A5040393482- Allergic Rhinitis and Sensitization
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Lichen and fungal ecology
- Asthma and respiratory diseases
- Indoor Air Quality and Microbial Exposure
- Forest Insect Ecology and Management
- Olfactory and Sensory Function Studies
- Plant and animal studies
- Plant nutrient uptake and metabolism
- Plant Pathogens and Fungal Diseases
- Plant Micronutrient Interactions and Effects
- Respiratory Support and Mechanisms
- Climate Change and Health Impacts
- Species Distribution and Climate Change
- Air Quality and Health Impacts
- Food Allergy and Anaphylaxis Research
- Entomological Studies and Ecology
- Contact Dermatitis and Allergies
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- COVID-19 and healthcare impacts
- Respiratory and Cough-Related Research
- Plant responses to elevated CO2
Leiden University Medical Center
2013-2024
University of Amsterdam
1987-2023
Amsterdam University Medical Centers
2023
Emma Kinderziekenhuis
2023
Leiden University
1991-2021
University of Tennessee at Knoxville
1991
Experimental Station
1991
Auburn University
1991
Tulane University
1991
Utrecht University
1989
A progressive global increase in the burden of allergic diseases has affected industrialized world over last half century and been reported literature. The clinical evidence reveals a general both incidence prevalence respiratory diseases, such as rhinitis (common hay fever) asthma. Such phenomena may be related not only to air pollution changes lifestyle, but also an actual airborne quantities allergenic pollen. Experimental enhancements carbon dioxide (CO) have demonstrated pollen amount...
Pollen monitoring is an important and widely used tool in allergy research creation of awareness pollen-allergic patients. Current pollen methods are microscope-based, labour intensive cannot identify to the genus level some relevant allergenic plant groups. Therefore, a more efficient, cost-effective sensitive method needed. Here, we present for identification quantification airborne using DNA sequencing. collected from ambient air standard techniques. extracted pollen, fragment chloroplast...
Abstract Invasive alien species (IAS) can substantially affect ecosystem services and human well-being. However, quantitative assessments of their impact on health are rare the benefits implementing IAS management likely to be underestimated. Here we report effects allergenic plant Ambrosia artemisiifolia public in Europe potential accidentally introduced leaf beetle Ophraella communa number patients healthcare costs. We find that, prior establishment O. , some 13.5 million persons suffered...
The role of motility in the colonization potato roots by Pseudomonas bacteria was studied. Four Tn5-induced flagella-less mutants plant-growth-stimulating P. fluorescens WCS374 appeared to be impaired their ability colonize growing roots.
In this work the bio-availability of amino acids for root-colonizing Pseudomonas fluorescens strain WCS365 in tomato rhizosphere was studied. The acid composition axenically collected root exudate determined. results show that aspartic acid, glutamic isoleucine, leucine, and lysine are major components. concentrations individual gnotobiotically grown plants were estimated considered to be too low support growth micro-organisms numbers usually found rhizosphere. To test experimentally,...
Abstract Background The EC ‐funded E uro P revall project examined the prevalence of food allergy across urope. A well‐established factor in occurrence is primary sensitization to pollen. Objective To analyse geographic and temporal variations pollen exposure, allowing investigation how these influence incidence allergies Methods Airborne data for two decades (1990–2009) were obtained from 13 monitoring sites located as close possible survey centres. Start dates, intensity duration B...
As an approach to understanding the molecular basis of reduction in plant yield depression by root-colonizing Pseudomonas spp. and especially role bacterial cell surfaces this process, we characterized 30 plant-root-colonizing with respect siderophore production, antagonistic activity, plasmid content, sodium dodecyl sulphate-polyacrylamide gel electrophoresis patterns their envelope proteins. The results showed that all strains produce hydroxamate-type siderophores which, because...
An extensive colonization of the endorhizosphere by fluorescent pseudomonads was observed in tomato plants grown on artificial substrates. These studies reveal that a significantly higher percentage obtained from (30%) reduced plant growth than those root surface (4%). Lipopolysaccharide patterns, cell envelope protein and other biochemical characteristics indicated Pseudomonas isolates are distinct surface. Isolates especially were able to recolonize both sterile nonsterile roots. The...
Hay fever patients can be helped in managing their symptoms by predicting the severity of upcoming grass pollen seasons.
Efficient colonization of the plant root is thought to be crucial for plant-beneficial effect particular Pseudomonas strains. Since often limiting step successful growth stimulation, this process needs improvement. It therefore important acquire more information as regards (i) conditions in rhizosphere, and (ii) bacterial traits that are involved colonization. This review discusses some recent studies focus on these two issues.
We describe the characterization of a novel Tn5lacZ colonization mutant efficiently colonizing Pseudomonas fluorescens strain WCS365, PCL1210, which is at least 300- to 1,000-fold impaired in potato root tip after co-inoculation stem cuttings with 1:1 mixture and parental cells. Similarly, also tomato, wheat, radish, indicating that gene involved plays role ability P. WCS365 colonize wide range plant species. A 3.1-kb DNA fragment was found be able complement observed mutation. The...
The use of bioluminescence as a sensitive marker for detection Pseudomonas spp. in the rhizosphere was investigated. Continuous expression luxCDABE genes, required bioluminescence, not detectable rhizosphere. However, when either naphthalene-inducible construct or constitutive luxAB (coding only luciferase) introduced into cells, light emission could be initiated just prior to measurement by addition naphthalene substrate luciferase, n -decyl aldehyde, respectively. These cells successfully...
Summary The initial step in the uptake of iron via ferric pseudobactin by plant‐growth‐promoting Pseudomonas putida strain WCS358 is binding to a specific outer‐membrane protein. nucleotide sequence pupA structural gene, which codes for receptor, was determined. It contains single open reading frame potentially encodes polypeptide 819 amino acids, including putative N ‐terminal signal 47 acids. Significant homology, concentrated four boxes, found with TonB‐dependent receptor proteins...
The invasive alien species Ambrosia artemisiifolia (common or short ragweed) is increasing its range in Europe. In the UK and Netherlands, airborne concentrations of pollen are usually low. However, more than 30 grains per cubic metre air (above level capable to trigger allergic symptoms) were recorded Leicester (UK) Leiden (NL) on 4 5 September 2014. aims this study determine whether highly allergenic during episode could be result long distance transport, identify potential sources these...
The European Commission Cooperation in Science and Technology (COST) Action FA1203 "SMARTER" aims to make recommendations for the sustainable management of Ambrosia across Europe monitoring its efficiency cost-effectiveness. goal present study is provide a baseline spatial temporal variations airborne pollen that can be used evaluation this noxious plant. covers full range artemisiifolia L. distribution over (39°N-60°N; 2°W-45°E). Airborne data principal flowering period (August-September)...
Airborne pollen is a major cause of allergic rhinitis, affecting between 10 and 30% the population in Belgium, Netherlands, Luxembourg (Benelux). Allergenic produced by wind pollinating plants released relatively low to massive amounts. Current climate changes, combination with increasing urbanization, are likely affect presence airborne allergenic respect exposure intensity, timing as well duration. Detailed analysis long-term temporal trends at supranational scale may provide more...
Airborne pollen monitoring is of global socio-economic importance as it provides information on presence and prevalence allergenic in ambient air. Traditionally, this task has been performed by microscopic investigation, but novel techniques are being developed to automate process. Among these, DNA metabarcoding the highest potential increasing taxonomic resolution, uncertainty exists about whether results can be used quantify abundance. In study, shown that using trnL nrITS2 highly improved...
Abstract The advent of automatic pollen and fungal spore monitoring over the past few years has brought about a paradigm change. provision real-time information at high temporal resolution opens door to wide range improvements in terms products services made available widening end-users stakeholders. As technology methods mature, it is essential properly quantify impact on different end-user domains better understand real long-term benefits society. In this paper, we focus main where such...
Roots of potato plants (Solanum tuberosum cv Bintje) growing on low Fe nutrient solution developed the characteristic efficiency reactions, such as high ferric reductase activity, proton extrusion and increased root hair formation. from a tuber with sprout removed, when grown Fe-free solution, also expressed these reactions; transfer to iron-containing medium resulted in their complete disappearance within 10 days. 2% sucrose sterile Murashige-Skoog activity upon withholding formed cells. It...
The outer membrane proteins of a series fluorescent, root-colonizing, plant-growth-stimulating Pseudomonas spp. having been characterized (L. A. de Weger et al., J. Bacteriol. 165:585-594, 1986), the lipopolysaccharides (LPSs) these strains were examined. chemical composition LPSs three best-studied WCS358, WCS361, and WCS374 P. aeruginosa PAO1 as reference strain was determined appeared to differ from strain. 2,6-dideoxy-2-aminosugar quinovasamine most abundant compound in LPS WCS358....