal lines...
A promising technique to form the silicon-on-nothing structure is presented as an alternative silicon-on-insulator structure. large plate-shaped empty space in silicon (ESS) below surface of substrate can be fabricated by connecting spherical spaces, which are formed migration Si on patterned substrate. The ESS has potential change microprocess for fabrication large-scale integrated circuits and it applied various manufacturing technologies.
SUMMARY: As part of the Bacillus subtilis genome sequencing project, we have determined complete nucleotide sequence a skin element which is located between spoIVCB and spoIIIC. The entire this 48032 bp in length, contains 57 ORFs with putative ribosome-binding sites. Two them correspond to previously sequenced characterized genes, cwIA spoIVCA. Furthermore, seven ORF products identified show interesting similarities known proteins present data banks, including ø105 immunity repressor,...
Abstract Generation of reactive oxygen species (ROS) and activation caspase cascade are both indispensable in Fas-mediated apoptotic signaling. Although ROS was presumed to affect the activity on basis findings that antioxidants inhibited caspases stimulation by itself activated caspases, mechanism which these cellular events integrated Fas signaling is presently unclear. In this study, using human T cell leukemia Jurkat cells as well an vitro reconstitution system, we demonstrate required...
The nucleotide sequence of the sporulation gene spoIVC cisA in Bacillus subtilis was determined and found to encode a protein 500 amino acid residues with calculated molecular weight 57,481, which is good agreement size product estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal region this homologous site-specific DNA recombinases. Hybridization 3.6-kilobase EcoRI fragment carrying EcoRI-restricted chromosomal prepared from cells various stages showed that...
Fibrosis associated with chronic pancreatitis is an irreversible lesion that can disrupt pancreatic exocrine and endocrine function. Currently, there are no approved treatments for this disease. We previously showed siRNA against collagen-specific chaperone protein gp46, encapsulated in vitamin A-coupled liposomes (VA-lip-siRNAgp46), resolved fibrosis a model of liver cirrhosis. This treatment was investigated induced by dibutyltin dichloride (DBTC) cerulein rats.Specific uptake...
Summary Surface properties, such as adhesion and hydrophobicity, constrain dispersal of bacterial spores in the environment. In Bacillus subtilis , these properties are influenced by outermost layer spore, crust . Previous work has shown that two clusters, cotVWXYZ cgeAB encode protein components crust. Here, we characterize respective roles genes surface using Bacterial Adherence to Hydrocarbons assays, negative staining polysaccharides India ink Transmission Electron Microscopy. We showed...
In this study, we developed the electrical impedance method which simultaneously measures thickness and breakdown ratio of oil films in elastohydrodynamic (EHD) line contacts within thrust needle roller bearings. Initially, theoretically demonstrated that film can be measured using complex is produced when an AC voltage applied to EHD contacts. To verify measurement accuracy method, monitored a bearing compared it with theoretical value. The results revealed was thinner than value...
With most immunological reactions, tissue fibrosis, collagen overproduction caused by immune cytokines, is inevitably associated. Among the various heat shock protein 47 (HSP47) a procollagen-specific molecular chaperon and essential for secretion of procollagen from cells. Induction HSP47 TGF-beta has been previously reported in rat skeletal myoblasts mouse osteoblasts, but not human diploid fibroblasts. As IL-1beta, its effect on elucidated. In present study, using embryonic lung...
sur0B20 is a mutation that suppresses the effects of spo0B delta B or spo0F221 mutations in Bacillus subtilis, an allele spo0A gene (Glu-14 to Val-14 conversion) and restores sporulation spo0F mutants wild-type level. Here, we report isolation suppressor (ssb). One these mutations, ssb-12, severely impairs activity sur0B20. A 2.5-kbp MboI fragment which complements ssb-12 was cloned by prophage transformation method using phi CM as vector. Nucleotide sequencing revealed two open reading...
Volatile sulfur compounds are the main causing halitosis. One of these compounds, hydrogen sulfide (H(2)S), which is responsible for physiological halitosis, reported also to have periodontal pathogenic activities. Hydrogen has been shown activate apoptotic process in different tissues. Apoptosis plays an important role development periodontitis. The aim this study was determine whether H(2)S causes apoptosis human gingival epithelial cells and examine cellular signaling pathway initiating...