A5057245111- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Legume Nitrogen Fixing Symbiosis
- Antibiotic Resistance in Bacteria
- Environmental Science and Technology
- Ecology, Conservation, and Geographical Studies
- Agriculture, Plant Science, Crop Management
- Community Development and Social Impact
- German legal, social, and political studies
- Biosensors and Analytical Detection
- FinTech, Crowdfunding, Digital Finance
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Bacterial Genetics and Biotechnology
- Iron oxide chemistry and applications
- European Union Policy and Governance
- European Politics and Security
- Cooperative Studies and Economics
- Plant responses to elevated CO2
- Climate Change and Environmental Impact
- Agriculture and Biological Studies
- Plant Micronutrient Interactions and Effects
University of Zurich
2012-2022
Leibniz Institute of Plant Genetics and Crop Plant Research
2013
Although iron (Fe) is one of the most abundant elements in earth's crust, its low solubility soils restricts Fe uptake by plants. Most plant species acquire acidifying rhizosphere and reducing ferric to ferrous prior membrane transport. However, it unclear how these plants access cope with high soil pH. In a mutant screening, we identified 2-oxoglutarate-dependent dioxygenase Feruloyl-CoA 6′-Hydroxylase1 (F6′H1) be essential for tolerance Arabidopsis (Arabidopsis thaliana) pH-induced...
Many bacterial pathogens produce diffusible signal factor (DSF)-type quorum sensing (QS) signals in modulation of virulence and biofilm formation. Previous work on Xanthomonas campestris showed that the RpfC/RpfG two-component system is involved responding to DSF signals, but little known other microorganisms. Here we show Burkholderia cenocepacia DSF-family cis -2-dodecenoic acid (BDSF) negatively controls intracellular cyclic dimeric guanosine monophosphate (c-di-GMP) level through a...
Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible factor cis-2-dodecenoic acid (BDSF). Previous studies have shown AHLs BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity pathogenicity. In this study we mapped stimulon by RNA-Seq shotgun proteomics analysis. We demonstrate a set of identified BDSF-regulated genes or...
Burkholderia cenocepacia has emerged as an important pathogen for patients suffering from cystic fibrosis (CF). Previous work shown that this organism employs the CepIR quorum-sensing (QS) system to control expression of virulence factors well formation biofilms. To date, however, very little is known about QS-regulated and virtually nothing link QS biofilm formation. Here, we have employed a combined transcriptomic proteomic approach precisely define regulon in our model strain B. H111, CF...
ABSTRACT Members of the genus Burkholderia are versatile bacteria capable colonizing highly diverse environmental niches. In this study, we investigated global response opportunistic pathogen cenocepacia H111 to nitrogen limitation at transcript and protein expression levels. addition a classical starvation, including activation glutamine synthetase, PII proteins, two-component regulatory system NtrBC, B. also upregulated polyhydroxybutyrate (PHB) accumulation exopolysaccharide (EPS)...
ABSTRACT The Burkholderia cepacia complex (BCC) is a group of related bacterial species that are commonly isolated from environmental samples. Members the BCC can cause respiratory infections in cystic fibrosis patients and immunocompromised individuals. We report here genome sequence cenocepacia H111, well-studied model strain BCC.
The opportunistic human pathogen Burkholderia cenocepacia H111 uses two chemically distinct signal molecules for controlling gene expression in a cell density-dependent manner: N-acyl-homoserine lactones (AHLs) and cis-2-dodecenoic acid (BDSF). Binding of BDSF to its cognate receptor RpfR lowers the intracellular c-di-GMP level, which turn leads differential target genes. In this study we analysed transcriptional profile B. upon artificially altering cellular level. One hundred eleven genes...
Burkholderia cenocepacia H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of B. are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In present study we analyzed role all 25 putative c-di-GMP metabolizing proteins respect to motility, colony morphology, pellicle biofilm virulence. We found that RpfR a key regulator signaling cenocepacia, affecting broad spectrum...
In Burkholderia cenocepacia H111, the large surface protein BapA plays a crucial role in formation of highly structured communities, known as biofilms. We have recently demonstrated that quorum sensing (QS) is necessary for maximal expression bapA. this study we identify BapR, from IclR family transcriptional regulators that, conjunction with QS, controls biofilm by affecting present evidence addition to bapA, BapR influences extracellular proteases, swimming motility and has profound impact...
Abstract The polysaccharide Bep is essential for in vitro biofilm formation of the opportunistic pathogen Burkholderia cenocepacia . We found that diffusible signaling factor (BDSF) quorum sensing receptor RpfR a negative regulator bep gene cluster B. An rpfR mutant formed wrinkled colonies, whereas additional mutations genes or known regulators like berA and berB restored wild-type smooth colony morphology. there good correlation between intracellular c-di-GMP levels expression when level...
Abstract The polysaccharide Bep is essential for in vitro biofilm formation of the opportunistic pathogen Burkholderia cenocepacia . We found that BDSF quorum sensing receptor RpfR a negative regulator bep gene cluster B. cenocepacia. An rpfR mutant formed wrinkled colonies, whereas additional mutations genes or known regulators such as berA and berB restored smooth colony morphology wild-type. In accordance with previous work, we there good correlation between intracellular c-di-GMP levels...