A5072247211- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Animal Virus Infections Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Malaria Research and Control
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- HIV Research and Treatment
- Dengue and Mosquito Control Research
- Animal Disease Management and Epidemiology
- Viral Infections and Outbreaks Research
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Invertebrate Immune Response Mechanisms
- Microbial infections and disease research
- Plant and Fungal Interactions Research
Ministério da Saúde
2024-2025
Universidade de Brasília
2024-2025
Universidade Federal do Pará
2018-2023
Ministero della Salute
2023
Diarrhea remains one of the most common causes deaths in children. Although many studies have investigated prevalence enteric pathogens around globe some diarrheal episodes remain unexplained. It is possible that yet-unidentified viral agents could be related to these cases gastroenteritis. By using metagenomics techniques, we screened 251 fecal samples children between 0.5 2.5-year-old with acute diarrhea not associated pathogens. These live rural areas and different levels contact animals...
Mosquito-borne diseases have a significant public health threat worldwide, with arboviruses accounting for high proportion of infectious and mortality annually. Brazil, in particular, has been suffering outbreaks transmitted by mosquito viruses, notably those the Aedes genus, such as dengue, Zika, chikungunya. Against this background, São Paulo Zoo is an intriguing ecological niche to explore virome mosquitoes, potentially shedding light on dynamics arbovirus transmission within confined...
Viruses are the most diverse and abundant microorganisms on earth, highly adaptive to a wide range of hosts. Viral diversity within invertebrate hosts has gained notoriety in recent years public health as several such viruses have been medical importance. Aedes aegypti serves vector for that caused epidemics last year throughout Brazil; including Dengue, Zika Chikungunya. This study aimed identify new viral agents mosquito city Amazonian region, where it is endemic. Metagenomic investigation...
Classical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of flavivirus. Remarkably dISFV evolutionarily related to some pathogenic flavivirus, such as Zika dengue viruses. The Evolutionary relatedness allowed us investigate evolutionary principle host adaptation. Additionally, can be used for development vaccines explore underlying principles mammalian pathogenicity. Here we describe genetic characterization a novel putative...
Enterovirus B73 is a new member of the B species. First detected in USA, it has been subsequently identified China, India, Oman, and Netherlands. In this study, we characterize first strain (named TO-127) to be South America. TO-127 was obtained from child with acute gastroenteritis living rural area Northern Brazil. The subject not infected any known enteric pathogens such as norovirus, rotavirus, helminths, or bacteria. Analysis nearly full-length genome (6993 nt) indicated 74–75%...
Metagenomics based on the next-generation sequencing (NGS) technique is a target-independent assay that enables simultaneous detection and genomic characterization of all viruses present in sample. There limited amount data about virome individuals with gastroenteritis (GI). In this study, enteric 250 (92% were children under 5 years old) GI living northeastern northern regions Brazil was characterized. Fecal samples subjected to NGS, metagenomic analysis virus-like particles (VLPs)...
Metagenomic studies of mosquito viromes demonstrated a more diverse composition than just an exclusive pathogenic arboviruses transmitted to humans. In our study, the virome 866 female mosquitoes collected throughout 2020 at São Paulo Zoo, located in city Paulo/SP—Brazil, was obtained. Specifically, this paper, we describe new virus found by viral RNA extraction and next-generation MiSeq sequencing group 23 specimens Anopheles (Nys.) strodei. The complete genome with length 9709 nucleotides...
Classical insect-specific flaviviruses (cISFs) have been widely detected in different countries the last decades. Here, we characterize near full-length genomes of two cISFs mosquitoes collected city Macapá, state Amapá, Amazon region Brazil. A total 105 pools female mosquitos were analyzed by next-generation sequencing (NGS). Comparative genomics and phylogenetic analysis identified three strains cell fusing agent virus (CFAV) Culex flavivirus (CxFV). All sequences obtained from sp., except...
Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity viruses (pathogens non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected virome analysis fecal samples from migratory birds Australia. We now report detection RNA-dependent RNA polymerase sequences HRLV pools Aedes aegypti Culex quinquefasciatus mosquitoes species Brazilian Amazon forest. Phylogenetic inferences indicated that all strains fall...
Echoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection identification echovirus genotypes clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences nine echoviruses, obtained by next-generation sequencing 238 fecal from individuals with gastroenteritis regions Brazil. Detected were classified into six...
Dengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology growing and increasingly invaluable tool for understanding dispersal, persistence, diversity of this impactful virus. In study, plasma samples (n = 824) from individuals with symptoms consistent an arboviral febrile illness were analyzed to identity molecular epidemiological dynamics DENV circulating in Brazilian state Amapá. Twelve type...
The simultaneous transmission of two lineages the chikungunya virus (CHIKV) was discovered after pathogen's initial arrival in Brazil. In Oiapoque (Amapá state, north Brazil), Asian lineage (CHIKV-Asian) discovered, while Bahia East-Central-South-African (CHIKV-ECSA) (northeast Brazil). Since then, CHIKV-Asian has been restricted to Amazon region (mostly state Amapá), whereas ECSA expanded across country. Despite fact that already present region, brought from northeast caused a large...
Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources viruses. particular, 251 fecal samples obtained from individuals were sequenced southern, northeastern, northern Brazil. all also analyzed using culture...
The genus totivirus in the family Totiviridae contains double-stranded RNA viruses. Their genome has two open reading frames (ORFs) that encode capsid protein (CP) and RNA-dependent polymerase (RdRp). toti-like viruses recently identified Anopheles sp. Aedes aegypti mosquitoes (AaTV) share same organization as other totiviruses. AaTVs have been described distinct geographical regions are monophyletic. In this study, we show AaTV sequences can be grouped into at least three phylogenetic...
Abstract The interaction between the dengue virus (DENV) and human dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) receptor plays a crucial role in viral entry infection. Understanding dynamics of this its evolution over time is essential for comprehending host-virus relationship emergence strains with increased pathogenicity. In study, we employed phylogenetic filtering to provide structural approach investigate co-evolutionary patterns DENV...