A5102724914- Molecular Biology Techniques and Applications
- Reproductive Health and Technologies
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Click Chemistry and Applications
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- Liver physiology and pathology
- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- Radiation Dose and Imaging
- Gene expression and cancer classification
- Ecology and biodiversity studies
- Bioactive Compounds and Antitumor Agents
- Aquatic Invertebrate Ecology and Behavior
- Synthesis and Biological Evaluation
- Animal Disease Management and Epidemiology
- Fish Ecology and Management Studies
- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- Plant and fungal interactions
South Dakota State University
2004
National Institute for Occupational Safety and Health
2002
Oncology Hematology Care
2002
University of Arizona
1994
Memorial Sloan Kettering Cancer Center
1979-1984
Kettering University
1978
Abstract The metachromatic fluorochrome acridine orange was used to differentially stain DNA and RNA in Chinese hamster ovary (CHO) cells mitogen‐stimulated human lymphocytes during their progression through the cell cycle. Green red fluorescence of individual cells, representing cellular RNA, respectively, measured by flow cytometry. CHO were synchronized selective detachment at mitosis. Their rate G 1 subsequently S phase correlated with content stainable RNA. mean duration 5.2 hours for...
A flow cytometric technique for simultaneous measurements of RNA and DNA in individual cells has been applied to correlate the content cellular with rate progression through S phase. Human peripheral lymphocytes stimulated phytohemagglutinin were blocked at G1/S phase boundary by hydroxyurea or 5-fluorodeoxy-uridine treatment. Cells G1 as well showed high heterogeneity respect stainable content. After release from block, traversed rates proportional quantity per cell. highest completed...
Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of World Health Organization laboratory manual for examination and processing human semen. Many conditions risk factors have been associated with elevated SDF; therefore, it is important identify population infertile men who might benefit from this test. The purpose study investigate global practices related indications SDF testing, compare relevant professional society guideline recommendations, provide expert...
We examined the genetic composition, habitat use, and morphological variation of a Phoxinus eos-neogaeus unisexual hybrid complex its sexually reproducing progenitor species inhabiting beaver-modified drainages Voyageurs National Park, Minnesota. In addition to single diploid P. gynogenetic clone, triploid diploid-triploid mosaic biotypes were present at our study sites. Both eos neogaeus, all three ubiquitous throughout one intensively surveyed drainage, but abundances relative frequencies...
The RNA components of two C-type viruses, avian myeloblastosis virus and Friend leukaemia virus, have been isolated by treatment the viruses with 6 M-guanidine-HCl precipitation ethanol. proteins were recovered lyophilization guanidine-HCl-ethanol supernatant after thorough dialysis against 0.5 mM-dithiothreitol. This simple method yielded similar quality to phenol sodium dodecyl sulphate (SDS) extraction methods, same amount 60-70S RNA, although a fraction smaller (4S) species remained in...
To define the in vitro response of nonparenchymal liver cells to chemical carcinogens, an endothelial-like cell line (RLC-1) derived from adult rat hepatic tissue was established long-term culture. The RLC-1 exhibited morphologic, ultrastructural and cytoskeletal characteristics consistent with derivation mesenchymal vascular elements within liver. Using various criteria monitor changes growth properties, were determined be resistant transformation by diethylnitrosamine, partially...
Grajewski, Barbara PhD; Cox, Clinton Schrader, Steven M. Murray, William E. MS; Edwards, Richard BS; Turner, Terry W. Smith, James Evenson, Donald P. Simon, Stephen D. Conover, David L. PhD Author Information