A5042070963- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Genetics, Aging, and Longevity in Model Organisms
- Developmental Biology and Gene Regulation
- Machine Learning in Healthcare
- Lanthanide and Transition Metal Complexes
- Medical Imaging Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- RNA Research and Splicing
- Scientific Computing and Data Management
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Plant Gene Expression Analysis
- Gene Regulatory Network Analysis
- Distributed and Parallel Computing Systems
- Light effects on plants
- Microtubule and mitosis dynamics
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Musculoskeletal pain and rehabilitation
- Genetics and Neurodevelopmental Disorders
- Plant Surface Properties and Treatments
- Myofascial pain diagnosis and treatment
Princeton University
2018-2023
University of Lisbon
2023
University of Minho
2013-2018
University of Aveiro
2018
John Innes Centre
2013
Past studies offer contradictory claims for the role of genome organization in regulation gene activity. Here, we show through high-resolution chromosome conformation analysis that
The establishment of meristematic domains with different transcriptional activity is essential for many developmental processes. asymmetry the Antirrhinum majus flower established by transcription factors an asymmetric pattern activity. To understand how this asymmetrical established, we studied molecular mechanism through which dorsal MYB protein RADIALIS (RAD) restricts factor DIVARICATA (DIV) to ventral region meristem. We show that RAD competes DIV binding other MYB-like proteins, termed...
Developmental patterning networks are regulated by multiple inputs and feedback connections that rapidly reshape gene expression, limiting the information can be gained solely from slow genetic perturbations. Here we show fast optogenetic stimuli, real-time transcriptional reporters, a simplified background combined to reveal kinetics of expression downstream developmental transcription factor in vivo. We engineer light-controlled versions Bicoid study their effects on gap genes embryos. Our...
Significance At the mid-blastula transition, rapid cell divisions are slowed down and global activation of zygotic genes occur, preparing early embryo for differentiation further development. Using live imaging embryos with reduced ploidy cycle lengths, we examined effects nuclear content to cytoplasm (N/C) ratio on timing genome activation. While some predominantly affected by N/C ratio–dependent changes in durations, other demonstrate direct regulation through transcriptional kinetics...
Cork oak (Quercus suber) is one of the rare trees with ability to produce cork, a material widely used make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms cork formation are still poorly understood, in great part due difficulty studying species long life-cycle for which there scarce molecular/genomic information. forests ecological importance represent major economic social resource Southern Europe Northern Africa. However, global...
The aims of this study are to investigate the association between: (i) forward head posture (FHP) and pressure pain thresholds (PPTs); (ii) FHP maladaptive cognitive processes; (iii) neck characteristics in university students with subclinical pain.A total 140 students, 90 asymptomatic 50 pain, entered study. Demographic data, anthropometric FHP, PPTs were collected for both groups. In addition, characteristics, catastrophizing, fear movement assessed participants pain. was characterized by...
The comparison of closely related taxa is cornerstone in biology, as understanding mechanisms leading up to differentiation relation extant shared characters are powerful tools interpreting the evolutionary process. Hotspots biodiversity such west-Mediterranean, where many lineages meet ideal grounds study these processes. We set explore interesting example Sooty Copper butterflies: widespread Eurasian Lycaena tityrus (Poda, 1761) comes into contact Iberia with and local endemic, L. bleusei...
The establishment of new interactions between transcriptional regulators increases the regulatory diversity that drives phenotypic novelty. To understand how such evolve, we have studied a module (DDR) composed by three MYB-like proteins: DIVARICATA (DIV), RADIALIS (RAD), and DIV-and-RAD-Interacting Factor (DRIF). DIV DRIF proteins form complex is disrupted in presence RAD, small interfering peptide, due to formation RAD-DRIF dimers. This dynamic interaction result molecular switch mechanism...
To enable large-scale analyses of transcription regulation in model species, we developed DeepArk, a set deep learning models the cis -regulatory activities for four widely studied species: Caenorhabditis elegans , Danio rerio Drosophila melanogaster and Mus musculus . DeepArk accurately predicts presence thousands different context-specific regulatory features, including chromatin states, histone marks, factors. In vivo studies show that can predict impact any genomic variant (including...
Abstract To enable large-scale analyses of regulatory logic in model species, we developed DeepArk ( https://DeepArk.princeton.edu ), a set deep learning models the cis -regulatory codes four widely-studied species: Caenorhabditis elegans, Danio rerio , Drosophila melanogaster and Mus musculus . accurately predicts presence thousands different context-specific features, including chromatin states, histone marks, transcription factors. In vivo studies show that can predict impact any genomic...
Abstract Developmental patterning networks are regulated by multiple inputs and feedback connections that rapidly reshape gene expression, limiting the information can be gained solely from slow genetic perturbations. Here we show fast optogenetic stimuli, real-time transcriptional reporters, a simplified background combined to reveal quantitative regulatory dynamics complex network in vivo . We engineer light-controlled variants of Bicoid transcription factor study their effects on...
Abstract Early embryos must rapidly generate large numbers of cells to form an organism. Many species accomplish this through a series rapid, reductive, and transcriptionally silent cleavage divisions. Previous work has demonstrated that the number divisions before both cell cycle elongation zygotic genome activation (ZGA) is regulated by ratio nuclear content cytoplasm (N/C). To understand how N/C affects timing ZGA, we directly assayed behavior several previously identified...