A5047674365- Olfactory and Sensory Function Studies
- Proteoglycans and glycosaminoglycans research
- Memory and Neural Mechanisms
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- EEG and Brain-Computer Interfaces
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Cephalopods and Marine Biology
- Neuropeptides and Animal Physiology
- Neural dynamics and brain function
- RNA regulation and disease
- Cell Image Analysis Techniques
- Advanced Chemical Sensor Technologies
- Virus-based gene therapy research
- Lipid Membrane Structure and Behavior
- Underwater Acoustics Research
University of Oslo
2021-2025
University of Bergen
2018-2020
Genetically encoded Ca
Abstract Perineuronal nets (PNNs) are a condensed form of extracellular matrix primarily found around parvalbumin-expressing (PV+) interneurons. The postnatal maturation PV+ neurons is accompanied with the formation PNNs and reduced plasticity. Alterations in PNN neuron function have been described for mental disorders such as schizophrenia autism. highly dependent on aggrecan, proteoglycan encoded by ACAN gene, but it remains unknown if produced themselves. Thus, we established knockout...
Activity‐regulated cytoskeleton‐associated protein (Arc) is a interaction hub with diverse roles in intracellular neuronal signaling, and important functions synaptic plasticity, memory, postnatal cortical development. Arc has homology to retroviral Gag capable of self‐assembly into virus‐like capsids implicated the intercellular transfer RNA. However, molecular basis self‐association capsid formation largely unknown. Here, we identified 28‐amino‐acid stretch mammalian N‐terminal (NT) domain...
Abstract The activity‐regulated cytoskeleton‐associated protein ( ARC ) is critical for long‐term synaptic plasticity and memory formation. Acting as a interaction hub, regulates diverse signalling events in postsynaptic neurons. A site present the C‐terminal domain CTD ), bilobar structure homologous to retroviral Gag capsid domain. We hypothesized that detailed knowledge of three‐dimensional molecular monomeric full‐length crucial understand its function; therefore, we set out determine...
Abstract The transition from juvenile to adult is accompanied by the maturation of inhibitory parvalbumin-positive (PV+) neurons and reduced plasticity. This involves formation perineuronal nets (PNNs), a dense configuration extracellular matrix that predominantly envelops neurons. Aggrecan, proteoglycan encoded ACAN gene, has been shown have key role in PNNs as knock-out brain reactivates plasticity, but contribution different cell populations unknown Here, we establish characterize mouse...
The perineuronal nets (PNNs) are sugar coated protein structures that encapsulate certain neurons in the brain, such as parvalbumin positive (PV) inhibitory neurons. As PNNs theorized to act a barrier ion transport, they may effectively increase membrane charge-separation distance, thereby affecting capacitance. Tewari et al. (2018) found degradation of induced 25%-50% capacitance [Formula: see text] and reduction firing rates PV-cells. In current work, we explore how changes affects rate...
The medial entorhinal cortex (MEC) is pivotal in spatial computations and episodic memory. In particular, an animal’s position can be decoded from the activity of grid cells. However, it remains elusive whether MEC could play a more general role different types associative learning how representations develop during process. It has been shown that postrhinal (POR), which directly connected to MEC, integrates visual stimuli with salient outcomes. Here, we use non-spatial association task...
The medial entorhinal cortex (MEC) is pivotal in spatial computations and episodic memory. In particular, an animal’s position can be decoded from the activity of grid cells. However, it remains elusive whether MEC could play a more general role different types associative learning how representations develop during process. It has been shown that postrhinal (POR), which directly connected to MEC, integrates visual stimuli with salient outcomes. Here, we use non-spatial association task...
Abstract Studies of body-to-brain communication often examine one stimulus or organ at a time, yet the brain must integrate many body signals during behavior. For example, food consumption generates diverse oral and post-oral chemical mechanical transduced by well-characterized peripheral neuronal pathways. Far less is known about how these other bodily are integrated organized in brainstem lateral parabrachial nucleus (LPBN), key interoceptive sensory hub. We established methods to image...
Highly salient events activate neurons across various brain regions. During subsequent rest or sleep, the activity patterns of these often correlate with those observed during preceding experience. Growing evidence suggests that reactivations play a crucial role in memory consolidation, process by which experiences are solidified cortical networks for long-term storage. Here, we use longitudinal two-photon Ca 2+ imaging alongside paired LFP recordings hippocampus and cortex, to show targeted...
ABSTRACT Expression of activity-regulated cytoskeleton-associated protein (Arc) is critical for long-term synaptic plasticity, memory formation, and cognitive flexibility. The ability Arc to self-associate form virus-like capsid structures implies functionally distinct oligomeric states. However, the molecular mechanism oligomerization unknown. Here, we identified a 28-amino-acid region necessary sufficient oligomerization. This located within second coil predicted anti-parallel coiled-coil...
ABSTRACT Calcium imaging using genetically encoded Ca 2+ indicators (GECIs) is a widely adopted method to measure neural activity in modern neuroscience. Here, we explore the use of systemically administered viral vectors for brain-wide expression GECIs, and adapt novel GECIs optimize signal-to-noise. We show that systemic injections PHP.eB AAVs express highly promising technique circumvent need transgenic GECI expressing mouse lines. also establish soma-targeted outperform current both...
Abstract Highly salient events activate neurons across various brain regions. During subsequent rest or sleep, the activity patterns of these often correlate with those observed during preceding experience. Growing evidence suggests that reactivations play a crucial role in memory consolidation, process by which experiences are solidified cortical networks for long-term storage. Here, we demonstrate lateral visual cortex vital consolidation association learning. By employing longitudinal...
Abstract The medial entorhinal cortex (MEC) is pivotal in spatial computations and episodic memory. In particular, an animal’s position can be decoded from the activity of grid cells. However, it remains elusive whether MEC could play a more general role different types associative learning how representations develop during process. It has been shown that postrhinal (POR), which directly connected to MEC, integrates visual stimuli with salient outcomes. Here, we use non-spatial association...
Abstract The activity-regulated cytoskeleton-associated protein (ARC) is critical for long-term synaptic plasticity and memory formation. Acting as a interaction hub, ARC regulates diverse signalling events in postsynaptic neurons. A site present the C-terminal domain (CTD), bilobar structure homologous to retroviral Gag capsid domain. However, knowledge of 3-dimensional full-length required elucidate its molecular function. We purified recombinant monomeric analyzed using small-angle X-ray...
Abstract The perineuronal nets (PNNs) are sugar coated protein structures that encapsulate certain neurons in the brain, such as parvalbumin positive (PV) inhibitory neurons. As PNNs theorized to act a barrier ion transport, they may effectively increase membrane charge-separation distance, thereby affecting capacitance. Tewari et al. (2018) found degradation of induced 25\%-50\% capacitance and reduction firing rates PV-cells. In current work, we attempted reproduce experiments by using...