A5069003037- Reproductive tract infections research
- Urinary Tract Infections Management
- Syphilis Diagnosis and Treatment
- Cervical Cancer and HPV Research
- Microbial infections and disease research
- Pelvic floor disorders treatments
- Blood groups and transfusion
- HIV, TB, and STIs Epidemiology
- Urinary and Genital Oncology Studies
- Genital Health and Disease
- Adolescent Sexual and Reproductive Health
- Infectious Disease Case Reports and Treatments
- HIV, Drug Use, Sexual Risk
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Orthopedic Infections and Treatments
- Pneumonia and Respiratory Infections
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Infections and Vaccines
- Urological Disorders and Treatments
- Infective Endocarditis Diagnosis and Management
- Bartonella species infections research
- Reproductive Health and Contraception
- Diagnosis and treatment of tuberculosis
- Diphtheria, Corynebacterium, and Tetanus
Institut Polytechnique de Bordeaux
2012-2024
Centre Hospitalier Universitaire de Bordeaux
2012-2023
Université de Bordeaux
2012-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2011-2021
Hôpital Pellegrin
2005-2019
Institut National de la Recherche Agronomique
2011-2019
Institut National de la Recherche Agronomique du Niger
2012-2019
Hôpital Saint-Louis
2016-2017
Saint Louis University Hospital
2016
Assistance Publique – Hôpitaux de Paris
2016
The polymerase chain reaction (PCR) technique was used to detect Mycoplasma pneumoniae. A specific DNA sequence for M. pneumoniae selected from a genomic library, and two oligonucleotides were chosen in this give an amplified fragment of 144 base pairs. We show that different strains can be detected by PCR, with other species giving negative results. Analysis biological samples (throat swabs) obtained hamsters experimentally infected showed PCR more sensitive reliable than conventional...
BACKGROUND : Transfusion‐associated bacterial contamination (TABC), probably the most frequent transfusion‐transmitted infection, may induce serious adverse events. Systematic information and documentation on determinants are lacking. STUDY DESIGN AND METHODS The B acthem Study is a French matched case‐control study assessing TABC determinants. Included were cases of reported in France 2‐year period, as determined from uniform definitions. Information recipient‐, blood component‐,...
Streptococci are not an infrequent cause of periprosthetic joint infection (PJI). Management by debridement, antibiotics, and implant retention (DAIR) is thought to produce a good prognosis, but little known about the real likelihood success. A retrospective, observational, multicenter, international study was performed during 2003–2012. Eligible patients had streptococcal PJI that managed with DAIR. The primary endpoint failure, defined as death related infection, relapse/persistence or...
Abstract New or important issues in this updated version of the 2013 European guideline on management lymphogranuloma venereum (LGV): Epidemiology Lymphogranuloma continues to be endemic among men who have sex with (MSM) since 2003. infections heterosexuals are extremely rare Europe, and there is no evidence transmission LGV heterosexual population. Aetiology Chlamydia trachomatis serovars/genovars L2b L2 causative strains majority cases Europe. Clinical features Among MSM, about 25%...
ABSTRACT The mechanisms of intrinsic resistance Mycoplasma hominis to 14- and 15-membered macrolides were investigated in comparison with those M. pneumoniae , which is naturally susceptible macrolides. Radiolabeled erythromycin was not accumulated by PG21, but addition an ABC transporter inhibitor increased the level uptake more than two times, suggesting existence active efflux process. affinity [ 14 C]erythromycin ribosomes isolated from dramatically reduced relative that pneumoniae....
Respiratory tract specimens, 75 bronchoalveolar lavage (BAL) samples and 19 throat swabs from hospitalized adult patients (of whom 55 were seropositive for human immunodeficiency virus) with pulmonary infiltrates, examined Mycoplasma pneumoniae genitalium by culture polymerase chain reaction (PCR). Urogenital specimens (103 48 male nongonococcal urethritis female genital infections) M. the same techniques. Cultures of all negative. The oligonucleotides chosen published nucleotide sequences...
Few studies have estimated Chlamydia trachomatis (CT) prevalence in the general population, most being based on people already attending healthcare settings.To estimate of CT France, assess feasibility home sampling without any face-to-face intervention and identify risk factors associated with infection using data from Contexte de la Sexualité (CSF) survey sexual behaviour; a national population-based survey, carried out by telephone 2006.A random subsample sexually experienced aged 18-44...
Eight cases of psittacosis due to Chlamydia psittaci were identified in May 2013 among 15 individuals involved chicken gutting activities on a mixed poultry farm France. All women between 42 and 67 years-old. Cases diagnosed by serology PCR respiratory samples. Appropriate treatment was immediately administered the eight hospitalised after exposure birds had been discovered. In flocks, mainly C. gallinacea detected, new member family Chlamydiaceae, whereas ducks found harbour predominantly...
ABSTRACT The L2 reference strain of Chlamydia trachomatis was exposed to subinhibitory concentrations ofloxacin (0.5 μg/ml) and sparfloxacin (0.015 select fluoroquinolone-resistant mutants. In this study, two resistant strains were isolated after four rounds selection. C. mutants presented with high-level resistance various fluoroquinolones, particularly sparfloxacin, for which a 1,000-fold increase in the MICs mutant compared MIC susceptible found. unrelated antibiotics (doxycycline...
Restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 153 strains Mycoplasma pneumoniae isolated in France between 1977 and 1994, Denmark 1962 an additional group 28 from Belgium Germany 1990 1993. Random polymorphic DNA (RAPD) tested on French, Belgian German strains. Both methods separated into two groups corresponding reference M129 (group I) FH II), gave concordant results. When 75 selected different geographical origin were analysed by...
A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis amplified genes the major outer membrane protein (MOMP). Reference 15 serovars (A through K and L1 L3) clinical isolates were tested. The nucleotide sequences MOMP each arbitrarily constructed four variable domains known serovar constant L1. Computer these indicated that two digestions performed in parallel, one with AluI other IIpaII, followed HinfI EcoRI, would allow...
Chlamydia psittaci and C. gallinacea are obligate intracellular bacteria infecting poultry. We conducted a survey in two poultry slaughterhouses that were processing either exclusively ducks (A) or various species except (B). Cloacal swabs collected from all incoming flocks the course of week, blood samples pharyngeal taken workers. Swabs examined using PCR sera analyzed with immunoassays. testing revealed presence 9/38 duck complete absence these (slaughterhouse A), whereas 16/33...
Chlamydia psittaci is a zoonotic pathogen associated primarily with avian chlamydiosis also referred as psittacosis. Human psittacosis can lead to severe cases of respiratory disease. The mule duck one the main bird hybrids human in France. In order better understand epidemiology chlamydiosis, monitoring studies were performed both breeder flocks and flocks. Surveys conducted professional bredding organization revealed little shedding flocks, whereas heavy but asymptomatic C. was observed...
We describe a change in the molecular epidemiology of Chlamydia trachomatis strains involved an outbreak rectal lymphogranuloma venereum France during January 2010-April 2015. Until 2012, C. L2b strain predominated; however, starting 2013, most cases L2 strain. also identified 4 genetic ompA variants.
ABSTRACT Five clinical isolates of Mycoplasma hominis from three different patients were examined for resistance to fluoroquinolones; some these probably identical. All five harbored amino acid substitutions in the quinolone resistance-determining regions both DNA gyrase (GyrA) and topoisomerase IV (ParC or ParE). Furthermore, novobiocin MIC showed a significant increase. This is first characterization fluoroquinolone-resistant mycoplasma humans.
This report describes the case of a patient with 14-month course severe oligoarthritis associated acne. Pure cultures Propionibacterium acnes were isolated from synovial tissue and fluid specimens collected same joint after 4-month interval. After 2 months treatment roxithromycin 300 mg/day, rifampicin 1,200 nonsteroidal antiinflammatory drug (NSAID), followed by 4 azithromycin 1 gm/week NSAID, synovitis persisted. Cultures skin lesions at this time negative. Although P has previously been...
The rates of colonization by Ureaplasma urealyticum and Mycoplasma hominis were evaluated in 208 women at delivery their neonates. Mycoplasmas isolated from the cervicovaginal specimens 100 mothers (48.1%) gastric secretions 40 neonates (19.2%). prevalences U. M. 47.6% 11.0% 19.2% 1.0% neonates, respectively. Premature rupture membranes was significantly associated with (P = 0.031), 0.002) and/or 0.023). Forty-four selected strains mycoplasmas further characterized arbitrarily primed...
Diagnoses of bacterial sexually transmitted infections (STI) have been increasing in France since their resurgence the late 1990s. This article presents recent epidemiological trends until 2016 and patients' characteristics. STI surveillance relies on sentinel networks: a clinician-based network RésIST (clinical, biological behavioural data for early syphilis gonorrhoea), lymphogranuloma venereum (LGV) rectal LGV, laboratory networks Rénachla Rénago (demographic chlamydial gonorrhoea,...