Abstract Background Grading of cancer histopathology slides requires more pathologists and expert clinicians as well it is time consuming to look manually into whole-slide images. Hence, an automated classification histopathological breast sub-type useful for clinical diagnosis therapeutic responses. Recent deep learning methods medical image analysis suggest the utility radiologic imaging relating disease characteristics or patient stratification. Methods To develop a hybrid model using...

Abstract Background This study focused on the drought tolerance and resilience of two ash species: Fraxinus chiisanensis F. rhynchophylla . These species are distributed in different habitats, suggesting that they have levels tolerance. Understanding their response to stress, particularly during seedling stage, is crucial for selecting developing drought-resistant varieties. aimed compare phenotypic, physiological, transcriptomic characteristics drought-stressed recovered rewatered plants a...

Anti-apoptotic proteins such as BCL-2, BCL-XL and MCL-1 bind with pro-apoptotic to induce apoptosis mechanism. BCL-2 family are key regulators of process. Over expression these anti-apoptotic lead several cancers by preventing apoptosis. A number studies revealed that ginseng derivatives reduce tumor growth. Ginseng, the most valuable medicinal herb found in eastern Asia belongs Araliaceae family. In this study, docking simulations were performed for ginsenosides from Panax ginseng. Our...

Plasmodiophora brassicae is a soil-borne plant pathogen that causes clubroot disease, which results in crop yield loss cultivated Brassica species. Here, we investigated whether quantitative trait locus (QTL) B. rapa might confer resistance to Korean P. pathotype isolate, Seosan. We crossed resistant and susceptible parental lines analyzed the segregation pattern F2 population of 348 lines. identified mapped novel QTL using same mapping included Chinese cabbage turnip Forty-five 45 along...

Plants, as sessile organisms, can suffer serious growth and developmental consequences under cold stress conditions. Glutathione transferases (GSTs, EC 2.5.1.18) are ubiquitous multifunctional conjugating proteins, which play a major role in responses by preventing oxidative damage reactive oxygen species (ROS). Currently, understanding of their function(s) during different biochemical signaling pathways condition remain unclear. In this study, using combined computational strategy, we...

Panax ginseng Meyer has been shown to be effective in mitigating various diseases. Protopanaxadiols (PPD) and protopanaxatriols (PPT), which are the main constituents of ginseng, have impact obesity. Therefore, we selected several important ginsenosides perform our docking study determine if they had binding affinity with peroxisome proliferator activated receptor gamma (PPARγ), is a major transcription factor adipocytes. Among them, only few demonstrated PPARγ. Other than ginsenoside F2...

Powdery mildew is one of the most common fungal diseases in world. This disease frequently affects melon (Cucumis melo L.) and other Cucurbitaceous family crops both open field greenhouse cultivation. One goals genomics to identify polymorphic loci responsible for variation phenotypic traits. In this study, powdery assessment scores were calculated four accessions, 'SCNU1154', 'Edisto47', 'MR-1', 'PMR5'. To investigate genetic these whole genome re-sequencing using Illumina HiSeq 2000...

Various studies have demonstrated that overexpression of cathepsin K (Cat-K) causes excessive bone loss, which ultimately leads to a variety diseases including osteoporosis. Therefore, inhibition Cat-K signifies potential therapeutic target in osteoporosis treatment. Ginsenoside Rg3 is one the most promising compound Panax ginseng Meyer (P. ginseng) with numerous biological activities. Thus, recent study inhibitory effect isolated from P. was investigated order impede osteoclast activity by...

Context: Alzheimer's disease (AD) is the most common form of dementia affecting aged population and neuroinflammation one observed AD pathologies. NF-κB central regulator inflammation inhibitor κB kinase (IKK) converging point in activation. Celastrol a natural triterpene used as treatment for inflammatory conditions.Objective: This study determines neuroprotective inhibitory effect celastrol on amyloid beta1-42 (Aβ1-42) induced cytotoxicity IKKβ activity, respectively.Materials methods:...

Abstract In this study, two different hybrids of Easter lily ( Lilium longiflorum) , obtained from cross combinations, along with their four parents were sequenced by high–throughput RNA–sequencing (RNA–Seq) to find out differentially expressed gene in parent-hybrid combinations. The leaf mRNA profiles and RNA–sequenced a view identify the potential candidate genes related plant height heterosis. both based morphological traits mid–parent heterosis (MPH) was higher than high–parent (HPH) for...

Bulb onion (Allium cepa) is the second most widely cultivated and consumed vegetable crop in world. During winter, cold injury can limit production of bulb onion. Genomic resources available for are still very limited. To date, no studies on heritably durable freezing tolerance have been carried out genotypes. We applied high-throughput sequencing technology to (2°C), (-5 -15°C), control (25°C)-treated samples tolerant (CT) susceptible (CS) genotypes A. cepa lines. A total 452 million...

Lilies (Lilium sp.) are commercially important horticultural crops widely cultivated for their flowers and bulbs. Here, we conducted large-scale data mining of the lily transcriptome to develop transcription factor (TF)-associated microsatellite markers (TFSSRs). Among 216,768 unigenes extracted from our sequence data, 6966 harbored simple repeats (SSRs). Seventy-one SSRs were associated with TF genes, these used design primers validate potential as markers. These 71 accomplished 31...

Onion (Allium cepa L.) is an economically important vegetable crop around the world. Genetic and genomic research into various onion accessions will provide insights genome to enhance breeding strategies improve crops. However, onion's large size means that studies of molecular markers are limited in onion. This study aimed discover high quality single nucleotide polymorphisms (SNPs) from 192 inbred lines relating short-day cultivation Korea. Paired-end (PE) double digested restriction...

Lily belongs to family liliaceae, which mainly propagates vegetatively. Therefore, sufficient number of polymorphic, informative, and functional molecular markers are essential for studying a wide range genetic parameters in Lilium species. We attempted develop, characterize design SSR (simple sequence repeat) using online resources analyzing diversity population structure found di-nucleotide repeat motif were more frequent (4684) within 0.14 gb (giga bases) transcriptome than other repeats,...

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is crucial in plant metabolism and responses to various abiotic stresses. In the glycolysis pathway, glyceraldehyde-3-phosphate (G3P) oxidized 1,3-bisphosphate glycerate (1,3-BPG) through catalytic action of GAPDH. However,