A5054912605- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Parasite Biology and Host Interactions
- Identification and Quantification in Food
- Vibrio bacteria research studies
- Aquatic life and conservation
- Marine Ecology and Invasive Species
- Parasites and Host Interactions
- Fish Biology and Ecology Studies
- Myxozoan Parasites in Aquatic Species
- Marine Bivalve and Aquaculture Studies
- Meat and Animal Product Quality
- Reproductive biology and impacts on aquatic species
- Mycobacterium research and diagnosis
- Microbial Metabolism and Applications
- Seaweed-derived Bioactive Compounds
- Microbial infections and disease research
- Immune Response and Inflammation
- Echinoderm biology and ecology
- Parasitic infections in humans and animals
- RNA Interference and Gene Delivery
- Biochemical Analysis and Sensing Techniques
- Bioactive Compounds and Antitumor Agents
- Medicinal Plant Research
- Natural product bioactivities and synthesis
Fondazione Centro Marino Internazionale
2020-2022
Istituto Zooprofilattico Sperimentale della Sardegna
2009
Università di Camerino
1999
Kōchi University
1984-1993
Institut National de la Recherche Agronomique du Niger
1989
University of Turin
1984
The properties and taxonomic position of bacterial strains isolated from diseased specimens cultured yellowtail eels were examined. isolates gram-positive, short-chain-forming, catalase-negative, facultatively anaerobic cocci. Growth at 10 45°C in 6.5% NaCl (pH 9.6) with 40% bile 0.1% methylene blue-milk both positive. could be distinguished other species the genus Enterococcus by several biochemical characteristics Lancefield's group antigen. Guanine-plus-cytosine content DNA was 44 mol% as...
Following the Mass Mortality Events (MMEs) of pen shell P. nobilis in Campania region and Sicily, a survey moribund specimens was also conducted other Italian regions (Campania, Tuscany, Sardinia Apulia) Spain (Catalunya). Histopathological molecular examination 27 nobils revealed different types pathogens associated with tissue lesions, morbidity mortality. Presence Mycobacterium, Vibrio species, Haplosporidium pinnae Perkinsus sp. were detected, differently distributed into areas. The...
Anisakis spp. larvae are parasitic, and potentially zoonotic, nematodes transmitted by marine fish cephalopods, which the main intermediate hosts of third larval stage. The accidental consumption infected raw or poorly cooked may cause gastroenteric diseases allergies in humans. aim present study was to use polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) define occurrence, species variability, host preferences caught off coast Sardinia. Necropsy used on 285...
Red sea bream, Pagrus major, were injected intramuscularly once with Edwardsiella tarda crude lipopolysaccharide (LPS) and formalin killed cells (FKC) preparations. The immune response of red bream was studied 1, 2, 3 4 weeks after the immunization. protein, albumin glucose serum, agglutination antibody titer, passive hemagglutination complement-like factor activity, bacteriolytic activity serum skin mucus in vitro phagocytic index total blood immunized fish determined.The results analysis...
From Edwardsiella tarda EF-1 strain, three antigens were obtained: lipopolysaccharide (LPS), culture filtrate and formalin killed whole cells. Eel injected intramuscularly with the twice at an interval of one week. The agglutination passive hemagglutination titers in serum determined weeks after second immunization. In another experiment, eel challenged by intramuscular injection live bacteria immunization titer was surviving eels. Antigenic relationships between LPS examined OUCHTERLONY...
Two different batches of meagre Argyrosomus regius were stocked in the same floating cage located north-eastern Sardinia (western Mediterranean Sea). After 4 mo, fish started showing non-specific disease signs, such as lethargy, emaciation, gill anaemia and mortality. In total 65 specimens both stocks sampled for parasitological microbiological analysis, only 1 species parasite, microcotylid monogenean Sciaenacotyle panceri, was found on gills caged meagre. No protozoan or other metazoan...
Mycobacterium marinum is the causative agent of mycobacteriosis in wild and cultured fish atypical infection humans. For diagnosis M. marinum, cultural traditional polymerase chain reaction (PCR) methods are currently used. However, these protocols, although able to discriminate within spp., have proved be time-consuming or difficult carry out. this reason, aim study was obtain a rapid specific diagnostic tool quantify spp. from other mycobacteria. A primary PCR amplification with SYBR Green...
Currently, presence of Moraxella sp. in internal organs fish is not considered detrimental for farming. However, bacterial colonization can affect wellness and decrease growth rate, stress resistance, immune response. Recently, there have been reports by farmers concerning slow growth, poor feed conversion, low average weight increase farmed offshore floating sea cages, often associated with organ Therefore, these opportunistic bacteria deserves further investigations elucidating incidence...
To clarify the protective properties of Edwardsiella tarda lipopolysaccharide (LPS) reported previously, immunogenicity, antigenicity and chemical characteristics were examined using crude LPS, pure polysaccharide lipid A prepared from one strain E. tarda. The eels immunized by intramuscular injection with four preparations showed an increase in agglutination titer comparison to control group. highest titers recorded LPS preparation lowest preparation. However, did not correlate survival...
Abstract In order to study the effects of bath vaccination against vibriosis in European sea bass fingerlings, Dicentrarchus labrax L. 1758, reared Sardinia land-based concrete tanks and cages, a commercial preparation was used. The achievement immune response, presence disease growth performances were determined monthly one year study. results showed an increase antibody titers immunized fish when compared controls. Moreover, although spreads every farms, also during trials year, no...
Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics have captured ever-increasing market shares. However, the expansion of molluscan culture worldwide global trade favored spread pathogens around world. Combined with environmental changes intensive production systems this contributed to occurrence mass mortality episodes, thus posing a threat different species, including Pacific oyster...
To confirm the properties of lipid from Edwardsiella tarda as immunosuppressor in eel, suggested by previous studies, extracted whole cell (Lipid), crude lipopolysaccharide (LPS), formalin killed (FKC) and without (CWL) were injected intramuscularly eels. compare protective effect preparations, eels challenged with live E. tarda, strain EF-1.The immunized Lipid preparation did not show differences protection against challenge comparison control group, highest survival rate was recorded LPS...
Abstract Innate and acquired immune responses of Gilthead Seabream Sparus aurata was studied under normal culture short-term stressful conditions for 18 months in offshore sea cages Alghero Bay, Italy. Every 45 d, 50 fish were sampled divided into two groups: the first group (normal conditions) bled after harvesting; second put a tank (crowding confinement) 2 h. humoral immunity, such as complement-like, hemagglutination, lysozyme activities, determined sera both groups. Pathogen challenge...
Chemical analysis and toxic ty assay of Pasteurella piscicida lipopolysaccharide (LPS) preparations used for immunization yellowtail (Seriola quinqueradiata) were performed. LPS fractions obtained by the phenol-water (P-LPS) or phenol-chloroform-petroleum ether (PCP-LPS) methods followed purification with Cetavlon ultracentrifugation, respectively. P-LPS was composed 0.87% protein, 24.0% sugar 36.0% fatty acid. Whereas PCP-LPS 0.34% 18.0% 34.2% The monosaccharide respectively : 30.6% 16.5%...