A5053630816- Reproductive tract infections research
- Microbial infections and disease research
- Genital Health and Disease
- Blood groups and transfusion
- Syphilis Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Pneumonia and Respiratory Infections
- Urinary Tract Infections Management
- Cervical Cancer and HPV Research
- Pelvic floor disorders treatments
- Bacterial Infections and Vaccines
- Reproductive Health and Contraception
- Streptococcal Infections and Treatments
- Adolescent Sexual and Reproductive Health
- Urologic and reproductive health conditions
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Reproductive System and Pregnancy
- Urological Disorders and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Urinary and Genital Oncology Studies
- Antimicrobial Resistance in Staphylococcus
- Gut microbiota and health
Statens Serum Institut
2016-2025
Monash University
2018-2022
The University of Melbourne
2018-2022
University of Washington
2022
University of Alabama at Birmingham
2022
Tulane University
2022
ORCID
2021
Alfred Health
2018-2020
Royal Children's Hospital
2020
Royal Women's Hospital
2018-2020
Gonorrhoea is a major public health concern globally. Of particularly grave that resistance to the extended-spectrum cephalosporins has emerged during most recent years. This guideline provides recommendations regarding diagnosis and treatment of gonorrhoea in Europe. Compared outdated 2009 European guideline, this 2012 up-to-date guidance on, broader indications for testing gonorrhoea; introduction dual antimicrobial therapy (ceftriaxone 500 mg azithromycin 2 g) uncomplicated when...
Mycoplasma genitalium is a common cause of nongonococcal urethritis. Treatment trials have shown that doxycycline inefficient, whereas 5-day course azithromycin eradicates the bacterium from 95% infected men. The aim study was to establish reason for occasional treatment failures.Seven M. strains isolated men who experienced failure were tested in vitro susceptibility macrolides with use cell culture-based method. genetic basis drug resistance established by sequencing parts 23S ribosomal...
Four common pathological conditions are associated with vaginal discharge: bacterial vaginosis, aerobic vaginitis, candidosis, and the sexually transmitted infection, trichomoniasis. Chlamydial or gonococcal cervical infection may result in discharge. Vaginal discharge be caused by a range of other physiological including atrophic desquamative inflammatory cervicitis, mucoid ectopy. Psychosexual problems present recurrent episodes vulval burning. These need to considered if tests for...
Background and Objective Bacterial vaginosis (BV) is the most common vaginal disorder, characterized by depletion of normal lactobacillus-dominant microbiota overgrowth commensal anaerobic bacteria. This study aimed to investigate composition in women reproductive age (healthy with BV), view developing molecular criteria for BV diagnosis. Materials Methods Vaginal samples from 163 (79 control, 73 11 intermediate (Lactobacillary grade II flora) cases) were analyzed using 454 pyrosequencing...
What is the diagnostic performance of qPCR assays compared with Nugent scoring for abnormal vaginal microbiota and predicting success rate IVF treatment? The patients can be characterized tests which may promising tools diagnosing prediction clinical pregnancy in treatment. Bacterial vaginosis (BV) a common genital disorder prevalence approximately 19% infertile population. BV often sub-clinical change from being Lactobacillus spp. dominated to more heterogeneous environment anaerobic...
Rising macrolide and quinolone resistance in Mycoplasma genitalium necessitate new treatment approaches. We evaluated outcomes of sequential antimicrobial therapy for M. guided by a macrolide-resistance assay. In mid-2016, Melbourne Sexual Health Centre switched from azithromycin to doxycycline (100 mg twice daily 7 days) nongonococcal urethritis, cervicitis, proctitis. Cases were tested mutations (MRMs) polymerase chain reaction. Directly after doxycycline, MRM-negative infections received...
Gonorrhoea is a major public health concern globally. Increasing incidence and sporadic ceftriaxone-resistant cases, including treatment failures, are growing concerns. The 2020 European gonorrhoea guideline provides up-to-date evidence-based guidance regarding the diagnosis of gonorrhoea. updates recommendations emphasize significantly increasing incidence; broad indications for increased testing with validated quality-assured nucleic acid amplification tests culture; dual antimicrobial...
Mycoplasma genitalium infection contributes to 10-35% of non-chlamydial non-gonococcal urethritis in men. In women, M. is associated with cervicitis and pelvic inflammatory disease (PID) 10-25%. Transmission occurs through direct mucosal contact.Asymptomatic infections are frequent. men, urethritis, dysuria discharge predominate. symptoms include vaginal discharge, or PID - abdominal pain dyspareunia. Symptoms the main indication for diagnostic testing. Diagnosis achievable only nucleic acid...
ABSTRACT Mycoplasma genitalium is a cause of nongonococcal urethritis, particularly in patients not infected with Chlamydia trachomatis . A quantitative 5′ nuclease assay (TaqMan PCR) was developed and validated. The detected fragment the MgPa adhesin gene by use TaqMan MGB (minor groove binder) probe included an internal processing control to detect PCR inhibition. Urethral swab specimens first-void urine samples from M. -positive men were examined, DNA load correlated symptoms signs....
We have used the polymerase chain reaction to detect Mycoplasma genitalium in artificially seeded human throat swab samples as well clinical material. On basis of published nucleotide sequence M. 140-kDa adhesin gene, primers were chosen produce an amplified fragment 281 bp. Five different previously isolated strains, including type strain genitalium, could all be detected by reaction, and DNAs from other mycoplasmal bacterial species yielded negative results. The detection limits estimated...
Use of the heptavalent pneumococcal conjugate vaccine (PCV-7 [Prevnar]) has been associated with decreased a incidence invasive disease (IPD) among children in United States.Cases IPD < 18 years age insured by or receiving health care from Intermountain Health Care during 1996-2003 were identified. Isolates S. pneumoniae treated at Primary Children's Medical Center (PCMC; Salt Lake City, UT) 1997-2003 serogrouped. Temporal trends IPD, serogroup distribution pneumococci, and antibiotic...
Background. Our aim was to determine the efficacy of 1 g azithromycin and alternative antibiotic regimens in a prospective cohort Mycoplasma genitalium–infected participants, factors associated with failure. Methods. Consecutive eligible M. men women attending Melbourne Sexual Health Centre between July 2012 June 2013 were treated retested by polymerase chain reaction (PCR) on days 14 28. Cure defined as PCR negative day Cases failing moxifloxacin, those moxifloxacin pristinamycin. Pre-...
Abstract We report significant failure rates (28%, 95% confidence interval 15%–45%) after administering 1 g azithromycin to men with Mycoplasma genitalium–positive nongonococcal urethritis. In vitro evidence supported reduced susceptibility of M. genitalium macrolides. Moxifloxacin administration resulted in rapid symptom resolution and eradication infection all cases. These findings have implications for management
The role of Mycoplasma genitalium in pelvic inflammatory disease is unclear. We conducted a cohort study to determine the prevalence and predictors M. infection female students, explore its estimate annual incidence persistence rate.Two thousand three hundred seventy-eight multiethnic, sexually active students (mean age, 21 years) provided duplicate self-taken vaginal samples for chlamydia screening trial. From this population, 2246 (94%) were followed up after 12 months assessed clinical...
Abstract Escalating resistance to azithromycin and moxifloxacin is being reported for Mycoplasma genitalium in the Asia-Pacific region. Analyzing 140 infections, we found pretreatment fluoroquinolone-resistance mutations parC (13.6%) gyrA (5%). ParC S83 changes were associated with failure. Combined macrolide/fluoroquinolone-resistance 8.6% of specimens, which recommended therapies would be ineffective.
Background. Mycoplasma genitalium is a common cause of nongonococcal urethritis (NGU) and cervicitis. The aim the study was to analyze M. testing pattern distribution positive results according sex age in 5-year period where all diagnostic Denmark centralized at Statens Serum Institut. A secondary estimate occurrence macrolide resistance 3-year period.