A5079535786- Cancer Research and Treatments
- Drug Transport and Resistance Mechanisms
- Cancer therapeutics and mechanisms
- Vibrio bacteria research studies
- Steroid Chemistry and Biochemistry
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Aquaculture disease management and microbiota
- Antimicrobial Peptides and Activities
- Cholesterol and Lipid Metabolism
- Legionella and Acanthamoeba research
- Oral microbiology and periodontitis research
- Bacterial Infections and Vaccines
- Escherichia coli research studies
- Probiotics and Fermented Foods
- Invertebrate Immune Response Mechanisms
- Essential Oils and Antimicrobial Activity
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- Barrier Structure and Function Studies
- Yersinia bacterium, plague, ectoparasites research
- Child Nutrition and Water Access
- Gut microbiota and health
- Microbial Metabolism and Applications
Université de Montréal
2019-2025
Umeå University
2013-2021
Ifremer
2010-2014
Université de Montpellier
2010-2012
Centre National de la Recherche Scientifique
2010-2012
Institut de Recherche pour le Développement
2011-2012
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen cholera , require for colonization and virulence. We have shown previously that is essential virulence in oyster splendidus LGP32. Here, we showed V. LGP32 invades immune cells, hemocytes, through subversion host-cell actin cytoskeleton. In this process, serves an adhesin/invasin required...
Healthy oysters are inhabited by abundant microbial communities that vary with environmental conditions and coexist immunocompetent cells in the circulatory system. In Crassostrea gigas oysters, antimicrobial response, which is believed to control pathogens commensals, relies on potent oxygen-dependent reactions peptides/proteins (AMPs) produced at low concentrations epithelial and/or circulating hemocytes. non-diseased hemocytes express basal levels of defensins (Cg-Defs) proline-rich...
Outer membrane vesicles (OMVs) that are released from Gram-negative pathogenic bacteria can serve as vehicles for the translocation of effectors involved in infectious processes. In this study we have investigated role OMVs Vibrio cholerae O1 El Tor A1552 strain resistance to antimicrobial peptides (AMPs). To assess potential role, grew V. with sub-lethal concentrations Polymyxin B (PmB) or AMP LL-37 and analyzed produced their effects on resistance. Our results show growing presence AMPs...
Summary Vibrio splendidus , strain LGP32, is an oyster pathogen associated with the summer mortalities affecting production of Crassostrea gigas oysters worldwide. LGP32 was shown to resist up 10 µM Cg ‐Def defensin and ‐BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. immunity. The resistance both standard AMPs (polymyxin B, protegrin, human BPI) dependent on ompU gene. Indeed, upon inactivation, minimal concentrations decreased by...
Summary Vibrio tasmaniensis LGP 32, a facultative intracellular pathogen of oyster haemocytes, was shown here to release outer membrane vesicles ( OMV s) both in the extracellular milieu and inside haemocytes. Intracellular s occurred phagosomes intact haemocytes having phagocytosed few vibrios as well damaged containing large vacuoles heavily loaded with 32. The proteome 32 be rich hydrolases (25%) including potential virulence factors such proteases, lipases, phospholipases, haemolysins...
Biofilm formation is a common strategy used by bacteria in order to survive and persist the environment. In Vibrio cholerae (V. cholerae), Gram-negative pathogen responsible for cholera disease, biofilm-like aggregates are important pathogenesis disease transmission. initiated attachment of surface, followed maturation stages involving biofilm matrix. V. cholerae, flagella essential initial step formation, allowing swim detect surface. this study, we explored effect polymyxin B (PmB),...
Background Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that may therefore be able transport bacterial proteases target host cells. present here an analysis of Vibrio cholerae OMV-associated protease PrtV. Methodology/Principal Findings In this study, we demonstrated PrtV was secreted wild type V. strain C6706 via II...
Climate change and ocean warming have a huge impact on microbial communities, leading to an increased prevalence of Vibrio parahaemolyticus infections in northern America. V. is inhabitant aquatic environments associated with fishes shellfishes, particularly oysters. It causes gastrointestinal infection through consumption contaminated seafood, as well wound or septicemia. Temperature known affect virulence persistence factors parahaemolyticus. In this study, twenty clinical strains isolated...
Vibrio cholerae includes strains responsible for the cholera disease and is a natural inhabitant of aquatic environments. V. possesses unique polar flagellum essential motility, adhesion, biofilm formation. In previous study, we showed that motility formation are altered in presence subinhibitory concentrations polymyxin B O1 O139. this performed an experimental evolution to identify genes restoring concentration B. Mutations five have been identified three variants derived from two...
<title>Abstract</title> Antimicrobial peptides are small cationic molecules produced by eukaryotic cells to combat infection, as well bacteria for niche competition. Polymyxin B (PmB), a cyclic antimicrobial peptide, is used prophylactically in livestock and last-resort treatment multidrug-resistant bacterial infections humans. In this study, transcriptomic analysis <italic>Vibrio cholerae</italic> showed that expression of the uncharacterized gene <italic>ompV</italic> stimulated response...
<p>Figure S1 shows E. coli metabolome and ornithine decarboxylase activity</p>
<p>Figure S3 shows the effect of putrescine on gut microbiota</p>
<p>Figure S4 shows gut microbiota changes after DSS-induced flares</p>
<p>Figure S2 shows the effects of putrescine supplementation on cell proliferation</p>
<p>Table S2 shows primers used</p>
<p>Table S1 shows cohort characteristics</p>
<p>Figure S5 shows the effect of putrescine on gut microbiota functions</p>
<p>Figure S6 shows growth of pks+ E. coli strains isolated from human samples</p>
<p>Figure S7. Putrescine lowers inflammation, inhibits pks+ E. coli growth, and reduces tumor development.</p>
Antimicrobials are commonly used in prevention of infections including aquaculture, agriculture and medicine. Subinhibitory concentrations antimicrobial peptides can modulate resistance, virulence persistence effectors Gram-negative pathogens. In this study, we investigated the effect subinhibitory polymyxin B (PmB) on secretome Vibrio cholerae, a natural inhabitant aquatic environments pathogen responsible for cholera disease. Our proteomic approach revealed that abundance many...