A5051918331- Reproductive tract infections research
- Cervical Cancer and HPV Research
- Microbial Community Ecology and Physiology
- Urinary Tract Infections Management
- Hepatitis B Virus Studies
- Environmental DNA in Biodiversity Studies
- Genital Health and Disease
- Syphilis Diagnosis and Treatment
- Genomics and Phylogenetic Studies
- Hepatitis C virus research
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- HIV, Drug Use, Sexual Risk
- Plant Pathogens and Fungal Diseases
- Protist diversity and phylogeny
- Marine Biology and Ecology Research
- Urinary and Genital Oncology Studies
- Plant-Microbe Interactions and Immunity
- Marine Invertebrate Physiology and Ecology
- Liver Disease Diagnosis and Treatment
- Wastewater Treatment and Nitrogen Removal
- Fungal Plant Pathogen Control
- HIV/AIDS Research and Interventions
- Probiotics and Fermented Foods
- Adolescent Sexual and Reproductive Health
University of Applied Sciences Leiden
2021-2025
Leiden University
2021-2025
Naturalis Biodiversity Center
2014-2023
Amsterdam UMC Location University of Amsterdam
2013-2016
Public Health Service of Amsterdam
2008-2016
Amsterdam UMC Location Vrije Universiteit Amsterdam
2013
University Medical Center Utrecht
2013
University Medical Center
2013
University Hospital and Clinics
2013
National Institute for Public Health and the Environment
2013
ABSTRACT A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources artifactual sequence variation 16S rDNA clone libraries. At least 14% the recovered contained aberrations. Artifact were polymerase errors, mutational hot spot, cloning heteroduplexes chimeras. These data may partially explain high degree microheterogeneity typical clusters detected environmental
The protection, preservation and restoration of aquatic ecosystems their functions are global importance. For European states it became legally binding mainly through the EU-Water Framework Directive (WFD). In order to assess ecological status a given water body, biodiversity data obtained compared reference body. quantified mismatch determines extent potential management actions. current approach assessment is based on morpho-taxonomy. This has many drawbacks such as being time consuming,...
Introduction This prospective study aimed to the composition and structure of vaginal microbiota prior Chlamydia trachomatis infection. Methods A nested case–control was performed in 122 women, half which acquired C. within a year after their first visit. At visit, microbial communities were analysed using 16S rRNA sequencing context sociodemographic sexual risk behaviour information logistic regression. Results Five community state types (CSTs) identified. Four CSTs dominated by...
ABSTRACT The shell of the bivalve Montacuta ferruginosa , a symbiont living in burrow an echinoid, is covered with rust-colored biofilm. This biofilm includes different morphotypes bacteria that are encrusted mineral rich ferric ion and phosphate. aim this research was to determine genetic diversity phylogenetic affiliation bacteria. Also, possible roles microorganisms processes deposition within biofilm, as well their impact on biology bivalve, were assessed by phenotypic inference....
To date, women are most often diagnosed with bacterial vaginosis (BV) using microscopy based Nugent scoring or Amsel criteria. However, the accuracy is less than optimal. The aim of present study was to confirm identity known BV-associated composition profiles and evaluate indicators for BV three molecular methods.Evaluation carried out by 16S rRNA amplicon sequencing V5-V7 region, a tailor-made oligonucleotide-based microarray, PCR-based profiling technique termed IS-profiling, which on...
Objective: Anal and penile high-risk human papillomavirus (HPV) infection is associated with anogenital cancer, which especially common in HIV-infected MSM. We assessed HPV prevalence determinants Design: Analysis of baseline data from a prospective cohort study. Methods: MSM aged 18 years or older were recruited Amsterdam, the Netherlands. Participants completed risk-factor questionnaires. DNA was analyzed anal shaft self-swabs genotyped using sensitive PCR reverse line blot assay...
Background The heterogeneous nature of environmental DNA (eDNA) and its effects on species detection community composition estimates has been highlighted in several studies the past decades. Mostly context spatial distribution over large areas, fewer occasions looking at within a single body water. Temporal variation eDNA, similarly, mostly studied as seasonality, observing changes periods time, often only for small groups organisms such fish amphibians. Methods We analyzed compared...
Microbial cure of chlamydia proctitis (lymphogranuloma venereum [LGV] and non-LGV) with doxycycline treatment was evaluated by DNA RNA persistence in anal swab specimens. In LGV proctitis, persisted for up to 16 days. non-LGV undetectable after 7 These findings support the Centers Disease Control Prevention's recommendation a 21-day regimen 7-day proctitis. Delayed microbial should be considered improved regimens.
Recreational drug use is associated with high-risk sexual behavior and sexually transmitted infections (STIs). We assessed the prevalence of during sex associations between such STI (chlamydia, gonorrhea, or syphilis).During 3 periods in 2008 2009, attendees an clinic Amsterdam were interviewed about tested for STI. Associations sex-related separately heterosexual men, men who have (MSM), women. examined whether was after adjusting behavior.Nine hundred sixty-one 673 MSM, 1188 women...
Oral infection with human papillomavirus (HPV) is associated a subset of head and neck cancers. We compared prevalence of, risk factors for, oral HPV among HIV-negative HIV-infected MSM.Analysis baseline data from prospective cohort study.MSM aged 18 years or older were recruited three study sites in Amsterdam, the Netherlands. Participants completed self-administered risk-factor questionnaire. Oral-rinse gargle specimens analyzed for DNA genotyped using highly sensitive PCR reverse line...
<h3>Objectives</h3> Pharyngeal <i>Chlamydia trachomatis</i> (chlamydia) might contribute to ongoing chlamydia transmission, yet data on spontaneous clearance duration are rare. We examined the prevalence, clearance, chlamydial DNA concentration and genotypes of pharyngeal among clinic patients with sexually transmitted infection (STI). <h3>Methods</h3> Female at high risk for an STI who reported active oral sex male have men (MSM) were screened RNA using a nucleic acid amplification test. A...
Objectives: A large portion of anogenital cancers is caused by high-risk human papillomavirus (hrHPV) infections, which are especially common in HIV-infected men. We aimed to compare the incidence and clearance anal penile hrHPV infection between HIV-negative MSM. Design: Analyses longitudinal data from a prospective cohort study. Methods: MSM aged 18 years or older were recruited Amsterdam, Netherlands, followed-up semi-annually for 24 months. At each visit, participants completed...
The effects of single or multiple concordant HPV infections at various anatomical sites on type-specific seropositivity are currently unknown. In this cross-sectional study we assessed whether high-risk (i.e., anal canal, penile shaft, and oral cavity), as well sites, were associated with in HIV-positive HIV-negative MSM. MSM aged ≥18 years recruited Amsterdam, the Netherlands (2010–2011). Baseline anal, penile, samples analyzed for DNA genotyped using a highly sensitive PCR reverse line...
DNA-based identification through the use of metabarcoding has been proposed as next step in monitoring biological communities, such those assessed under Water Framework Directive (WFD). Advances have made field metabarcoding, but challenges remain when using complex samples. Uneven biomass distributions, preferential amplification and reference database deficiencies can all lead to discrepancies between morphological taxa lists. The effects different taxonomic groups on these issues...
Airborne pollen monitoring is of global socio-economic importance as it provides information on presence and prevalence allergenic in ambient air. Traditionally, this task has been performed by microscopic investigation, but novel techniques are being developed to automate process. Among these, DNA metabarcoding the highest potential increasing taxonomic resolution, uncertainty exists about whether results can be used quantify abundance. In study, shown that using trnL nrITS2 highly improved...
In the collective genomes (the metagenome) of microorganisms inhabiting Earth's diverse environments is written history life on this planet. New molecular tools developed and used for past 15 years by microbial ecologists are facilitating extraction, cloning, screening, sequencing these genomes. This approach allows to access study full range diversity, regardless our ability culture organisms, provides an unprecedented breadth natural products that encode. However, there no way mere...