A5003817688- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Animal Virus Infections Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Lipid metabolism and biosynthesis
- Hepatitis B Virus Studies
- Genomics and Phylogenetic Studies
California Institute of Technology
2020-2024
University of Nebraska–Lincoln
2018
Polycomb repressive complex 2 (PRC2) is reported to bind many RNAs and has become a central player in reports of how long non-coding (lncRNAs) regulate gene expression. Yet, there growing discrepancy between the biochemical evidence supporting specific lncRNA-PRC2 interactions functional demonstrating that PRC2 often dispensable for lncRNA function. Here, we revisit RNA binding by show may not occur vivo. Using denaturing purification vivo crosslinked RNA-protein complexes human mouse cell...
Current mitochondrial purification techniques are tedious and protracted due to their emphasis on recovering physiologically active mitochondria. However, for studies that exclusively interested in isolating DNA (mtDNA) applications such as PCR sequencing, respiring mitochondria − the complex procedures stem from need retain function unnecessary. Still, global extraction methods have proven insufficient isolation because nuclear segments (NUMTs) pose unique challenges accurate mtDNA...
Although thousands of lncRNAs are encoded in mammalian genomes, their mechanisms action largely uncharacterized because they often expressed at significantly lower levels than proposed targets. One such lncRNA is Xist, which mediates chromosome-wide gene silencing on one the two X chromosomes to achieve expression balance between males and females. How a limited number Xist molecules can mediate robust larger target genes (∼1 RNA: 10 targets) while maintaining specificity within each cell...