A5041981028- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Neonatal Respiratory Health Research
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Tracheal and airway disorders
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Inhalation and Respiratory Drug Delivery
- RNA and protein synthesis mechanisms
- Pediatric health and respiratory diseases
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Congenital Ear and Nasal Anomalies
- Probiotics and Fermented Foods
- Immunodeficiency and Autoimmune Disorders
- Drug Transport and Resistance Mechanisms
- Antibiotic Use and Resistance
- Legume Nitrogen Fixing Symbiosis
- Asthma and respiratory diseases
- Nosocomial Infections in ICU
Medizinische Hochschule Hannover
2016-2025
German Center for Lung Research
2016-2025
German Center for Infection Research
2023
Heidelberg University
2014
British Columbia Children's Hospital
2014
University of British Columbia
2014
University of Tübingen
2014
Child and Family Research Institute
2014
Heidelberg University
2014
University Hospital Heidelberg
2014
Summary Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as biosafety host for the cloning of foreign genes. The also considerable potential biotechnological applications. Sequence analysis 6.18 Mb genome strain KT2440 reveals diverse transport and metabolic systems. Although there high level conservation with pathogenic Pseudomonad aeruginosa (85% predicted coding regions are shared), key virulence factors including exotoxin A type III...
Bacteria with intrinsic resistance to antibiotics are a worrisome health problem. It is widely believed that antibiotic of bacterial pathogens mainly the consequence cellular impermeability and activity efflux pumps. However, analysis transposon-tagged Pseudomonas aeruginosa mutants presented in this article shows phenotype emerges from action numerous proteins all functional categories. Mutations some genes make P. more susceptible thereby represent new targets. other resistant therefore...
The metabolically versatile Gram-negative bacterium Pseudomonas aeruginosa inhabits terrestrial, aquatic, animal-, human-, and plant-host-associated environments is an important causative agent of nosocomial infections, particularly in intensive-care units. population genetics P. was investigated by approach that generally applicable to the rapid, robust, informative genotyping bacteria. DNA, amplified from bacterial colony circles multiplex primer extension, hybridized onto a microarray...
The Pseudomonas aeruginosa genome (G + C content 65-67%, size 5.5 – 7 Mbp) is made up of a single circular chromosome and variable number plasmids. Sequencing complete genomes or blocks the accessory has revealed that encodes large repertoire transporters, transcriptional regulators two-component regulatory systems which reflects its metabolic diversity to utilize broad range nutrients. conserved core component largely collinear among P. strains exhibits an interclonal sequence 0.5 0.7%....
Rationale: The CFTR (cystic fibrosis transmembrane conductance regulator) modulator combination elexacaftor/tezacaftor/ivacaftor (ELX/TEZ/IVA) was shown to improve clinical outcomes and sweat chloride concentration in patients with cystic (CF) one or two F508del alleles. However, the effect of ELX/TEZ/IVA on function airways intestine has not been studied. Objectives: To assess airway intestinal epithelia CF alleles aged 12 years older. Methods: This prospective, observational, multicenter...
Rationale: We recently demonstrated that triple-combination CFTR (cystic fibrosis transmembrane conductance regulator) modulator therapy with elexacaftor/tezacaftor/ivacaftor (ELX/TEZ/IVA) improves function in airway and intestinal epithelia to 40–50% of normal patients cystic (CF) one or two F508del alleles. In previous studies, this improvement was shown improve clinical outcomes; however, effects on the lung clearance index (LCI) determined by multiple-breath washout abnormalities...
The genomic relatedness of 573 Pseudomonas aeruginosa strains from environmental and clinical habitats was examined by digesting the genome with rare-cutting enzyme SpeI. Thirty-nine were collected mainly aquatic origin, like rivers, lakes, or sanitary facilities. Four hundred fifty 76 patients cystic fibrosis (CF) treated at four different centers, 25 additional isolates suffering other diseases. Twenty-nine P. environment one CF clinic. Thirty culture collections clinic origin. A common...
Heterologous expression of the cystic fibrosis transmembrane conductance regulator (CFTR) provided evidence that major (CF) mutation ΔF508 leads to defective protein folding in endoplasmic reticulum, which prevents its processing and targeting cell surface. In this study, we investigated endogenous CFTR skin biopsies respiratory intestinal tissue specimens from homozygous non-CF patients, using immunohistochemical immunoblot analyses with a panel antibodies. was detected at luminal surface...
Pseudomonas aeruginosa PAO1 is the most commonly used strain for research on this ubiquitous and metabolically versatile opportunistic pathogen. Strain PAO1, a derivative of original Australian PAO isolate, has been distributed worldwide to laboratories collections. Over decades discordant phenotypes sublines have emerged. Taking existing PAO1-UW genome sequence (named after University Washington, which led sequencing project) as blueprint, sequences reference strains MPAO1 PAO1-DSM (stored...
The epidemiology of chronic colonization airways with Pseudomonas aeruginosa was monitored in 44 patients cystic fibrosis (CF) by DraI/SpeI macrorestriction analyses 489 isolates. Sequential P. isolates (144) that had been collected from 32 CF over < or = 2.5 years were investigated, and 12 followed for 8 after onset colonization. Forty-eight different genotypes uncovered 481 typeable Ten found > 1 unrelated patient. 6 most frequent clones identified 58% the harboring their initially...
Pseudomonas aeruginosa utilizes a number of distinct pathways to secrete proteins that play various roles during infection. These include the type II secretion system, which is responsible for majority exoproducts into surrounding environment, including toxins and degradative enzymes. In contrast, III system mediates delivery protein effectors directly cytoplasm host cell. Using tissue culture assays mouse acute-pneumonia model, we have determined contribution each strain PAK, ExoS major...
The epidemiology of Pseudomonas aeruginosa infection at a cystic fibrosis (CF) center was monitored over 3-year period. A total 835 isolates from 72 unrelated patients and 22 siblings with CF were analyzed by genome fingerprinting serotyping, bacteriophage typing, pyocin typing. For fingerprinting, bacterial chromosomes digested one the restriction endonucleases SpeI, DraI, XbaI, SspI, NheI, which cut only rarely, subsequently separated field inversion gel electrophoresis. physical analysis...
During long-term lung infection in patients with cystic fibrosis (CF), Pseudomonas aeruginosa strains develop mutations leading to clonal expansion. This microevolution is believed be correlated a reduced virulence.We tested this hypothesis models of infection, using mice different genetic backgrounds.From infected airways six CF, 25 P. clones were isolated during period up 16.3 years and genotypically phenotypically characterized. Virulence the 8 early, 6 intermediate, 11 late CF isolates 5...
In the context of chronic lung infection due to Pseudomonas aeruginosa in cystic fibrosis (CF), attention has been focused on presence most common mucoid phenotype. this study, small-colony variants (SCVs) P. respiratory tract specimens from patients with CF was investigated, and clinical conditions predisposing SCVs were analyzed. isolated 33 86 aeruginosa-positive over a 2-year period. Fast-growing revertants larger surface colonies could be SCV populations. Electron microscopy revealed no...
Pseudomonas aeruginosa, an opportunistic human pathogen and ubiquitous environmental bacterium, is capable of forming specialized bacterial communities, referred to as biofilm. The results this study demonstrate that the unique environment cystic fibrosis (CF) lung seems select for a subgroup autoaggregative hyperpiliated P. aeruginosa small-colony variants (SCVs). These morphotypes showed increased fitness under stationary growth conditions in comparison with clonal wild-types fast-growing...
ABSTRACT The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is a transmissible aggressive pathogen cystic fibrosis (CF) patients. We compared transcriptome profiles two LES isolates with each other and laboratory genetic reference (PAO1) after growth to late exponential phase following exposure oxidative stress. Both exhibited enhanced antimicrobial resistances linked specific mutations in efflux pump genes. Although transcription AmpC β-lactamase was up-regulated both, one...
<h3>Rationale:</h3> Lower airway (LAW) infection with <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> is the leading cause of morbidity in cystic fibrosis (CF). The upper airways (UAW) were shown to be a gateway for acquisition opportunistic bacteria act as reservoir them. Therefore, tools UAW assessment within CF routine care require evaluation. <h3>Objectives:</h3> aims study non-invasive LAW microbial colonisation, genotyping <i>P <i>S strains from both segments....
The influence of ciprofloxacin, nalidixic acid, norfloxacin, novobiocin, and ofloxacin on elements eucaryotic DNA replication was investigated in vitro. Each the 4-quinolones, when present amounts more than 100 micrograms/ml, reversibly inhibited synthesis performed by 95 polymerase alpha primase complex from calf thymus. Novobiocin at 500 micrograms/ml or higher concentrations irreversibly inactivated complex. accuracy vitro absence repair mechanisms determined amber-revertant assays with...
<ns4:p>The versatile and ubiquitous<ns4:italic>Pseudomonas aeruginosa</ns4:italic>is an opportunistic pathogen causing acute chronic infections in predisposed human subjects. Here we review recent progress understanding<ns4:italic>P. aeruginosa</ns4:italic>population biology virulence, its cyclic di-GMP-mediated switches of lifestyle, interaction with the mammalian host as well role type III VI secretion systems in<ns4:italic>P. aeruginosa</ns4:italic>infection.</ns4:p>
Summary Clones C and PA14 are the worldwide most abundant clonal complexes in Pseudomonas aeruginosa population. The microevolution of clones was investigated serial cystic fibrosis (CF) airway isolates collected over 20 years since onset colonization. Intraclonal evolution CF lungs resolved by genome sequencing first, intermediate late subsequent multimarker SNP genotyping whole strain panel. Mapping sequence reads onto P. reference unravelled an intraclonal interclonal diversity 0.0035%...