A5009284778- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
California Institute of Technology
2023-2024
Polycomb repressive complex 2 (PRC2) is reported to bind many RNAs and has become a central player in reports of how long non-coding (lncRNAs) regulate gene expression. Yet, there growing discrepancy between the biochemical evidence supporting specific lncRNA-PRC2 interactions functional demonstrating that PRC2 often dispensable for lncRNA function. Here, we revisit RNA binding by show may not occur vivo. Using denaturing purification vivo crosslinked RNA-protein complexes human mouse cell...
The nucleus is highly organized such that factors involved in transcription and processing of distinct classes RNA are within specific nuclear bodies. One body the speckle, which defined by high concentrations protein non-coding regulators pre-mRNA splicing. What functional role, if any, speckles might play process mRNA splicing remains unknown. Here we show genes localized near display higher spliceosome concentrations, increased binding to their pre-mRNAs, co-transcriptional levels...