A5026086160- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Diversity and Evolution
- Plant-Microbe Interactions and Immunity
- Cellular transport and secretion
- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Antimicrobial Peptides and Activities
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Enzyme-mediated dye degradation
- Ubiquitin and proteasome pathways
- Biochemical and Structural Characterization
- RNA modifications and cancer
- Plant and Fungal Species Descriptions
- Insect and Pesticide Research
- Gut microbiota and health
- Sirtuins and Resveratrol in Medicine
- Redox biology and oxidative stress
- Genomics and Phylogenetic Studies
- Pharmacological Effects of Natural Compounds
- Drug Transport and Resistance Mechanisms
Sichuan Agricultural University
2017-2025
Army Medical University
2025
California State University, Northridge
2025
Daping Hospital
2025
Guangzhou Medical University
2023-2024
The Central Hospital of Xiao gan
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
State Key Laboratory of Respiratory Disease
2023
China Three Gorges University
2021
Chengdu University of Traditional Chinese Medicine
2020
Wortmannin, a specific inhibitor of phosphatidyl-inositol 3-kinase, is useful tool for studying protein trafficking and identifying organelles in the plant secretory endocytic pathways. It has recently been demonstrated that wortmannin at 16.5 microM or 33 caused prevacuolar compartments (PVCs), identified as multivesicular bodies (MVBs) by their enrichment vacuolar sorting receptor (VSRs) proteins BP-80 reporter, to form small vacuoles rapidly. However, source(s) membrane needed rapid...
Abstract Background We developed a computer-assisted diagnosis model to evaluate the feasibility of automated classification intrapapillary capillary loops (IPCLs) improve detection esophageal squamous cell carcinoma (ESCC). Methods recruited patients who underwent magnifying endoscopy with narrow-band imaging for evaluation suspicious condition. Case images were evaluated establish gold standard IPCL according endoscopic and histological findings. A double-labeling fully convolutional...
Abstract Genetic engineering using negative regulators of plant immunity has the potential to provide a huge impetus in agricultural biotechnology achieve higher degree disease resistance without reducing yield. Type 2C protein phosphatases (PP2Cs) represent largest group plants, with high for regulatory functions by blocking transmission defence signals through dephosphorylation. Here, we established PP2C functional protoplast screen pFRK1 :: luciferase as reporter and found that 14 56...
The edible straw mushroom, Volvariella volvacea, produces a multicomponent enzyme system consisting of endo-1,4-beta-glucanase, cellobiohydrolase, and beta-glucosidase for the conversion cellulose to glucose. highest levels endoglucanase cellobiohydrolase were recorded in cultures containing microcrystalline (Avicel) or filter paper, while lower but detectable activity also produced on carboxymethyl cellulose, cotton wool, xylitol, salicin. Biochemical analyses different culture fractions...
Summary Brefeldin A (BFA) is a useful tool for studying protein trafficking and identifying organelles in the plant secretory endocytic pathways. At low concentrations (5–10 μg ml −1 ), BFA caused both Golgi apparatus trans ‐Golgi network (TGN), an early endosome (EE) equivalent cells, to form visible aggregates transgenic tobacco BY‐2 cells. Here we show that these BFA‐induced from TGN are morphologically functionally distinct Confocal immunofluorescent immunogold electron microscope (EM)...
Membrane proteins on the tonoplast are indispensible for vacuolar functions in plants. However, how these transported to vacuole and they become separated from plasma membrane remain largely unknown. In this study, we used Arabidopsis thaliana ion transporter1 (VIT1) as a reporter study mechanisms of targeting. We showed that VIT1 reached through pathway involving endoplasmic reticulum (ER), Golgi, trans-Golgi network (TGN), prevacuolar compartment, tonoplast. contains putative N-terminal...
In contrast to a single copy of Exo70 in yeast and mammals, the Arabidopsis genome contains 23 paralogues (AtExo70). Using AtExo70E2 its GFP fusion as probes, we recently identified novel double-membrane organelle termed exocyst-positive (EXPO) that mediates an unconventional protein secretion plant cells. Here further demonstrate is essential for exocyst subunit recruitment EXPO formation both plants animals. By performing transient expression protoplasts, established number subunits...
bHLH (basic helix-loop-helix) transcription factors play important roles in the abiotic stress response plants, but their characteristics and functions tartary buckwheat (Fagopyrum tataricum), a flavonoid-rich cereal crop with strong tolerance, have not been fully investigated. Here, novel gene, designated FtbHLH3, was isolated characterized. Expression analysis revealed that FtbHLH3 mainly induced by polyethylene glycol 6000 (PEG6000) abscisic acid (ABA) treatments. Subcellular localization...
We have established an efficient transient expression system with several vacuolar reporters to study the roles of endosomal sorting complex required for transport (ESCRT)-III subunits in regulating formation intraluminal vesicles prevacuolar compartments (PVCs)/multivesicular bodies (MVBs) plant cells. By measuring distributions on/within membrane PVC/MVB or tonoplast, we identified dominant negative mutants ESCRT-III that affect protein degradation from both secretory and endocytic...
Conyza blinii H. Lév is a medicinal plant that has variety of pharmacological activities, but its study at standstill due to the shortage resources.This utilized surface sterilization method isolate endophytic fungi, and they were preliminarily identified by morphology. Flavonoid-producing strains screened NaNO2-Al(NO)3 colorimetry further ITS sequence. Additionally, we used five antioxidant assays (DPPH, Hydroxyl radical, ABTS, FRAP T-AOC assays) systematically evaluate capacity total...
Summary How polytopic plasma membrane (PM) proteins reach their destination in plant cells remains elusive. Using transgenic tobacco BY‐2 cells, we previously showed that the rice secretory carrier protein 1 (SCAMP1), an integral with four transmembrane domains (TMDs), is localized to PM and trans ‐Golgi network (TGN). Here, study transport pathway sorting signals of SCAMP1 by following its transient expression protoplasts show reaches via endoplasmic reticulum (ER)–Golgi–TGN–PM pathway....
In tip-confined growing pollen tubes, delivery of newly synthesized cell wall materials to the rapidly expanding apical surface requires spatial organization and temporal regulation F-actin filament exocytosis. this study, we demonstrate that is essential for rigidity construction tube by regulating exocytosis Nicotiana tabacum pectin methylesterase (NtPPME1). Wortmannin disrupts in tip inhibits polar targeting NtPPME1, which subsequently alters pectic composition wall, finally causing...
The CLE (CLAVATA3/Embryo Surrounding Region-related) family, a group of peptides with hormone-like features, plays pivotal role in plant growth, development, and adaptation to stress. Through homology-based blast analysis 32
Intralesional injection of low-dose 5-fluorouracil (5-FU) has recently been used as an experimental modality for treating keloid scarring and shown promising efficacy in improving scar appearance preventing recurrence the keloid.We sought to explore cellular- molecular-based evidence observed clinical benefits.Primary cell lines fibroblasts were treated with 5-FU at a range lower doses (∼10 mg mL(-1) ) monolayer culture subjected examination viability, proliferative potential, apoptosis,...
Arabidopsis thaliana ARA7 (AtRabF2b), a member of the plant Rab5 small GTPases functioning in vacuolar transport pathway, localizes to pre-vacuolar compartments (PVCs), known as multivesicular bodies (MVBs) cells. Overexpression constitutively active GTP-bound mutant ARA7, ARA7(Q69L), induces formation large ring-like structures (1–2 µm diameter). To better understand biology these ARA7(Q69L)-induced structures, transgenic cell lines expressing ARA7(Q69L) tagged with green fluorescent...
We previously demonstrated that rice (Oryza sativa) SECRETORY CARRIER MEMBRANE PROTEIN1 (OsSCAMP1)-yellow fluorescent protein in transgenic tobacco (Nicotiana tabacum) Bright Yellow-2 cells locates to the plasma membrane and motile punctate structures, which represent trans-Golgi network/early endosome are tubular-vesicular nature. Here, we now show SCAMPs diverted cell plate during cytokinesis dividing cells. As progress from metaphase cytokinesis, OsSCAMP1-labeled structures begin collect...
In plant cells, how integral plasma membrane (PM) proteins are degraded in a cargo ubiquitination-independent manner remains elusive. Here, we studied the degradative pathway of two PM proteins: AtLRR84A, type I protein belonging to leucine-rich repeat receptor-like kinase family, and OsSCAMP1 (rice secretory carrier 1), tetraspan transmembrane located on trans-Golgi network (TGN) or early endosome (EE). Using wortmannin ARA7(Q69L) mutant that could enlarge multivesicular body (MVB)...
The objective of this study was to evaluate the diversity endophytic fungi different parts Ligusticum chuanxiong Hort (CX) and further characterize their biological activities identify chemical compounds produced by these fungi. A total 21 were isolated identified from CX. Penicillium oxalicum, Simplicillium sp., Colletotrichum sp. as promising strains color reaction. Comparing organic extracts three strains, it observed that ethyl acetate extract oxalicum n-butanol showed significant...